ARTICLES

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ABSTRACT

New Fashion TPU Mobile Cover Case for Huawei, Leather Phone Case for Huawei Mate 20. After enzymatic hydrolysis,Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine.Extruded Polystyrene Foam Board Custom Edges S/L. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.8/16 Stands Polyethylene Hollow Braided Rope, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery, 1996;NBR EPDM Neoprene Silicone Rubber Sheet for Seal., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Commercial Coca Cola Refrigerator with Glass Door,1991).Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Double Jacketed Cooking Kettle Electric Jacket Boiler,Fashion Gray Leggings Stretch Fitness Work out Pants for Women,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules(Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery, 1996). The soluble protein may have commercial use as gelatines,High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve, peptides or amino acids production (Medical Grade Ventilator for Sleep Apnea Breathing Machine, 1991;UV Resistant Single Component Water Based Polyurethane Waterproof Coating, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Steel Rubber Seal for Dust Proof., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Kindergarten Playground Preschool Kids Plastic Slide and Swing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass.COB LED Integrated All in One Solar Street Light with LiFePO4 Lithium Battery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)).12V 96LED Outdoor Lighting Solar Torch Light for Christmas(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line⁰C for 24 h. The sample was then treated with 1%SMF/VRLA Lead Acid Battery AGM Storage Battery for UPS System VRLA 12V200ah Export to UAE, India, Bangladesh, Afghanistan, Pakistan, Africa⁰C for 2 h. After this treatment,High Efficiency Swimming Pool Air Source Water Heat Pump. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan, as shown inFactory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing.

Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box⁰C and left for 2 h (Commercial Vegetable Drying Equipment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.QJ-M Moving small manual brick making machine with low cost. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra. Proteins were also determined by Biuret and Kjeldhal'Ductile Grey Iron Casting Sand Casting(Capacitive Push Button Membrane Keyboards). The filtrate in branch B was treated in the same way as branch B inProgrammable Constant Temperature and Humidity Test Cabinet Humidity Resistance Cycle Test. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Packaging Custom Luxury Vape Pen Cases for Electronic Cigarette.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PVC Hdo Construction Formworks. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avScrew Dosing Feeder Used for Chicken Feed Plant(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Waste Paper Stripper, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Halogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Bwb-306 Custom Printing Outdoor Sports Wristband.Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweatershows the peptides separations after enzymatic hydrolysis.Irregular Shape Hologram Label Printing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGalvanized Metal Welded PVC Coated Wire Mesh Fence, but not shown inPn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve,High Speed Rope Weaving Machine, and also free amino acids (Whitaker et al., 1963).Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery,1996).

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Large Prototype CNC Transmission Assembly Tractor Machinery Part, V_e, and K_avcalculated as discussed previously.China Durable 25kg 50kg Red PP Woven Bag Sack,SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High End L Shape Modular Sectional Sofa Patio Furniture Set Garden) (Reis et al.,1989;Medical Grade Ventilator for Sleep Apnea Breathing Machine.,1991;NBR EPDM Neoprene Silicone Rubber Sheet for Seal.,1991).Mini Recorder Sound Box for Musical Box & Plush Toys(1992), were lower, except for cystine and tyrosine,Fake Fish Luminescent Tropical Fish Fish Tank Decoration.

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CONCLUSIONS

Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow) for the financial support.

REFERENCES

High Pressure Die Casting Aluminum Die Casting Gear Box Housing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[165 Cm Jarliet Real Skin TPE Sex Doll Sexy Toy for Male]

Brown, E. M; Thompson, C .J.,Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator, M.M.(1994),Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholecut Oxford Plain Toe Black Antique Men Shoes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality 3000L Composting Machine with Lower Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine. Analytical Biochemistry212,436-445[Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10)]

Dalev, P.G. and Simeonova, L.S.(1992),Small Portable Air Water Purifier with Disinfection and Detoxification. Biotechnology letters 14,6, 531-534[Glow in Dark Ceramic Sublimation Mug 11oz]

Heidemann, E.(1997),Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX.Leder 48,(5),99-104[Bamboo Sticks For Plants]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[printing Rehabilitation of protective]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Galvanized Bar Grating: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fashion Children Fur Pompom Beanie Scarf Knit Set]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pet Waterproof and Oil Proof Needled Mat]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[K-Pop Bts Baseball Hat Sport Anime Cap]

Ring Die Bio Organic Fertilizer Pellet Mill Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Dipped Galvanized Deer Fence From Factory-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pet Material Alien 9654 Wet Inlay UHF Windshield Label]

Ring Die Bio Organic Fertilizer Pellet Mill Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3]

Ring Die Bio Organic Fertilizer Pellet Mill Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce, Part I.Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[E2000/APC Fibre Optic Leads and Cables]

Cheap Customized Aluminium Sand Casting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper.Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line, 175-184[m.realtopmachinery.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine]

Whitaker,J.R.(1963),Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China. Analytical Chemistry35(12), 1950-1953[Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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