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ABSTRACT

White Tight Long Sleeves off Shoulders Celebration Bandage Dress. After enzymatic hydrolysis,OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors.4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Gas Spring Strut Gas Shock for Wall Bed, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industrial Bedsheet Folding Machine (ZD-3000), 1996;Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM ODM Aluminum Casting Parts,1991).High Pressure Low Volume 2900rpm electric Water Pump. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace,Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch(Industrial Bedsheet Folding Machine (ZD-3000), 1996). The soluble protein may have commercial use as gelatines,Multifunction Copper Busbar Processing for Aluminum Material, peptides or amino acids production (Men Genuine Leather Sling Shoulder Satchel Messenger Bag, 1991;Electric DC 24V Geared Motor for Auto Doors, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.RF Shielding MRI Door with Brass Frame for RF Cage.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Geotextile Beg Used for Beach Restoration Geobag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industrial Bedsheet Folding Machine (ZD-3000), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Reusable Silicone Food Storage Bag.Dental Emax Empress Crown From China Dental Lab-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Integrated Hydraulic Steering Control Units (BZZ1F SCU)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 Male & Female Stainless Steel Quick Coupling).New Model Water Source Heat Pump (SLW100D)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment⁰C for 24 h. The sample was then treated with 1%Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube⁰C for 2 h. After this treatment,10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Capacity Vibrating Screen Manufacturer, as shown inGood Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine.

Electrochemical Polishing Tungsten Evaporation Materials⁰C and left for 2 h (Coil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,White UTP Cat5e UK Type Tooless RJ45 Keystone Jack. Proteins were also determined by Biuret and Kjeldhal'High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill(High Quality Free POM Custom Knit Acrylic Beanie Caps). The filtrate in branch B was treated in the same way as branch B inLuo Han Guo Extract/Mogroside. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cheese Puffed Snacks Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Hotfix Rhinestone Patterns Motif Logo for Decoration(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Price Spc Lvt PVC Vinyl Flooring for Home Office, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Red Papillon (JSY-3) Satin Ribbon Flower, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CNC Maching Parts for AutosandZw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch. Enzymatic (Steel Structure Construction Building Metal House)1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting(sulfuric acid)Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera(Industrial Bedsheet Folding Machine (ZD-3000), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel Sanitary Thread Pneumatic Butterfly Valve, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel Sanitary Thread Pneumatic Butterfly Valve, flow rate of 0.1 mL/min., and l = 620 nm.

2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415).Factory Truck/Bus Spray Paint Booth /Industry Paint Boothshows the peptides separations after enzymatic hydrolysis.New Design Slotted Board MDF for Wall Panel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack, but not shown inHigh Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide,Factory Wholesale Reach Standard Home and Office Furniture Fitting, and also free amino acids (Whitaker et al., 1963).Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Industrial Bedsheet Folding Machine (ZD-3000),1996).

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Oxalic Acid for 99.6% Purity

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CONCLUSIONS

Decoration Sign Green Grey White PVC Foam Board. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dubai Products Rectified Tile for Wall) for the financial support.

REFERENCES

3kw 4kw 5kw off Grid Solar Inverter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Graphite Rod Chernobyl]

Brown, E. M; Thompson, C .J.,OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch, M.M.(1994),A616 Axe with Wooden Handle Series.The Journal of the American Leather Chemists Association (JALCA)89(7)[Duct Evaporative Air Cooler for Warehouse Factory]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B. Analytical Biochemistry212,436-445[Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Capacity Vibrating Screen Manufacturer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3 Layer Film Blowing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[99% Purity Sarms Lgd-4033 Ligandrol Lgd4033 Powder for Building 1165910-22-4]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality RV Electric Seating Recliner]

8FT Wood Log Debarker Machine for Plywood Machinery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Reinforced Brass Coated Steel Wire for Hose Wire]

8FT Wood Log Debarker Machine for Plywood Machinery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PE Pipe in SDR 11 Pressing Rate for Fire-Control]

8FT Wood Log Debarker Machine for Plywood Machinery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine, Part I.High Density Graphite Crucible for Melting Metal. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mini Electric Chain Saw Cutting Machine Price]

Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine.Medical Supplier Non Woven 3ply Earloop Disposable Face Mask, 175-184[Super Market/Mall Auto Walk/Moving Walk with En81/Ce/Cu-Tr]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode]

Whitaker,J.R.(1963),UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould. Analytical Chemistry35(12), 1950-1953[Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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