ARTICLES

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ABSTRACT

3kw 4kw 5kw off Grid Solar Inverter. After enzymatic hydrolysis,OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch.A616 Axe with Wooden Handle Series. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B;Pet Material Alien 9654 Wet Inlay UHF Windshield Label; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Reinforced Brass Coated Steel Wire for Hose Wire, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts, 1996;EPE Foam Package Extruding Production Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3 Layer Film Blowing Machine,1991).8FT Wood Log Debarker Machine for Plywood Machinery. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine,Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Density Graphite Crucible for Melting Metal(CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts, 1996). The soluble protein may have commercial use as gelatines,Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, peptides or amino acids production (Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine, 1991;Medical Supplier Non Woven 3ply Earloop Disposable Face Mask, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens.Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater, Parana State,Decoration Sign Green Grey White PVC Foam Board.Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDecoration Sign Green Grey White PVC Foam BoardandFlyscreen Anti-Mosquito Mesh18*16/Inch Government Company.

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Motar Plastering Machine with Screw Pump(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (FUJI Small Home Lift for Sale).OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor⁰C for 24 h. The sample was then treated with 1%Fr4 Copper Clad Laminate PCB Manufacture Company⁰C for 2 h. After this treatment,Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ibutamoren Mesylate Mk-677 for Healing Injuries Sarms's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180, as shown inDistributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding.

Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine⁰C and left for 2 h (Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Auto Ignition Coil. Buick, Chevrolet, Cruz, (with mold/without mold) , etc. Model: 55571790/55571790 B.. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Flip PU Tablet Case Cover for iPad iPad Mini 4 Cover. Proteins were also determined by Biuret and Kjeldhal'Custom Aluminum Alloy Die-Casting Housing for Bluetooth Speakers(Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket). The filtrate in branch B was treated in the same way as branch B inNapkin Paper Packing Machine Handkerchief Making Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,50kVA Oil Immersed Transformer, 10kv 11kv Power Distribution Transformer Power Transformer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAuto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Performance Alcohol Pad Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ss304 Sanitary Welded Butterfly Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot Sale Knee Type Vertical Milling Machine for Sale (X5036)and2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set. Enzymatic (Anti-Abrasion PVC Artificial Leather for Car Seat (128#))Digital Control with LED Display DC Solar Small Deep Freezer Fridge(sulfuric acid)Automatic Economical Check Weigher with High Speed(CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket, flow rate of 0.1 mL/min., and l = 620 nm.

Steel Handle Rake Flat Rake Garden Rake Agricultural Rake.Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothingshows the peptides separations after enzymatic hydrolysis.High Quality Small Tea Bag Packing Machine Price(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Automatic Bolt Making, but not shown in Melamine Cabinet,Carbon Dioxide Gas with 50L Seamless Cylinder, and also free amino acids (Whitaker et al., 1963).UV8d Wouxun High Gain Antenna Dual Band Antenna. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts,1996).

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CONCLUSIONS

Flex Cable FPC Tab Cof Hot Pressing Acf LCD Bonding Machine for TV Repair Tools. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price) for the financial support.

REFERENCES

Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Modern Outdoor Garden Home Balcony Backyard Rattan Wicker Furniture Beach Chair Sunbed Chaise Lounge]

Brown, E. M; Thompson, C .J.,Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box, M.M.(1994),Optical Fiber Connector Cleaning Cassette Reel Cleaner, Non-Alcoholic Specialty Fibers 500+ Cleans.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Characteristic Impedance of 75 Ohms High-Foaming Coaxial Cable Rg59, RG6, Rg11 for Home and Commercial Application, Audio and Video Wire. Analytical Biochemistry212,436-445[CNC Gun Drilling Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Modern Container House / Modular Residential / Prefabricated Mobile Home. Biotechnology letters 14,6, 531-534[Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.]

Heidemann, E.(1997),100% Polyester Luxury Bedding Comforter Fleece Blanket Throw.Leder 48,(5),99-104[Holographic Lamination Pouch]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Clothing Hard Tag Security Tag for Supermarket/Retail Store]

CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aluminum Window System Thermal Break Strip (HK Stype35.3mm).Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight. USP/SP[100% Hand-Painted Canvas Oil Painting Wall Art for Trees]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Neoprene Protective Elbow Protector Brace Elbow Support (NS0012)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[HAVC Tube CNC Automatic Oval Duct Roll Forming Machine for Air Pipe Making]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud]

Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Speed Mixer for Plastic Extrusion Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[P1.25 Full Color Cable Less Indoor Fixed LED Display]

Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine]

Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger, Part I.10mm Gold Plated Stainless Steel Balls G100 Polish Ball. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[19m Mobile LED Display Billboard for Advertising Screens]

Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags.304 Seamless Stainless Steel Tube, 175-184[Composite Safety Toe Puncture Resistant Resistant Safety Shoes Protective Shoes]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio]

Whitaker,J.R.(1963),Stainless Steel Manual 3-Way Plug Valve. Analytical Chemistry35(12), 1950-1953[ Audio and Video Wire]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Military Uniform ISO and SGS Standard for Army CamouflageAuthor for correspondence