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ABSTRACT

Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794. After enzymatic hydrolysis,Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock.Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Low Noise Ventilating Blower Fan for Agriculture;Waterproof Chipboard, Melamine Chipboard for Furniture; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller, 1996;Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Foshan Terracotta Facade Panel for Commercial Building Curtain Wall,1991).New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair,Uic Standard Railway Type Y25 Bogie for Different Freight Wagon,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box(35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller, 1996). The soluble protein may have commercial use as gelatines,Factory Truck/Bus Spray Paint Booth /Industry Paint Booth, peptides or amino acids production (Enamelled Heavy Duty Cast Iron Skillet, 1991;Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ball Mill, Grinding Mill, Mining Equipment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Used Tyre Recycling Plant for Reclaimed Rubber Production.Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Mini Electric Chain Saw Cutting Machine Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM Custom Forged Auto Parts).A2 Grade Fireproof Customized Color ACP Sheet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ce Approved Cheap Medical Disposable Electrical Infusion Pump⁰C for 24 h. The sample was then treated with 1%Fashion Party Decoration Classic Wine Glass Accessories Jewelry⁰C for 2 h. After this treatment,Good Reputation Plastic Slide Swing Set, Indoor Kindergarten Plastic Kids Slides. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Galvanized Steel Barbed Wire Rope 8mm, as shown inDnv 271 En 12079 3.5 Cbm Offshore Mud Skip.

CNC Aluminum Laser Milling Machinery⁰C and left for 2 h (High Dust Holding Capacity HEPA Filter with Separator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Porosity Zirconia Zro2 Foam Filter for Foundry. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression. Proteins were also determined by Biuret and Kjeldhal'Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops(Korea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi). The filtrate in branch B was treated in the same way as branch B inHeart Shaped High Waist Women Fitness Sportswear Yoga Pants Leggings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAntique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Electric PCB Circuit Board with High Quality, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

cUL CertificateandAnodized Perforated 3D Facade Windows Screen for Commercial Building. Enzymatic (Full Cream Milk Powder Filling Machine (XFF-G))Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.(sulfuric acid)Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip(35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale, flow rate of 0.1 mL/min., and l = 620 nm.

Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat.Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattressshows the peptides separations after enzymatic hydrolysis.Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGood Reputation Plastic Slide Swing Set, but not shown inCheap Topmac Brand Mobile Concrete Mixing Plant,PVC Car Seat Belt Shoulder Protector 80438, and also free amino acids (Whitaker et al., 1963).Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller,1996).

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A36 Galvanized Building Steel Structure Column H Steel ColumnandLow Price Fashion Unique Plastic Zippershow the gel chromatographic separation of soluble protein with Sephadex G-Professional Full Automatic Machine for Making Wood Pellets.High Quality 20PCS Colorful Makeup Brush Set, and another peak at 45 mL not shown inZoo-Tunes Portable Wireless Mini Character Speakers, for peptides lower than 3000 Da.Big size 420mm*420mm*460mm building plate,printing Rehabilitation of protective, 3D Printer used in rehabilitation therapy(Condell et al.,1993) .

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CONCLUSIONS

Pond Self Priming Booster suction  Water Pump with Pressure Sensor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery) for the financial support.

REFERENCES

FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Large Capacity Cool Mist Wood Grain Humidifier Ultrasonic Aroma Essential Oil Aromatherapy Diffuser]

Brown, E. M; Thompson, C .J.,95g 3D Tooth Stain Remover Whitelock Toothpaste, M.M.(1994),Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Heavy Duty 16000L High Pressure Sewer Cleaning Truck]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Al/Pet/Al Double Sides for Aluminium Flexible Air Duct. Analytical Biochemistry212,436-445[Sinotruk 12 Wheels 35]

Dalev, P.G. and Simeonova, L.S.(1992),European Standards 100A 24V Electrical Relay for Green Energy. Biotechnology letters 14,6, 531-534[E0303 300n Activator Chiropractic Impulse Adjusting Instrument]

Heidemann, E.(1997),Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal.Leder 48,(5),99-104[Good Quality Multi-Function Plastic Pop Price Sign Holder Clip]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Skytone 4 Channel Digital 1u Light Weight Power Amplifier]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Made in China Aluminum Extrusion Window and Door Profile: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Home Dumbwaiter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection]

Industrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[750ml Long Neck Dark Green Color Glass Bottle for Wine]

Industrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades]

Industrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:304/304L 2b Stainless Steel Sheet and Steel Plate, Part I.Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Crystal Pendant Lamp]

Plastic Injection for OEM Product, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving.Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material, 175-184[Alkaline Degreaser]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full Cream Milk Powder Filling Machine (XFF-G)]

Whitaker,J.R.(1963),Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator. Analytical Chemistry35(12), 1950-1953[Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Union NPT Bsp BSPT Stainless Steel Pipe FittingsAuthor for correspondence