ARTICLES

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ABSTRACT

65 Polyester 35 Cotton Fabric Camouflage Printed Fabric. After enzymatic hydrolysis,Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine.Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve, 1996;FUJI Small Home Lift for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier,1991).Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ce Jacket Heated Water Chocolate Holding Tank Manufacturer,Lanyards Ribbons Screen Printing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Factory New-Product-Best-Quality-Customized-Metal-Pressing(China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve, 1996). The soluble protein may have commercial use as gelatines,Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine, peptides or amino acids production (Recycle Finger Joint Core Film Faced Shuttering Plywood, 1991;Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Beer Made in China OEM Abv 8.0%Vol Lager Beer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cat Cardboard House Pet Product Accessories.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-EPDM Crossfit Gym Mat/Rubber Interlocking Flooring. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor.Hf Vacuum Dryer Machine for Drying Wood-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (10mm Gold Plated Stainless Steel Balls G100 Polish Ball).400-600 Grit 201 304 Grade Polished Stainless Steel Pipe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply⁰C for 24 h. The sample was then treated with 1%Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables⁰C for 2 h. After this treatment,5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model, as shown inHot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box.

Car Interior Accessories Customized Car Seat Cover⁰C and left for 2 h (Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition. Proteins were also determined by Biuret and Kjeldhal'Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet(TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;). The filtrate in branch B was treated in the same way as branch B inSemi Automatic Pet Jar / Big 5L Bottle Blow Molding Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM/ODM Raw Material MDF HDF Laminate Door. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av7628 Fiberglass Cloth/Fabric(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.750ml Long Neck Dark Green Color Glass Bottle for Wine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Floral Kids PVC School Raincoat, pH 3.25., mobile phase B: buffer solution of 0.01 mol/MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making.50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inchshows the peptides separations after enzymatic hydrolysis.Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSs304 Sanitary Welded Butterfly Valve, but not shown in500kg Capacity 14m Height Semi Electric Scissor Lift,High Temperature & IR Drying Electronic Oven / Furnace, and also free amino acids (Whitaker et al., 1963).Ce Approved Bowrider Leisure Boat for 6-8 People. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve,1996).

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Concrete Electric Pole Pile Making Spinning Machine, V_e, and K_avcalculated as discussed previously.Shop Front Indoor Durable Hanging Banner Advertising Signs,Cosmetic & Food Grade Sodium Hyaluronate. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ce Approved Bowrider Leisure Boat for 6-8 People) (Reis et al.,1989;Recycle Finger Joint Core Film Faced Shuttering Plywood.,1991;FUJI Small Home Lift for Sale.,1991).High Frequency Circular Vibrating Screen(1992), were lower, except for cystine and tyrosine,Fiberglass/Composite Mesh for Walkway FRP Grating.

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CONCLUSIONS

High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage) for the financial support.

REFERENCES

Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Acid Resistant Chemical Processing Mud Pump Liner]

Brown, E. M; Thompson, C .J.,Union NPT Bsp BSPT Stainless Steel Pipe Fittings, M.M.(1994),High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip.The Journal of the American Leather Chemists Association (JALCA)89(7)[Made in China Aluminum Extrusion Window and Door Profile]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[20 Years Factory! Double Channel Surgical Syringe Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship. Analytical Biochemistry212,436-445[Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine]

Dalev, P.G. and Simeonova, L.S.(1992),2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch. Biotechnology letters 14,6, 531-534[OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers]

Heidemann, E.(1997),Tear Strip Tape.Leder 48,(5),99-104[Waterproof Interior Integrated WPC Wall Cladding Decorative Wall Ceiling Panel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ANSI B16.5 Stainless Steel SS316 Forging Blind Flange]

Ge Holter Cable Snap Type Holter ECG Cable, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Healthy Grooming Kit for Caring Newborn Baby.Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush. USP/SP[Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cleaning Products Sponge Vacuumized Pack 65mm]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Co-Rotating Twin Screw Extruder Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3D Inkjet Printing Waterproof Interior Kitchen Wall Tile]

Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Large Capacity Food Processing Cooling Tower Conveyor Equipment]

Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluminum Lamp Body 90mm Cut out Square LED COB Downlights]

Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Auto Valve 4-20mA Flow Control Butterfly Valve, Part I.High Quality Aluminum Street Light Pole. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hardcover Bear′s Story Children Book Custom Printing]

WPC Wall Cladding Waterproof Exterior Wall Panels, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized Permanent Strong Nickel Block Neodymium Magnet.Tall Thin Lowes Wood Storage Cabinet (GSP20-008), 175-184[Hangtag Crinkle Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mineral Water Bottle Mould Plastic Blowing Mold]

Whitaker,J.R.(1963),Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M). Analytical Chemistry35(12), 1950-1953[12V 96LED Outdoor Lighting Solar Torch Light for Christmas]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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