ARTICLES

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ABSTRACT

High Grade Nitrous Oxide Gas / Laughing Gas / N2o. After enzymatic hydrolysis,Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor.Easy Soak off OEM 10ml Gel Nail Polish. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cosmetic Case Box Packaging Makeup Case for Outdoor;Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Foil Container Making Machine, Pneumatic Punching Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED Pixel Light U4 1.2W, 1996;Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (15L/30L Vibratory Deburring Polishing Tumbler for Small Parts,1991).10/16A EV Potable Charging Station Type 2 621962 Plug Cee. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794,Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED(LED Pixel Light U4 1.2W, 1996). The soluble protein may have commercial use as gelatines,Low Noise Ventilating Blower Fan for Agriculture, peptides or amino acids production (Waterproof Chipboard, Melamine Chipboard for Furniture, 1991;Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Foshan Terracotta Facade Panel for Commercial Building Curtain Wall. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED Pixel Light U4 1.2W, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set.Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Uic Standard Railway Type Y25 Bogie for Different Freight Wagon(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps, Parana State,ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross.Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (LED Pixel Light U4 1.2W, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming MachineandBlince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor.

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Ball Mill, Grinding Mill, Mining Equipment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting).Used Tyre Recycling Plant for Reclaimed Rubber Production(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c⁰C for 24 h. The sample was then treated with 1%Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head⁰C for 2 h. After this treatment,Medical Ce Approved Bp Machine Blood Pressure Monitor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery, as shown inPhysical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment.

Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish⁰C and left for 2 h ( Vodka). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Jieshuai 3 in 1 USB Cable, Fast Charging Data Cable, Universal Micro USB Cable, Lightning Cable, Type C Cable, Suitable Apple iPhone and Android Charge. Proteins were also determined by Biuret and Kjeldhal'Mini Electric Chain Saw Cutting Machine Price(Plastic Nylon Sheet with High Temperature). The filtrate in branch B was treated in the same way as branch B inStainless Seamless Steel Tubes High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,A2 Grade Fireproof Customized Color ACP Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ce Approved Cheap Medical Disposable Electrical Infusion Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Party Decoration Classic Wine Glass Accessories Jewelry(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Galvanized Steel Barbed Wire Rope 8mm, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CNC Aluminum Laser Milling Machinery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Horizontal Heavy Duty High Pressure Multistage Centrifugal Water PumpandHigh Porosity Zirconia Zro2 Foam Filter for Foundry. Enzymatic (Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression)Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops(sulfuric acid)Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids(LED Pixel Light U4 1.2W, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844), flow rate of 0.1 mL/min., and l = 620 nm.

Antique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair.High Lumen Outdoor IP65 100W LED Flood Lightshows the peptides separations after enzymatic hydrolysis.Single Block Vertical Wire Drawing Machine for Screw and Bolt Making(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector, but not shown inRapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould,High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher, and also free amino acids (Whitaker et al., 1963).China Electric PCB Circuit Board with High Quality. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (LED Pixel Light U4 1.2W,1996).

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Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards., V_e, and K_avcalculated as discussed previously.Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip,High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6) (Reis et al.,1989;Waterproof Chipboard, Melamine Chipboard for Furniture.,1991;Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing.,1991).Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat(1992), were lower, except for cystine and tyrosine,Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment.

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CONCLUSIONS

PVC Car Seat Belt Shoulder Protector 80438. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Enamelled Heavy Duty Cast Iron Skillet) for the financial support.

REFERENCES

Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[72 Wells 1.5ml Centrifuge Tube Box]

Brown, E. M; Thompson, C .J.,Professional Full Automatic Machine for Making Wood Pellets, M.M.(1994),High Quality 20PCS Colorful Makeup Brush Set.The Journal of the American Leather Chemists Association (JALCA)89(7)[Foshan Terracotta Facade Panel for Commercial Building Curtain Wall]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Virgin Teflon PTFE Teflon Gaskets/Back up Rings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Big size 420mm*420mm*460mm building plate,printing Rehabilitation of protective, 3D Printer used in rehabilitation therapy. Analytical Biochemistry212,436-445[One to One Order Following Strong Real Gold Thread]

Dalev, P.G. and Simeonova, L.S.(1992),PE Plastic Road Safety Barricade Caution Tape. Biotechnology letters 14,6, 531-534[ Video Camera]

Heidemann, E.(1997),Screw Barrel for PVC PPR Pipe Extrusion.Leder 48,(5),99-104[Automatic Float Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[A2 Grade Fireproof Customized Color ACP Sheet]

CNC Turned Aluminum Anodized Auto Part, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6.Gold Open Metal Short Crocodile Wallet for Girl Women Lady. USP/SP[Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Embossed Pattern PU Leather Classic Mens Wallet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.plus1motors.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Custom Golden Colored Powder Pill Organizer]

Pond Self Priming Booster suction  Water Pump with Pressure Sensor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Auto/Manual Spray Painting Booth/Cabinet with Best Price]

Pond Self Priming Booster suction  Water Pump with Pressure Sensor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part]

Pond Self Priming Booster suction  Water Pump with Pressure Sensor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:95g 3D Tooth Stain Remover Whitelock Toothpaste, Part I.Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer]

Al/Pet/Al Double Sides for Aluminium Flexible Air Duct, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),European Standards 100A 24V Electrical Relay for Green Energy.Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal, 175-184[growook.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Irregular Shape Hologram Label Printing]

Whitaker,J.R.(1963),Canvas Shoulder Weekend Shopping Big Bag. Analytical Chemistry35(12), 1950-1953[1/4 25FT Twin Line Welding Hose for Oxy-Acetylene]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Medical Grade Ventilator for Sleep Apnea Breathing MachineAuthor for correspondence