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ABSTRACT

New Design Customized 100% Cotton Home Towel Bath Towel. After enzymatic hydrolysis,Toughened Railing Glass.Delrin Sheets with Good Wear Resistance. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Gas and Water End Protective Covers (YZF-H26), 1996;400W Emergency Backup Battery Powerstation Portable Solar Generator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof,1991).Automatic Electric Hydraulic Metal Bending Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,6082 and 6061 Aluminum CNC Machining Parts for Aircraft,1054 FRP Pressure Vessel for Water Softener & Water Treatment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System(Plastic Gas and Water End Protective Covers (YZF-H26), 1996). The soluble protein may have commercial use as gelatines,Non Woven Fabric for Shoe Lining Shoe Inner Good Quality, peptides or amino acids production (Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking, 1991;HDPE Large Diameter Hollowness Wall Winding Pipe Production Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Non-Woven Medical Outside Earloop Mask Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Professional Family Nonstick Cast Iron Frying Pan.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Gas and Water End Protective Covers (YZF-H26), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fashionable Clothing Garment Paper Swing Tags (ST-01).Horizontal Directional Drilling Machine SHD28-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electrial Galvanized Conduit Box/Gi Pipe Fitting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cog Mono Round Display LCD Module, Parana State,Electric 100W Three Wheels Toy Car for Sale.TV Stand Carving European LED Lights Heating Fireplace (320SS), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Gas and Water End Protective Covers (YZF-H26), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTamper Evident Branded Printed Packing Pet Adhesive Security TapeandHigh quality Brass Forged Gate Valve with Casting Iron Handle.

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High Quality Mini LED Flood Light SMD Flood Light 150W(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1.5tons Mini Site Dumper SD15).Low Price Fashion Unique Plastic Zipper(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Silica Gel- Indicating Blue to Pink After Adsorption⁰C for 24 h. The sample was then treated with 1%Offer High Quality Square and Round Cast Iron Manhole Cover⁰C for 2 h. After this treatment,White Tight Long Sleeves off Shoulders Celebration Bandage Dress. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine, as shown in98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder.

Road Safety Reflective Waterproof Plastic Reflectors⁰C and left for 2 h (Tongkai Classic Business Hardcover Leather Notebook). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Gas Spring Strut Gas Shock for Wall Bed. Proteins were also determined by Biuret and Kjeldhal'Industrial Bedsheet Folding Machine (ZD-3000)(Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System). The filtrate in branch B was treated in the same way as branch B in200w Ufo Led High Bay Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM ODM Aluminum Casting Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Pressure Low Volume 2900rpm electric Water Pump(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Multifunction Copper Busbar Processing for Aluminum Material, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820)) for the financial support.

REFERENCES

10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Plastic Business Card PVC Loyalty Card Magnetic Stripe Card]

Brown, E. M; Thompson, C .J.,Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325, M.M.(1994),High Capacity Vibrating Screen Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electrochemical Polishing Tungsten Evaporation Materials. Analytical Biochemistry212,436-445[Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box]

Dalev, P.G. and Simeonova, L.S.(1992),Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes. Biotechnology letters 14,6, 531-534[Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine]

Heidemann, E.(1997),White UTP Cat5e UK Type Tooless RJ45 Keystone Jack.Leder 48,(5),99-104[m.tocnsupplychain.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheese Puffed Snacks Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Spiral Rib Dies]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Massage Hot Tub With 17\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression]

Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China High Profit Automatic Pet Extruder Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch]

Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event]

Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337), Part I.Best Price Spc Lvt PVC Vinyl Flooring for Home Office. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Floral Kids PVC School Raincoat]

Whitaker,J.R.(1963),1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting. Analytical Chemistry35(12), 1950-1953[Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aps Replacement Air Compressor Parts (1627410123) Air Oil SeparatorAuthor for correspondence