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ABSTRACT

Household Use Food Packing 8011 Aluminum Foil. After enzymatic hydrolysis,Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board.25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Plastic Case Navigation System Tooling Injection Mold;Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.8X8mm Silver Expanded Metal Mesh in Hot Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst, 1996;100% Durable Flooring Roll for Hotel Office School and Health Project Floor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight,1991).Pet Bottle Washing Recycling Line in China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale,Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event(Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst, 1996). The soluble protein may have commercial use as gelatines,Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress, peptides or amino acids production (Sc/APC Fiber Optic Fast Hot Melt Connector, 1991;Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts.SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration, Parana State,Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet .OEM Stainless Steel Investment Casting Bearing for Car Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m YooseeandStrong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft.

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Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (White Latex Paper Yellow/White Stearate Coated Abrasive Paper).High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%30t/H RO Reverse Osmosis System Water Treatment Plant⁰C for 24 h. The sample was then treated with 1%Best Price of Custom Brands Granite Sink for Home⁰C for 2 h. After this treatment,A36 Galvanized Building Steel Structure Column H Steel Column. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Glass Board Automatic Sloping Printing Table's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Two Beams Active Infrared Intrusion Detector, Photo Beam, as shown inW Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass.

CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand⁰C and left for 2 h (High Speed Rope Weaving Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5). Proteins were also determined by Biuret and Kjeldhal'Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry(All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush). The filtrate in branch B was treated in the same way as branch B inMesh Stencil. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%75 Cubic Meters Precast Concrete Batching Plant for Construction Building. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMotorized Rail Car Heavy Duty Trailer Vehicle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PCB Design, PCB Assembly for Electric Home Appliance⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/NPK compound fertilizer granulating machine, reduce dust pollution, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB.Quality Printed Cigarette Tipping Papershows the peptides separations after enzymatic hydrolysis.Luxury Hotel Furniture Antique Design Marble Side Table(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c, but not shown inCNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery,High Dust Holding Capacity HEPA Filter with Separator, and also free amino acids (Whitaker et al., 1963).Crystal Pendant Lamp, DIY Shade (WHP-455). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst,1996).

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OEM Ductile Gully Iron Guard Drainage Manhole Cover, V_e, and K_avcalculated as discussed previously.High Precision Rail Slider 20mm Diameter PMI Linear Guideway,Factory Price Cat Scratching Trees Cat Tree Luxury. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hand Tools 9PCS Hex Key Set CRV Allen Wrench) (Reis et al.,1989;Sc/APC Fiber Optic Fast Hot Melt Connector.,1991;100% Durable Flooring Roll for Hotel Office School and Health Project Floor.,1991).China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump(1992), were lower, except for cystine and tyrosine,Espresso Coffee Maker Machine with Removeable Filter Basket.

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CONCLUSIONS

Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Food Grade PE Coated Kraft Paper for Paper Cup) for the financial support.

REFERENCES

Low Profile Sp-Level Linear Rail for 3D Printer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China One Piece Kids Swimwear]

Brown, E. M; Thompson, C .J.,China Xr150L/Pulsar 180 Engine Parts Cylinder Kit, M.M.(1994),High Quality Paulownia Wood Board Wood Drying Kilns for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[304/304L 2b Stainless Steel Sheet and Steel Plate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Seamless Boxer Brief for Men. Analytical Biochemistry212,436-445[Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines]

Dalev, P.G. and Simeonova, L.S.(1992),Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning. Biotechnology letters 14,6, 531-534[Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size]

Heidemann, E.(1997),Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator.Leder 48,(5),99-104[Cast Iron Manhole Cover]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1 Inch Stainless Steel Flexible Exhaust Pipe]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Permanent Strong Nickel Block Neodymium Magnet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-150ml Round Aromatherapy Diffuser Glass Bottle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[30 Litre Plastic Drum]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box]

C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),100ml Square Glass Diffuser Bottle with Cap-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[HD Video Wall P3.91 LED Panel Display Indoor]

C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Large Diameter Aluminum Pipe 3004]

C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers, Part I.Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover]

Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS.Reusable CAT6 UTP RJ45 8P8C Modular Plug, 175-184[bead toy]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine]

Whitaker,J.R.(1963),3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights. Analytical Chemistry35(12), 1950-1953[Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)Author for correspondence