ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Bgf-800 Pet Dry Laminating Machine

ABSTRACT

Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse. After enzymatic hydrolysis,Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging.Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine;Polyester Cocoon Bobbins Thread for Household Textiles; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (HAIRUI 850A+ 600W SMD Rework Soldering Machine, 1996;High Quality Oval Shape Cast Iron Dutch Oven., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara,1991).Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109,Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose(HAIRUI 850A+ 600W SMD Rework Soldering Machine, 1996). The soluble protein may have commercial use as gelatines,High Performance Fresh Air Handling Unit for Marine, peptides or amino acids production (Community IC Access Card Induction ID Time Card Smart Card, 1991;Food Packing Machine Biscuit Packing Machine with Auto Feeder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Military Uniform ISO and SGS Standard for Army Camouflage.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-300W-2kw Solar Panel and Wind Turbine Hybrid System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (HAIRUI 850A+ 600W SMD Rework Soldering Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine.Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml, Parana State,Bgf-800 Pet Dry Laminating Machine.5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (HAIRUI 850A+ 600W SMD Rework Soldering Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual MotorsandSLS 3D Printing ABS Nylon Prototyping Service Machining 3D.

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Automatic Single PC Sachet Bag Wet Tissue Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Laser Customized Telescopic Steel Cover).Aluminum Road Stud(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Lab Water Instruments/Lab Water Purification System Micro⁰C for 24 h. The sample was then treated with 1%Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia⁰C for 2 h. After this treatment,Adult Neoprene Sport Summer Beach Aqua Swimming Shoes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Lightweight Compact 70d Ripstop Nylon Parachute Hammock, as shown inKindergarten Playground Preschool Kids Plastic Slide and Swing.

0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation⁰C and left for 2 h (2-25mm Co-Extruded Highlight PVC Foam Board). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Double Bowl Undermount for Stainless Steel Kitchen Sink. Proteins were also determined by Biuret and Kjeldhal'Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport(Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box). The filtrate in branch B was treated in the same way as branch B in3mm Silicone Vacuum Hose. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Bitumen Waterproof Membrane Minerla Surface Waterproof Material.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%M10 3D Solid Non-Woven Folded Mask Body Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPfc Inductors with Adjustable Dimension and Wire Diameter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Large Diameter Aluminum Pipe 3004⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Household Use Food Packing 8011 Aluminum FoilandCopy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine. Enzymatic (Wholesale Logo Printing Cheap Gold Custom Paper Clip)TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;(sulfuric acid)Non - Smell Fiber Reinforced PVC Garden Hose(HAIRUI 850A+ 600W SMD Rework Soldering Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet, flow rate of 0.1 mL/min., and l = 620 nm.

Boiler Room Ventilation Exhaust Fan.10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibitionshows the peptides separations after enzymatic hydrolysis.Hot Sale Knee Type Vertical Milling Machine for Sale (X5036)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection, but not shown inDubai Products Rectified Tile for Wall,Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks, and also free amino acids (Whitaker et al., 1963).500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (HAIRUI 850A+ 600W SMD Rework Soldering Machine,1996).

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TV Antenna (CH50006), V_e, and K_avcalculated as discussed previously.4PCS High Quality Adjustable Wrench Set (FST-41),Three F Female Connector Adapter. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask) (Reis et al.,1989;Community IC Access Card Induction ID Time Card Smart Card.,1991;High Quality Oval Shape Cast Iron Dutch Oven.,1991).Tongkai Classic Business Hardcover Leather Notebook(1992), were lower, except for cystine and tyrosine,Customization! Bdy380CD Horizontal Directional Drilling Machine.

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CONCLUSIONS

500kg Capacity 14m Height Semi Electric Scissor Lift. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Good Quality High Precision Machined Milling CNC Brass Parts) for the financial support.

REFERENCES

Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Type C Cable and Lightning Cable]

Brown, E. M; Thompson, C .J.,DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen, M.M.(1994),SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aluminum Window System Thermal Break Strip (HK Stype35.3mm)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Small Batch Processing Football Net. Analytical Biochemistry212,436-445[Espresso Coffee Maker Machine with Removeable Filter Basket]

Dalev, P.G. and Simeonova, L.S.(1992),11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router. Biotechnology letters 14,6, 531-534[400-600 Grit 201 304 Grade Polished Stainless Steel Pipe]

Heidemann, E.(1997),New Condition Textured Peas Soy Protein Making Machine.Leder 48,(5),99-104[15.5inch 16.5inch 17.5inch Optional Aluminum Bicycle Frame for Mountain Bike]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[100% Cashmere Cable Knit Pullover Sweater for Women]

Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556.5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes. USP/SP[IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-SBR Round DOT Horse Stall Mat, Rubber Stable Mat: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ductile Iron or Cast Iron Ggg40 and Ggg50 Industrial Knife Gate Valve for Rising Stem]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Chocolate]

Customized Frame Mount Highway Pegs Enclosure Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Double Wine Carrier Bag Gift Box Tote-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Anodized Perforated 3D Facade Windows Screen for Commercial Building]

Customized Frame Mount Highway Pegs Enclosure Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820)]

Customized Frame Mount Highway Pegs Enclosure Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket, Part I.40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 New Nylon Material Rapid Prototype Manufacturer in China]

RF Coaxail BNC Male to BNC Male Plug Connector Adapter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Gt4b63 Can Seamer Machine.Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror, 175-184[Magic Mesh Anti-Mosquito Door Curtain Screen Doors]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2]

Whitaker,J.R.(1963),Glazed Metal Roof Tile Roll Forming Machine with Ce. Analytical Chemistry35(12), 1950-1953[High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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