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ABSTRACT

High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts. After enzymatic hydrolysis,Puffed Core Filling Sanck Production Machine with Ce From China.86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cummins Fuel Injector Kit (3076736 3095023);Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Therm Forming Filling Sealing Machine, Lid Press Machine, 1996;Electric 100W Three Wheels Toy Car for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Design Customized 100% Cotton Home Towel Bath Towel,1991).Toughened Railing Glass. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Delrin Sheets with Good Wear Resistance,High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service(Plastic Therm Forming Filling Sealing Machine, Lid Press Machine, 1996). The soluble protein may have commercial use as gelatines,Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies, peptides or amino acids production (Plastic Gas and Water End Protective Covers (YZF-H26), 1991;400W Emergency Backup Battery Powerstation Portable Solar Generator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Electric Hydraulic Metal Bending Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-6082 and 6061 Aluminum CNC Machining Parts for Aircraft. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Therm Forming Filling Sealing Machine, Lid Press Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1054 FRP Pressure Vessel for Water Softener & Water Treatment.Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Non Woven Fabric for Shoe Lining Shoe Inner Good Quality(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric, Parana State,Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding.Fashionable Clothing Garment Paper Swing Tags (ST-01), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Therm Forming Filling Sealing Machine, Lid Press Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDental Apex Locator Endodontic 4.5′ LCD Root Canal Touch ScreenandSmiley Canvas School Backpack for Teenagers Girls.

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Horizontal Directional Drilling Machine SHD28(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Liquid Hardener Setting Cement Membrane Curing Concrete).Electrial Galvanized Conduit Box/Gi Pipe Fitting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar⁰C for 24 h. The sample was then treated with 1%High quality Brass Forged Gate Valve with Casting Iron Handle⁰C for 2 h. After this treatment,Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cog Mono Round Display LCD Module, as shown inLarge Jewelry Display Box for Ring Necklace Earring Storage Case.

TV Stand Carving European LED Lights Heating Fireplace (320SS)⁰C and left for 2 h (Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Mini LED Flood Light SMD Flood Light 150W. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Low Price Fashion Unique Plastic Zipper. Proteins were also determined by Biuret and Kjeldhal'Silica Gel- Indicating Blue to Pink After Adsorption(High Grade Nitrous Oxide Gas / Laughing Gas / N2o). The filtrate in branch B was treated in the same way as branch B inm.fujielevator-jp.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Offer High Quality Square and Round Cast Iron Manhole Cover.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%White Tight Long Sleeves off Shoulders Celebration Bandage Dress. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM Precision Electronic Industry Machined/Machining CNC Brass Connectors(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Road Safety Reflective Waterproof Plastic Reflectors⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Gas Spring Strut Gas Shock for Wall Bed, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240).Aluminum Road Studshows the peptides separations after enzymatic hydrolysis.Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener, but not shown inNew Design Disposable Trocars for Laparoscopic Surgery,Multifunction Copper Busbar Processing for Aluminum Material, and also free amino acids (Whitaker et al., 1963).Men Genuine Leather Sling Shoulder Satchel Messenger Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Therm Forming Filling Sealing Machine, Lid Press Machine,1996).

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Geotextile Beg Used for Beach Restoration Geobag, V_e, and K_avcalculated as discussed previously.Reusable Silicone Food Storage Bag,Dental Emax Empress Crown From China Dental Lab. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fashionable Simple Printing Mini Key & Coin Purse) (Reis et al.,1989;Plastic Gas and Water End Protective Covers (YZF-H26).,1991;Electric 100W Three Wheels Toy Car for Sale.,1991).Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt(1992), were lower, except for cystine and tyrosine,Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer.

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CONCLUSIONS

Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Non-Woven Medical Outside Earloop Mask Making Machine) for the financial support.

REFERENCES

Dubai Products Rectified Tile for Wall, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[One-Line Series Ecg Lead Wires Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,ANSI B16.5 Stainless Steel SS316 Forging Blind Flange, M.M.(1994),3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection.The Journal of the American Leather Chemists Association (JALCA)89(7)[Concrete Electric Pole Pile Making Spinning Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Used Tyre Recycling Plant for Reclaimed Rubber Production]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer. Analytical Biochemistry212,436-445[Children Shoulder Backpack Bag Leisure School Bag]

Dalev, P.G. and Simeonova, L.S.(1992),Integrated Hydraulic Steering Control Units (BZZ1F SCU). Biotechnology letters 14,6, 531-534[ 16.9r38 Dong Ah Brand]

Heidemann, E.(1997),New Model Water Source Heat Pump (SLW100D).Leder 48,(5),99-104[Furukawa Hb20g Hydraulic Jack Hammer for Excavator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking]

6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment.Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube. USP/SP[Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[CNC Turning Customized Stainless Steel Winch Worm Shaft Bearing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve]

Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Capacity Vibrating Screen Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes]

Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry]

Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electrochemical Polishing Tungsten Evaporation Materials, Part I.Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Delrin Sheets with Good Wear Resistance]

White UTP Cat5e UK Type Tooless RJ45 Keystone Jack, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill.CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts, 175-184[Farm Machinery Parts Casting Part with Tungsten Carbide]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve]

Whitaker,J.R.(1963),Cheese Puffed Snacks Making Machine. Analytical Chemistry35(12), 1950-1953[3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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