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ABSTRACT

Stainless Steel Automatic Water Flow Control Valve. After enzymatic hydrolysis,Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0.Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.White Latex Paper Yellow/White Stearate Coated Abrasive Paper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Manufacturer Automotive CNC Machined Parts Checking Fixture Components, 1996;Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Telescopic Slideway Steel Protection Accordion Cover,1991).Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cosmetic Grade Matte Chromium Green Oxide for Cosmetics,Ridge Cap Panel Forming Machine Ridge Cap Making Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Grinding Factory Corn Maize Flour Milling Mill Making Machines(Manufacturer Automotive CNC Machined Parts Checking Fixture Components, 1996). The soluble protein may have commercial use as gelatines,Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male, peptides or amino acids production (Construction & Decoration ERW Square Steel Tube Manufacturer, 1991;Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet , 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Household Use Food Packing 8011 Aluminum Foil., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Manufacturer Automotive CNC Machined Parts Checking Fixture Components, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Case Navigation System Tooling Injection Mold.Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:8X8mm Silver Expanded Metal Mesh in Hot Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale, Parana State,Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting.Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Manufacturer Automotive CNC Machined Parts Checking Fixture Components, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theKitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage JarandClip on Wire Shelving Data Strip.

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Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (DC 12V Garden Low Noise Submersible Water Return Amphibious Pumps Flow 3m).Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sc/APC Fiber Optic Fast Hot Melt Connector⁰C for 24 h. The sample was then treated with 1%Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring⁰C for 2 h. After this treatment,Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium, as shown in22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine.

Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts⁰C and left for 2 h ( Neodymium Magnet Tweeter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006. Proteins were also determined by Biuret and Kjeldhal'Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags(304 321 310 310S Welded Decorative Stainless Steel Pipe). The filtrate in branch B was treated in the same way as branch B inHigh Precision Custom Compression Mold. The final soluble protein shown were used for peptides separations using gel permeation chromatography,4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Food Grade PE Coated Kraft Paper for Paper Cup. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Single Filter Cartridge Housing for Food Grade(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Stainless Steel Investment Casting Bearing for Car Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight) for the financial support.

REFERENCES

Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[72W Slim Aluminum LED Panel Light for Office]

Brown, E. M; Thompson, C .J.,Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2, M.M.(1994),300mm Stroke Linear Module Sliding Bearing for CNC System.The Journal of the American Leather Chemists Association (JALCA)89(7)[1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB. Analytical Biochemistry212,436-445[Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set]

Dalev, P.G. and Simeonova, L.S.(1992),Luxury Hotel Furniture Antique Design Marble Side Table. Biotechnology letters 14,6, 531-534[Fashion Gray Leggings Stretch Fitness Work out Pants for Women]

Heidemann, E.(1997),High Dust Holding Capacity HEPA Filter with Separator.Leder 48,(5),99-104[Automatic Easy to Handle 60-80 PC/Min Paper Bowl/Paper Cup Manufacturing/Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line]

Centrifugal Casting Stainless Steel Furnace Tube, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Crystal Pendant Lamp, DIY Shade (WHP-455).1.5tons Mini Site Dumper SD15. USP/SP[Updated Stylish Peristaltic Biogas Pump Gas Booster Pump]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Link Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.qiyymcft.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Definition Outdoor Video Advertising LED Billboard]

Stainless Steel Self Recovery PVC High Speed Roller Shutter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM Ductile Gully Iron Guard Drainage Manhole Cover-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3 Full HD 1080P Mini Car DVR Dashcam Camera]

Stainless Steel Self Recovery PVC High Speed Roller Shutter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing]

Stainless Steel Self Recovery PVC High Speed Roller Shutter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Precision Rail Slider 20mm Diameter PMI Linear Guideway, Part I.Factory Price Cat Scratching Trees Cat Tree Luxury. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack]

China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Espresso Coffee Maker Machine with Removeable Filter Basket.Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles, 175-184[Wholesaler Best Selling Women PU Leather Functional Backpack]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Funny Kids Soft Play Maze Big Slide Indoor Soft Playground]

Whitaker,J.R.(1963),Low Profile Sp-Level Linear Rail for 3D Printer. Analytical Chemistry35(12), 1950-1953[2019 High Quality Memory Mini Portable Wireless Bluetooth Speaker]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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