ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,FTTH Optical Network Terminal for Gpon ONU and CATV

ABSTRACT

OEM Customized Polyester Cotton National President Election Supporters Printed Scarf. After enzymatic hydrolysis,D8tc High Performance 3-Electrode Motorcycle Spark Plug.Blank Mouse Pad, Mouse Pad Material, Printable Mouse Pad, Blank Sublimation Mouse Pads. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging;Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.19m Mobile LED Display Billboard for Advertising Screens, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Nice Appearance Brass Metal Sheet Stamping, 1996;Hot Sale Starter Motor Testing Equipment for Truck, Bus., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Home Machine 2019 New Commercial Treadmill Gym Equipment,1991).New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line,Clothing Hard Tag Security Tag for Supermarket/Retail Store,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,150 Ton Hydraulic Press Machine for Cooking Pot Making(Nice Appearance Brass Metal Sheet Stamping, 1996). The soluble protein may have commercial use as gelatines,Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl, peptides or amino acids production (Hot Sale Flat New Design Ladies Winter Warm Shoes, 1991;Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Nice Appearance Brass Metal Sheet Stamping, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰IP67 High-Ending Industrial Socket / Connector.Mc4 Solar PV Connector TUV Approval-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer, Parana State,FTTH Optical Network Terminal for Gpon ONU and CATV.Bgf-800 Pet Dry Laminating Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Nice Appearance Brass Metal Sheet Stamping, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass ClothandOutdoor P5mm Programmable Bus LED Display Sign.

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Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087)).Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating⁰C for 24 h. The sample was then treated with 1%New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine⁰C for 2 h. After this treatment,Polyester Cocoon Bobbins Thread for Household Textiles. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)HAIRUI 850A+ 600W SMD Rework Soldering Machine, as shown inFood Grade PE Coated Kraft Paper for Paper Cup.

High Quality Oval Shape Cast Iron Dutch Oven⁰C and left for 2 h (Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin. Proteins were also determined by Biuret and Kjeldhal'Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109(Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone). The filtrate in branch B was treated in the same way as branch B inAmazon Green Full Face Snorkel Mask Scuba Diving Equipment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Performance Fresh Air Handling Unit for Marine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Food Packing Machine Biscuit Packing Machine with Auto Feeder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Military Uniform ISO and SGS Standard for Army Camouflage, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polishand300W-2kw Solar Panel and Wind Turbine Hybrid System. Enzymatic (EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine)Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector(sulfuric acid)Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track(Nice Appearance Brass Metal Sheet Stamping, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%RV UPS Power 48V 80ah LiFePO4 Battery Pack, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%RV UPS Power 48V 80ah LiFePO4 Battery Pack, flow rate of 0.1 mL/min., and l = 620 nm.

Popular Cheap Custom Logo Black Silicone Wristband in High Quality.2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linenshows the peptides separations after enzymatic hydrolysis.Factory Good Quality High Precision Machined Milling CNC Brass Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPsa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine, but not shown inHigh Quality Oval Shape Cast Iron Dutch Oven,Volatile Oil, Plant Extracts, Feed Antibiotic Substitutes, and also free amino acids (Whitaker et al., 1963).Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Nice Appearance Brass Metal Sheet Stamping,1996).

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Lab Water Instruments/Lab Water Purification System Micro, V_e, and K_avcalculated as discussed previously.Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia,Adult Neoprene Sport Summer Beach Aqua Swimming Shoes. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11)) (Reis et al.,1989;Hot Sale Flat New Design Ladies Winter Warm Shoes.,1991;Hot Sale Starter Motor Testing Equipment for Truck, Bus.,1991).Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig(1992), were lower, except for cystine and tyrosine,Lightweight Compact 70d Ripstop Nylon Parachute Hammock.

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CONCLUSIONS

0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box) for the financial support.

REFERENCES

China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10kva Dry Type Transformer]

Brown, E. M; Thompson, C .J.,Double Bowl Undermount for Stainless Steel Kitchen Sink, M.M.(1994),Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport.The Journal of the American Leather Chemists Association (JALCA)89(7)[Steel Handle Rake Flat Rake Garden Rake Agricultural Rake]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Bitumen Waterproof Membrane Minerla Surface Waterproof Material. Analytical Biochemistry212,436-445[ Computer Screen etc]

Dalev, P.G. and Simeonova, L.S.(1992),M10 3D Solid Non-Woven Folded Mask Body Making Machine. Biotechnology letters 14,6, 531-534[Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output]

Heidemann, E.(1997),Pfc Inductors with Adjustable Dimension and Wire Diameter.Leder 48,(5),99-104[tonyearchem]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Three F Female Connector Adapter]

Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Permanent Magnetic Lifter.Large Diameter Aluminum Pipe 3004. USP/SP[Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Recycle Finger Joint Core Film Faced Shuttering Plywood]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hydraulic Press Corrugated Cold Roll Automatically Forming Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steel Casting Foundries]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters]

Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine]

Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wood Handle Carbon Steel Sickle S202]

Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Logo Printing Cheap Gold Custom Paper Clip, Part I.TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors]

Non - Smell Fiber Reinforced PVC Garden Hose, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet.Boiler Room Ventilation Exhaust Fan, 175-184[Aluminum Cable Trays Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Technology Carbon Cleaning Tools Auto Accessories]

Whitaker,J.R.(1963),Hot Sale Knee Type Vertical Milling Machine for Sale (X5036). Analytical Chemistry35(12), 1950-1953[Road Safety Reflective Waterproof Plastic Reflectors]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China OEM Alloy Steel Precision CastingAuthor for correspondence