ARTICLES

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ABSTRACT

Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking. After enzymatic hydrolysis,HDPE Large Diameter Hollowness Wall Winding Pipe Production Line.Non-Woven Medical Outside Earloop Mask Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fashionable Clothing Garment Paper Swing Tags (ST-01), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Horizontal Directional Drilling Machine SHD28, 1996;Electrial Galvanized Conduit Box/Gi Pipe Fitting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar,1991).High quality Brass Forged Gate Valve with Casting Iron Handle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820),High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cog Mono Round Display LCD Module(Horizontal Directional Drilling Machine SHD28, 1996). The soluble protein may have commercial use as gelatines,TV Stand Carving European LED Lights Heating Fireplace (320SS), peptides or amino acids production (High Quality Mini LED Flood Light SMD Flood Light 150W, 1991;Low Price Fashion Unique Plastic Zipper, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Silica Gel- Indicating Blue to Pink After Adsorption., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Offer High Quality Square and Round Cast Iron Manhole Cover.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-White Tight Long Sleeves off Shoulders Celebration Bandage Dress. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Horizontal Directional Drilling Machine SHD28, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors.4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Road Safety Reflective Waterproof Plastic Reflectors(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

OEM ODM Aluminum Casting Parts, Parana State,Non Woven Fabric for Shoe Lining Shoe Inner Good Quality.High Pressure Low Volume 2900rpm electric Water Pump, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Horizontal Directional Drilling Machine SHD28, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowland IV Fluid Set with Precision Filter & Luer Lock.

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New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Logo Printing Cheap Gold Custom Paper Clip).Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch⁰C for 24 h. The sample was then treated with 1%Multifunction Copper Busbar Processing for Aluminum Material⁰C for 2 h. After this treatment,Men Genuine Leather Sling Shoulder Satchel Messenger Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electric DC 24V Geared Motor for Auto Doors's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided, as shown inEPE Foam Package Extruding Production Line.

RF Shielding MRI Door with Brass Frame for RF Cage⁰C and left for 2 h (PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Geotextile Beg Used for Beach Restoration Geobag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Reusable Silicone Food Storage Bag. Proteins were also determined by Biuret and Kjeldhal'Dental Emax Empress Crown From China Dental Lab(Outdoor P5mm Programmable Bus LED Display Sign). The filtrate in branch B was treated in the same way as branch B inCheap Oxygen Concentrator. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEnig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ANSI B16.5 Stainless Steel SS316 Forging Blind Flange⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Spirits

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Cheese Puffed Snacks Making Machine, V_e, and K_avcalculated as discussed previously.Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration,China High Profit Automatic Pet Extruder Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm)) (Reis et al.,1989;High Quality Mini LED Flood Light SMD Flood Light 150W.,1991;Electrial Galvanized Conduit Box/Gi Pipe Fitting.,1991).Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337)(1992), were lower, except for cystine and tyrosine,Best Price Spc Lvt PVC Vinyl Flooring for Home Office.

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CONCLUSIONS

Red Papillon (JSY-3) Satin Ribbon Flower. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Industrial Bedsheet Folding Machine (ZD-3000)) for the financial support.

REFERENCES

Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Basic Blue 9 Suppliers for Sale]

Brown, E. M; Thompson, C .J.,Steel Structure Construction Building Metal House, M.M.(1994),1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Professional HD IPS 10.1 LCD Monitor with SDI]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera. Analytical Biochemistry212,436-445[Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11)]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Sanitary Thread Pneumatic Butterfly Valve. Biotechnology letters 14,6, 531-534[PP Base Packaging Film Pet/PE Laminating Food Packaging Film]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Computer Screen etc]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carboxymethylcellulose Sodium Food Grade]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output]

Hot Sale Energy Saving Induction Heater for Billet Making, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electric 154 Stone Polisher 17inch Floor Cleaning Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Three F Female Connector Adapter]

Hot Sale Energy Saving Induction Heater for Billet Making,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder]

Hot Sale Energy Saving Induction Heater for Billet Making, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic, Part I.2019 New Nylon Material Rapid Prototype Manufacturer in China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Recycle Finger Joint Core Film Faced Shuttering Plywood]

Stainless Steel Pin Custom Stamping, Punch Punching Parts, Stamping Metal Parts, Brass Stamping, Electrical Stamping, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018.CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101, 175-184[Stainless Steel Tower Bolt Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hydraulic Press Corrugated Cold Roll Automatically Forming Machine]

Whitaker,J.R.(1963),High Quality Portable Polyester Duffel Garment Tote Bag. Analytical Chemistry35(12), 1950-1953[FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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