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ABSTRACT

3D Creative Design Metal Medal Promotion Gift of Sport. After enzymatic hydrolysis,HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line.Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish, 1996;Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts,1991).Puffed Core Filling Sanck Production Machine with Ce From China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting,Cummins Fuel Injector Kit (3076736 3095023),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5(Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish, 1996). The soluble protein may have commercial use as gelatines,Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch, peptides or amino acids production (Plastic Therm Forming Filling Sealing Machine, Lid Press Machine, 1991;Electric 100W Three Wheels Toy Car for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Design Customized 100% Cotton Home Towel Bath Towel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Toughened Railing Glass.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Delrin Sheets with Good Wear Resistance. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery.Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Tc Made Boy′s Short Pants).Non Woven Fabric for Shoe Lining Shoe Inner Good Quality(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking⁰C for 24 h. The sample was then treated with 1%HDPE Large Diameter Hollowness Wall Winding Pipe Production Line⁰C for 2 h. After this treatment,Non-Woven Medical Outside Earloop Mask Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Professional Family Nonstick Cast Iron Frying Pan's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric, as shown inCustom 2 Ring Binder PP Lever Arch Clip File Folder.

Fashionable Clothing Garment Paper Swing Tags (ST-01)⁰C and left for 2 h (Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Horizontal Directional Drilling Machine SHD28. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electrial Galvanized Conduit Box/Gi Pipe Fitting. Proteins were also determined by Biuret and Kjeldhal'Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar(QJ-M Moving small manual brick making machine with low cost). The filtrate in branch B was treated in the same way as branch B in93% Oxygen Generator. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High quality Brass Forged Gate Valve with Casting Iron Handle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.TV Stand Carving European LED Lights Heating Fireplace (320SS)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Mini LED Flood Light SMD Flood Light 150W, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Low Price Fashion Unique Plastic Zipper, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Electric DC 24V Geared Motor for Auto Doors. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof) for the financial support.

REFERENCES

Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Microcurrent LED Skin Rejuvenation Phototherapy Ultrasound Face Lift Beauty Machine Home Use]

Brown, E. M; Thompson, C .J.,RF Shielding MRI Door with Brass Frame for RF Cage, M.M.(1994),Geotextile Beg Used for Beach Restoration Geobag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reusable Silicone Food Storage Bag. Analytical Biochemistry212,436-445[Medical Ce Approved Bp Machine Blood Pressure Monitor]

Dalev, P.G. and Simeonova, L.S.(1992),Dental Emax Empress Crown From China Dental Lab. Biotechnology letters 14,6, 531-534[Professional Pulse Dust Filter for Grain Processing]

Heidemann, E.(1997),Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt.Leder 48,(5),99-104[Hydraulic Cylinder Pu Wiper Seals]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[50kVA Oil Immersed Transformer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hexagonal Netting/ Gabion Box/ Gabion Basket]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dubai Products Rectified Tile for Wall: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customization! Bdy380CD Horizontal Directional Drilling Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Smash-Proof Puncture-Proof Safety Shoes Labor Shoes Safety Footwear]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer]

ANSI B16.5 Stainless Steel SS316 Forging Blind Flange, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC]

ANSI B16.5 Stainless Steel SS316 Forging Blind Flange,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Popular Cheap Custom Logo Black Silicone Wristband in High Quality]

ANSI B16.5 Stainless Steel SS316 Forging Blind Flange, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer, Part I.Integrated Hydraulic Steering Control Units (BZZ1F SCU). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment]

New Model Water Source Heat Pump (SLW100D), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment.Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube, 175-184[409]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic Gas and Water End Protective Covers (YZF-H26)]

Whitaker,J.R.(1963),10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets. Analytical Chemistry35(12), 1950-1953[Best Quality for Stretch Film Jumbo Roll]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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