ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Lab Water Instruments/Lab Water Purification System Micro. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia

ABSTRACT

Adult Neoprene Sport Summer Beach Aqua Swimming Shoes. After enzymatic hydrolysis,Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig.Lightweight Compact 70d Ripstop Nylon Parachute Hammock. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation;China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Double Bowl Undermount for Stainless Steel Kitchen Sink, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport, 1996;Bitumen Waterproof Membrane Minerla Surface Waterproof Material., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (M10 3D Solid Non-Woven Folded Mask Body Making Machine,1991).Pfc Inductors with Adjustable Dimension and Wire Diameter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Permanent Magnetic Lifter,Large Diameter Aluminum Pipe 3004,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light(Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport, 1996). The soluble protein may have commercial use as gelatines,Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater, peptides or amino acids production (Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine, 1991;Wholesale Logo Printing Cheap Gold Custom Paper Clip, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Non - Smell Fiber Reinforced PVC Garden Hose.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Boiler Room Ventilation Exhaust Fan.Hot Sale Knee Type Vertical Milling Machine for Sale (X5036)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

TV Antenna (CH50006), Parana State,Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia.4PCS High Quality Adjustable Wrench Set (FST-41), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg ChinaandFake Fish Luminescent Tropical Fish Fish Tank Decoration.

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Three F Female Connector Adapter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing).Tongkai Classic Business Hardcover Leather Notebook(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customization! Bdy380CD Horizontal Directional Drilling Machine⁰C for 24 h. The sample was then treated with 1%500kg Capacity 14m Height Semi Electric Scissor Lift⁰C for 2 h. After this treatment,Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose, as shown inZircon Butterfly Open Ring Diamond Fashion Woemn Rings.

Small Batch Processing Football Net⁰C and left for 2 h (Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Condition Textured Peas Soy Protein Making Machine. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556(Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line). The filtrate in branch B was treated in the same way as branch B inm.dgls-tech.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SBR Round DOT Horse Stall Mat, Rubber Stable Mat. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Frame Mount Highway Pegs Enclosure Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls, pH 3.25., mobile phase B: buffer solution of 0.01 mol/RF Coaxail BNC Male to BNC Male Plug Connector Adapter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Anti-Theft EAS Hard Tag TagandGt4b63 Can Seamer Machine. Enzymatic (Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror)Glazed Metal Roof Tile Roll Forming Machine with Ce(sulfuric acid)Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine(Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703), flow rate of 0.1 mL/min., and l = 620 nm.

Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment.Aluminum Waterproof Folding Marquee Canopy Quick up Tentshows the peptides separations after enzymatic hydrolysis.Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOriginal SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z, but not shown inAmusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale,Extruder Floating/Sinking Fish Feed Pellet Dryer, and also free amino acids (Whitaker et al., 1963).Custom Durable Silicone Rubber Seal Ring for Pressure Cooker. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport,1996).

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CONCLUSIONS

Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain) for the financial support.

REFERENCES

Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[13x23x61 Linear Bearings Factory OEM/ODM Product]

Brown, E. M; Thompson, C .J.,SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use, M.M.(1994),99% Al2O3 High Alumina Ceramic Ball.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Design Customized 100% Cotton Home Towel Bath Towel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mini Outdoor Hydraulic Man Aluminum Lift]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Price Long Life Portable Peltier Air Cooler. Analytical Biochemistry212,436-445[16oz Square Shape Colors Glass Mason Jar with Embossed Design]

Dalev, P.G. and Simeonova, L.S.(1992),8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club. Biotechnology letters 14,6, 531-534[High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture]

Heidemann, E.(1997),Hand Tools 9PCS Hex Key Set CRV Allen Wrench.Leder 48,(5),99-104[Mini Airbrush Kit For Makeup]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Private Customized High Quality Printed Shipping Labels]

Aluminum Road Stud, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover.Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply. USP/SP[Good Quality CZ Purlin One Machine Roll Forming Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rubber Seal for Crank Box of Hks Warp Knitting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Handheld Andriod POS with Thermal Printer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Premium Baby Toddler Food Feeding Silicone Bowl with Lid]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[DIN934 Zp Hex. Nut]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Density Graphite Crucible for Melting Metal]

Stainless Steel French Earring Hook for Jewelry, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Professional Metal Stamping Parts with Anodizing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Long Stroke Hydraulic Cylinder for Constraction Machine]

Stainless Steel French Earring Hook for Jewelry,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper]

Stainless Steel French Earring Hook for Jewelry, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Agriculture Indoor Vegetables and Fruits Planting Greenhouse, Part I.Night Vision Wide HD Waterproof Car Side View Camera for Bus. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst]

Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter.E2000/APC Fibre Optic Leads and Cables, 175-184[LED UV Ink for Offset Printing Machine and Relief Printing Machine Common Paper and PP Pet PVC Film]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25]

Whitaker,J.R.(1963),Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable. Analytical Chemistry35(12), 1950-1953[Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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