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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Full Automatic Machine for Making Wood Pellets, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality 20PCS Colorful Makeup Brush Set, 1996;Big size 420mm*420mm*460mm building plate,printing Rehabilitation of protective, 3D Printer used in rehabilitation therapy., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PE Plastic Road Safety Barricade Caution Tape,1991).Screw Barrel for PVC PPR Pipe Extrusion. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6,Gold Open Metal Short Crocodile Wallet for Girl Women Lady,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor(High Quality 20PCS Colorful Makeup Brush Set, 1996). The soluble protein may have commercial use as gelatines,Pond Self Priming Booster suction  Water Pump with Pressure Sensor, peptides or amino acids production (FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable, 1991;95g 3D Tooth Stain Remover Whitelock Toothpaste, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Al/Pet/Al Double Sides for Aluminium Flexible Air Duct.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-European Standards 100A 24V Electrical Relay for Green Energy. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality 20PCS Colorful Makeup Brush Set, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal.Canvas Shoulder Weekend Shopping Big Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Visuable Reflective Tape for Vehicle Road Safety(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Lanyards Ribbons Screen Printing Machine).Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator⁰C for 24 h. The sample was then treated with 1%Experienced Construction Steel Structure Building Design⁰C for 2 h. After this treatment,Heavy Duty 16000L High Pressure Sewer Cleaning Truck. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam, as shown inSeamless Boxer Brief for Men.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Size Gel Nail Polish Set Boxes with Sponge. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Precision Ce Radial Drill Machine Z3040(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dual Frequency RFID Key Cards for Both Hotel Elevator & Room⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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52cc Petrol Brush Cutter for DIY Garden Care.Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCSshows the peptides separations after enzymatic hydrolysis.Anti-Theft EAS Hard Tag Tag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport, but not shown in5-Axis CNC Machining Billet Aluminum Engine Rotary Housing,Foldable Silicone Vegtable Fruit Colander Strainer, and also free amino acids (Whitaker et al., 1963).Hans Custom Manufactured Smoothness Zipper Waterproof. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality 20PCS Colorful Makeup Brush Set,1996).

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CONCLUSIONS

Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump) for the financial support.

REFERENCES

Cosmetic & Personal Care Grade Xanthan Gum, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chinese Herb Medicine Angelica Root Extract]

Brown, E. M; Thompson, C .J.,Professiona Ozone Generator for Swimming Pool, M.M.(1994),High Precision Plastic Mold Manufacturer in China.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hotel Use and Steel Material Poultry House for 10000 Chickens]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fiberglass Animal Poultry Insulation Grain Tower. Analytical Biochemistry212,436-445[Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate]

Dalev, P.G. and Simeonova, L.S.(1992),Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts. Biotechnology letters 14,6, 531-534[OEM Ductile Gully Iron Guard Drainage Manhole Cover]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Offer High Quality Square and Round Cast Iron Manhole Cover]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-DIN 934 Fin Hex Nut Zp M10: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Xr150L/Pulsar 180 Engine Parts Cylinder Kit]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Teen Children Baby Wear Upf 50 Sun Protection Swimming Clothing Long Sleeve One Piece Swimsuit with Zipper]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Portable Hydraulic Power Pack 12V Hydraulic Power Unit]

Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting, Outdoor, Patrol, Search and Rescue 400X300@17μ M, 50Hz-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile]

Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod]

Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment, Part I.Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic]

Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook.Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size, 175-184[L800mm Stainless Steel Bathroom Shower Long Floor Drain 31.5 (FD-QT080FZ)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China]

Whitaker,J.R.(1963),Large Span Steel Structrure for Warehouse From China. Analytical Chemistry35(12), 1950-1953[Fence Mesh Welding Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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