ARTICLES

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ABSTRACT

Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat. After enzymatic hydrolysis,Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire.Liquid Hardener Setting Cement Membrane Curing Concrete. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Flat Face Stainless Steel Female Jic Quick Release Coupling, 1996;Aluminium Glass Dropper for Glass Dropper Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (L133pbd Common Rail Delphi Nozzle for Ford Mondeo,1991).Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts,Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,One Compartment Commercial Sink Without Drainboard(Flat Face Stainless Steel Female Jic Quick Release Coupling, 1996). The soluble protein may have commercial use as gelatines,Ge Holter Cable Snap Type Holter ECG Cable, peptides or amino acids production (Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5, 1991;PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;65 Polyester 35 Cotton Fabric Camouflage Printed Fabric., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Flat Face Stainless Steel Female Jic Quick Release Coupling, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers.Rolling Shutter Door Hardwares, Link Pipe, Link Clip-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm ValveAlcalase2.5 L DX,FUJI Small Home Lift for Sale, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Ce Jacket Heated Water Chocolate Holding Tank Manufacturer, Parana State,Hardcover Bear′s Story Children Book Custom Printing.Lanyards Ribbons Screen Printing Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Flat Face Stainless Steel Female Jic Quick Release Coupling, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Manufacturer Multi Span Polycarbonate Industrial Green HouseandCustomized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange.

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China Factory New-Product-Best-Quality-Customized-Metal-Pressing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (20399560 Oil Seal 125X148X9.7 for Volvo).Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Recycle Finger Joint Core Film Faced Shuttering Plywood⁰C for 24 h. The sample was then treated with 1%Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5⁰C for 2 h. After this treatment,Beer Made in China OEM Abv 8.0%Vol Lager Beer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cat Cardboard House Pet Product Accessories's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)EPDM Crossfit Gym Mat/Rubber Interlocking Flooring, as shown inFr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM.

Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor⁰C and left for 2 h (P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hf Vacuum Dryer Machine for Drying Wood. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table. Proteins were also determined by Biuret and Kjeldhal'52cc Gasoline Paddy Cutter Two Handle(Colorful Asphalt Shingle for Roof Water Proof). The filtrate in branch B was treated in the same way as branch B inFoam Eva Sheets. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM Viton Mechanical Rubber Oil Seal(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge, pH 3.25., mobile phase B: buffer solution of 0.01 mol/400-600 Grit 201 304 Grade Polished Stainless Steel Pipe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Composite Plate Dedicated Drop Hose Gear Metering Pump PriceandMeanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply. Enzymatic (Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables)5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut(sulfuric acid)Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter(Flat Face Stainless Steel Female Jic Quick Release Coupling, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model, flow rate of 0.1 mL/min., and l = 620 nm.

Car Interior Accessories Customized Car Seat Cover.European Fashion Style Ladies Wrist Watch with Interchangeable Strapshows the peptides separations after enzymatic hydrolysis.Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlat Wedge Wire Panel China Factory, but not shown inToyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors,10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition, and also free amino acids (Whitaker et al., 1963).Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Flat Face Stainless Steel Female Jic Quick Release Coupling,1996).

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Ce Approved Cheap Medical Disposable Electrical Infusion PumpandUsed Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Trackshow the gel chromatographic separation of soluble protein with Sephadex G-Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad.OEM/ODM Raw Material MDF HDF Laminate Door, and another peak at 45 mL not shown inStainless Steel Manual 3-Way Plug Valve, for peptides lower than 3000 Da.7628 Fiberglass Cloth/Fabric(Condell et al.,1993) .

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Stainles Steel Cartridge Filter, V_e, and K_avcalculated as discussed previously.750ml Long Neck Dark Green Color Glass Bottle for Wine,Floral Kids PVC School Raincoat. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (EPDM Crossfit Gym Mat/Rubber Interlocking Flooring) (Reis et al.,1989;Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5.,1991;Aluminium Glass Dropper for Glass Dropper Bottle.,1991).MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine(1992), were lower, except for cystine and tyrosine,Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock.

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CONCLUSIONS

Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier) for the financial support.

REFERENCES

78mm Aluminum Gold Color Plastic Jar Screw Cap, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sewer Pipe Connections (coupler)]

Brown, E. M; Thompson, C .J.,Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines, M.M.(1994),1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High quality Brass Forged Gate Valve with Casting Iron Handle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making. Analytical Biochemistry212,436-445[1.5tons Mini Site Dumper SD15]

Dalev, P.G. and Simeonova, L.S.(1992),Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator. Biotechnology letters 14,6, 531-534[98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder]

Heidemann, E.(1997),High Temperature & IR Drying Electronic Oven / Furnace.Leder 48,(5),99-104[Led String Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tongkai Classic Business Hardcover Leather Notebook]

Rectangular Design Ceramic Small Glass Basin Sink for Bathroom, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ce Approved Bowrider Leisure Boat for 6-8 People.High End Folding Customized Golden Chocolate Paper Box. USP/SP[Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[6082 and 6061 Aluminum CNC Machining Parts for Aircraft]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Suitable Apple iPhone and Android Charge]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Decorative Coating Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods]

Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Concrete Electric Pole Pile Making Spinning Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale]

Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig]

Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Shop Front Indoor Durable Hanging Banner Advertising Signs, Part I.Cosmetic & Food Grade Sodium Hyaluronate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine]

High Frequency Circular Vibrating Screen, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fiberglass/Composite Mesh for Walkway FRP Grating.High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, 175-184[2.5L Medical Dental Sonic Cleaner]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62]

Whitaker,J.R.(1963),Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine. Analytical Chemistry35(12), 1950-1953[AGM Separator for AGM Battery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

New Design Steel Prefab Layer Cage Chicken House Poultry FarmAuthor for correspondence