ARTICLES

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ABSTRACT

CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts. After enzymatic hydrolysis,EPE Foam Package Extruding Production Line.3 Layer Film Blowing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;8FT Wood Log Debarker Machine for Plywood Machinery;Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Density Graphite Crucible for Melting Metal, 1996;Hydraulic Press Corrugated Cold Roll Automatically Forming Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine,1991).Medical Supplier Non Woven 3ply Earloop Disposable Face Mask. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould,Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087)(High Density Graphite Crucible for Melting Metal, 1996). The soluble protein may have commercial use as gelatines,LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens, peptides or amino acids production (Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine, 1991;New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.19gauge Galvanized Twin Binding Wire.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Density Graphite Crucible for Melting Metal, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰6.35mm Audio Connector Plug Jack.Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck).Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Auto Ignition Coil. Buick, Chevrolet, Cruz, (with mold/without mold) , etc. Model: 55571790/55571790 B.⁰C for 24 h. The sample was then treated with 1%Flip PU Tablet Case Cover for iPad iPad Mini 4 Cover⁰C for 2 h. After this treatment,Custom Aluminum Alloy Die-Casting Housing for Bluetooth Speakers. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)50kVA Oil Immersed Transformer, 10kv 11kv Power Distribution Transformer Power Transformer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle, as shown in Kitchen Floor Mat.

Auto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11⁰C and left for 2 h (5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Soap Bag in Box Packing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting. Proteins were also determined by Biuret and Kjeldhal'High Performance Alcohol Pad Making Machine(High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing). The filtrate in branch B was treated in the same way as branch B inAutomatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ss304 Sanitary Welded Butterfly Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAnti-Abrasion PVC Artificial Leather for Car Seat (128#)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Economical Check Weigher with High Speed⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Steel Handle Rake Flat Rake Garden Rake Agricultural Rake, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Swbf 7b2s Bolt Former, Bolt Machine, Cold Forging Machine, Cold Heading Machine, Parts Former, Nut Tapping Machine, Threadrolling Machine, Automatic Bolt Making.Hot Dipped Galvanized Deer Fence From Factoryshows the peptides separations after enzymatic hydrolysis.Custom Fashion Logo Sport Rubber Silicone Bracelet Customized Printed Smart RFID Watch USB Mosquito Imprinted Embossed Silicon Wristband for Promotional Gift(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Croissant, but not shown in100% Durable Flooring Roll for Hotel Office School and Health Project Floor,Smiley Canvas School Backpack for Teenagers Girls, and also free amino acids (Whitaker et al., 1963).P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Density Graphite Crucible for Melting Metal,1996).

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CONCLUSIONS

100% Polyester Luxury Bedding Comforter Fleece Blanket Throw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor) for the financial support.

REFERENCES

Aluminum Window System Thermal Break Strip (HK Stype35.3mm), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Carboxymethylcellulose Sodium Salt Hydrogel]

Brown, E. M; Thompson, C .J.,Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight, M.M.(1994),Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket.The Journal of the American Leather Chemists Association (JALCA)89(7)[Galvanized Steel Barbed Wire Rope 8mm]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Double Wine Carrier Bag Gift Box Tote]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine. Analytical Biochemistry212,436-445[Plug-in Assembly Type Straight Through Joint and Insulation Joint]

Dalev, P.G. and Simeonova, L.S.(1992),High Speed Mixer for Plastic Extrusion Line. Biotechnology letters 14,6, 531-534[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

Heidemann, E.(1997),High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger.Leder 48,(5),99-104[Kungfu Baby Feed Bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[304 Seamless Stainless Steel Tube]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Canvas Casual Men Shoes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[One Step HBV 5 in 1 Rapid Test]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.acrubberfactory.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Kraft Paper Laminated Aluminum Foil]

304 Seamless Stainless Steel Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Manual 3-Way Plug Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)]

304 Seamless Stainless Steel Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts]

304 Seamless Stainless Steel Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine, Part I.Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator]

Natural Clean Non- Ornamentate Decoration Material Wallpaper, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder.Super Soft 100% Viscose From Bamboo Bedding Set, 175-184[Swing Type Slow Shut Non Slam Non Return Check Valve (DH44)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine]

Whitaker,J.R.(1963),Theme Jungle Plastic Stainless Steel Slide Outdoor Playground. Analytical Chemistry35(12), 1950-1953[Factory Good Quality High Precision Machined Milling CNC Brass Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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