ARTICLES

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ABSTRACT

Corrugated Paper Honeycomb Core Board for Doors. After enzymatic hydrolysis,Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture.Wood Handle Carbon Steel Sickle S202. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Disposable Pipette Tip 40~200UL with Ce, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale, 1996;1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (86 Type WiFi Smart Touch Switch Controll by Tuya APP,1991).100% Wool Felt Wide Brim Fedora Trilby Hat. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Super Handy Home & Office & Hotel Facial Tissue Paper,Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hidden Handle Safe for Children in Bathroom Cabinet(Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale, 1996). The soluble protein may have commercial use as gelatines,Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale, peptides or amino acids production (Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets, 1991;Transformer Dehydrate Breather Casing (organgle silicon gel), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3 Full HD 1080P Mini Car DVR Dashcam Camera., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality X Ray Lead Glasses with Good Prices. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Galvanized Steel Track for Sectional Garage Door.Fashion 925 Sterling Silver Pendants Necklace Jewelry-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Power 4 Leg Transmission Tower From Qingdao Wuxiao Group(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives).Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Pulse Dust Filter for Grain Processing⁰C for 24 h. The sample was then treated with 1%Plug-in Assembly Type Straight Through Joint and Insulation Joint⁰C for 2 h. After this treatment,DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Speed Automatic Slitting Machine for Plastic Film, as shown inHigh Pressure Die Casting Aluminum Die Casting Gear Box Housing.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIlife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Product 90GSM Sublimation Paper for Economic Printing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Shoelace-Free Climbing Shoes with Wire Button. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine) for the financial support.

REFERENCES

Kraft Paper Laminated Aluminum Foil, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Flow Dividing Valve]

Brown, E. M; Thompson, C .J.,China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800, M.M.(1994),Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600.The Journal of the American Leather Chemists Association (JALCA)89(7)[500kg Capacity 14m Height Semi Electric Scissor Lift]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Watch. Analytical Biochemistry212,436-445[High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts. Biotechnology letters 14,6, 531-534[White UTP Cat5e UK Type Tooless RJ45 Keystone Jack]

Heidemann, E.(1997),150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine.Leder 48,(5),99-104[OEM Design Cheap Custom Promotional Blank Metal Aluminum Reusable Name Tag Badge for School Uniform]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Double Bowl Undermount for Stainless Steel Kitchen Sink]

PVC TPU Tr 2 Color Sole Injection Moulding Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine.CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal. USP/SP[High Speed Automatic Slitting Machine for Plastic Film]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ce Approved Bowrider Leisure Boat for 6-8 People]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Women Luxury Handbags Leather Shoulder Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Top Quality Sarms Yk11 Yk-11 Yk 11 Powder with CAS 1370003-76-1]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Barrier Terminal Blocks Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[RV UPS Power 48V 80ah LiFePO4 Battery Pack]

P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Made in China Aluminum Extrusion Window and Door Profile]

P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[20 Years Factory! Double Channel Surgical Syringe Pump]

P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Long Stroke Hydraulic Cylinder for Constraction Machine, Part I.Neoprene Protective Elbow Protector Brace Elbow Support (NS0012). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine]

Big Smart Plant Hydroponic Planter with Indoor Garden Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric.High Quality Anti-Corrosion Fiberglass Sheet and Roll, 175-184[Body Massage Shower]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers]

Whitaker,J.R.(1963),Durable Steel Retainer Plastic Road Safety Trench Cover. Analytical Chemistry35(12), 1950-1953[ANSI B16.5 Stainless Steel SS316 Forging Blind Flange]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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