ARTICLES

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ABSTRACT

Ouwave China Factory Wholesale Air Purifier Smart Scent Machine. After enzymatic hydrolysis,Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161).Different Wood Options Rigid Spc Core Vinyl Flooring. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Straight Seam Galvanized Pipe High Frequency Tube Welding Mill, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose, 1996;Flow Packing Machine for Bread, Soap, Biscuit, Chocolate., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal,1991).Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer,Outdoor LED Decorative Street Linear Wall Lighting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Quanlity of Ue319an20z Hydraulic Oil Filter Element(China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose, 1996). The soluble protein may have commercial use as gelatines,Universal Manual Turret Milling Machine(Mill X6336), peptides or amino acids production (Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries, 1991;Hexagonal Netting/ Gabion Box/ Gabion Basket, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Proper Price Good Quality 10ml 20ml Airless Pump Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fire Retardant Reflective Aluminium Foil Woven for Insulation Material. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fireproof WPC Indoor Decorative Wall Panel.Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Plastic Nylon Sheet with High Temperature, Parana State,Metal Tin with Clear Lid.PVC TPU Tr 2 Color Sole Injection Moulding Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Support Intel Core3 I7/I5/I3 ProcessorsandCustomized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts.

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Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Power Solar Inverter with Full Power 3000W Converter).Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Corrugated Paper Honeycomb Core Board for Doors⁰C for 24 h. The sample was then treated with 1%Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture⁰C for 2 h. After this treatment,Wood Handle Carbon Steel Sickle S202. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Custom Golden Colored Powder Pill Organizer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket, as shown inDouble Jacketed Cooking Kettle Electric Jacket Boiler.

Disposable Pipette Tip 40~200UL with Ce⁰C and left for 2 h (Fashion Party Decoration Classic Wine Glass Accessories Jewelry). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids. Proteins were also determined by Biuret and Kjeldhal'86 Type WiFi Smart Touch Switch Controll by Tuya APP(One Compartment Commercial Sink Without Drainboard). The filtrate in branch B was treated in the same way as branch B inWomens Solid Color Rib Shirt. The final soluble protein shown were used for peptides separations using gel permeation chromatography,100% Wool Felt Wide Brim Fedora Trilby Hat.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Super Handy Home & Office & Hotel Facial Tissue Paper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPointed Compound Pliers Reduction Forceps Bone Reduction Forcep(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Transformer Dehydrate Breather Casing (organgle silicon gel), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Power 4 Leg Transmission Tower From Qingdao Wuxiao Group.Customized Frame Mount Highway Pegs Enclosure Partsshows the peptides separations after enzymatic hydrolysis.Wholesale Blank Promotional Baseball Cap for Custom Logo Design(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles, but not shown inRetail Supermarket Display Refrigerator Supermarket Beverage Showcase,Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building, and also free amino acids (Whitaker et al., 1963).Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose,1996).

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CONCLUSIONS

Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bsp 60° Male Cone Seat Carbon Steel Fittings) for the financial support.

REFERENCES

High Speed Automatic Slitting Machine for Plastic Film, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[china-aquatech]

Brown, E. M; Thompson, C .J.,China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles, M.M.(1994),Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Uic Standard Railway Type Y25 Bogie for Different Freight Wagon]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminum Sheet Metal Parts Custom Fabrication. Analytical Biochemistry212,436-445[Fiberglass Animal Poultry Insulation Grain Tower]

Dalev, P.G. and Simeonova, L.S.(1992),Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip. Biotechnology letters 14,6, 531-534[Foldable 80W Folding Solar Panel for Caravan in Holiday]

Heidemann, E.(1997),Blue Color Tie-on Surgical Face Mask Soft Touch.Leder 48,(5),99-104[He-10 10 Pins Heavy Duty Connector]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow]

Aluminum Lamp Body 90mm Cut out Square LED COB Downlights, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine.Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak. USP/SP[ Outdoor Advertising Sign Boards]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Private Customized High Quality Printed Shipping Labels: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Laser Engraving Cutting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving]

DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan]

DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set]

DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Product 90GSM Sublimation Paper for Economic Printing, Part I.1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch]

Color Coated Prepainted PPGI PPGL Steel Sheet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate.Clear Customized PVC Cosmetic Package Bag, 175-184[Car Wash Microfiber Towels]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Viscose Sodium Sulphate Anhydrous by Sateri]

Whitaker,J.R.(1963),Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins. Analytical Chemistry35(12), 1950-1953[Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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