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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Stainless Steel LED Underground Light, Ground Lighting IP66


ABSTRACT

GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets). After enzymatic hydrolysis,K Series Helical Bevel Reduction Gearbox Motor.OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts;Mini Outdoor Hydraulic Man Aluminum Lift; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Fashion Women Clothes Fashion Yaga Pants, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride, 1996;Economic Full Automatic LED Pick and Place Machine for LED Strip Making., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (1/4 25FT Twin Line Welding Hose for Oxy-Acetylene,1991).PETG Acrylic Sheet for Window. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Frequency Online UPS W/3W-20K,Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output(High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride, 1996). The soluble protein may have commercial use as gelatines,14 Head Multihead Weigher for Pork with Screw Feeding, peptides or amino acids production (Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve, 1991;PSF 1000 Flat Plate Upper Discharge separator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Powerful Neodymium Pot Magnet Rare Earth Pot Magnet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC Gun Drilling Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products.High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Large Jewelry Display Box for Ring Necklace Earring Storage Case(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, Parana State,Stainless Steel LED Underground Light, Ground Lighting IP66.Supermarket Combined Island Chest Freezer Commercial Refrigerator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHexagonal Netting/ Gabion Box/ Gabion BasketandCustomization! Bdy380CD Horizontal Directional Drilling Machine.

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PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC).High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Clip on Wire Shelving Data Strip0C for 24 h. The sample was then treated with 1%Custom Black and White Hard Laptop Case Travel Suitcase0C for 2 h. After this treatment,Concrete Road Crack-Pouring Machine for Construction. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China OEM Alloy Steel Precision Casting, as shown inPopular Cheap Custom Logo Black Silicone Wristband in High Quality.

Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport0C and left for 2 h (Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.China Best Quality Hose Crimping Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor. Proteins were also determined by Biuret and Kjeldhal'Galvanized Metal Welded PVC Coated Wire Mesh Fence(Plastic Gas and Water End Protective Covers (YZF-H26)). The filtrate in branch B was treated in the same way as branch B inDad Baseball Hat And Cap. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavLuzhong-700 70HP 2WD Two Wheel Drive Farm Tractor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Top Quality Sanitary Tube Shell Tube Heat Exchanger0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Best Quality for Stretch Film Jumbo RollandHigh Precision iPhone Cases Fiber Laser Marking Machine. Enzymatic (Auto Water Cooling Radiator Release Temperature Screw Air Compressor)Injection Mold Maker for Plastic Fighter Toy(sulfuric acid)PCBA SMT Equipment PCB Depaneling Machine(High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%HDPE Pipe for Water Supply (OD: 16mm-800mm), flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%HDPE Pipe for Water Supply (OD: 16mm-800mm), flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker) for the financial support.

REFERENCES

Nylon Hammer Fixings Drive Anchor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush]

Brown, E. M; Thompson, C .J.,OEM Electrolyte Effervescent Tablet, M.M.(1994),Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh.The Journal of the American Leather Chemists Association (JALCA)89(7)[Powerful Neodymium Pot Magnet Rare Earth Pot Magnet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ridge Cap Panel Forming Machine Ridge Cap Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter. Analytical Biochemistry212,436-445[3 Layer Film Blowing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet. Biotechnology letters 14,6, 531-534[Aluminum Plate Fin Heat Exchanger/Oil Cooler Core]

Heidemann, E.(1997),20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant.Leder 48,(5),99-104[www.nbhonghuan.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electrial Galvanized Conduit Box/Gi Pipe Fitting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Manual Electric Winch Small Winch 220V Winch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Shacman 6x4 385hp 420hp 25ton Dumper]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07]

250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Two Wheel Foldable Electric Kick Scooter]

250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle]

250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A, Part I.Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c]

Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum Plate Fin Heat Exchanger/Oil Cooler Core.3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection, 175-184[Ye3 Ce CCC Certificated Tefc IC411 0.12~375kw Three Phase Induction AC IEC 60034-1 Electric Motor Duty Cycles S1 S2 S3 S4 for Fan Pump Blower Ye3-315L-2 185kw]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Drum Type Magnetic Separator]

Whitaker,J.R.(1963),3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet. Analytical Chemistry35(12), 1950-1953[PSF 1000 Flat Plate Upper Discharge separator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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