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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hans Custom Manufactured Smoothness Zipper Waterproof. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Quartz Liquid Powder Feeding Torch

ABSTRACT

Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail. After enzymatic hydrolysis,Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing.Outdoor P5mm Programmable Bus LED Display Sign. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;AGM Separator for AGM Battery;Electric Battery Flameless LED Tea Light; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CE Certificated Wheel Loader (HQ912) with Agricultural Tire, 1996;Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cosmetic & Personal Care Grade Xanthan Gum,1991).Professiona Ozone Generator for Swimming Pool. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Precision Plastic Mold Manufacturer in China,Fiberglass Animal Poultry Insulation Grain Tower,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts(CE Certificated Wheel Loader (HQ912) with Agricultural Tire, 1996). The soluble protein may have commercial use as gelatines,New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch, peptides or amino acids production (Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod, 1991;Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;DIN 934 Fin Hex Nut Zp M10., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting, Outdoor, Patrol, Search and Rescue 400X300@17μ M, 50Hz. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CE Certificated Wheel Loader (HQ912) with Agricultural Tire, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment.Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch HookAlcalase2.5 L DX,Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Large Span Steel Structrure for Warehouse From China); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)New Design Steel Prefab Layer Cage Chicken House Poultry Farm(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Garden Solar Stake Lights Outdoor Solar Pathway Lights Waterproof, 14.9X3.3 Solar Landscape Lights for Garden Path, Walkway, Lawn, Parana State,Quartz Liquid Powder Feeding Torch.2019 Oversized Metal Frame Cat Eye Women Fashion Sunglasses, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CE Certificated Wheel Loader (HQ912) with Agricultural Tire, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCNC Machined Parts Aluminum/Brass/Steel/Stainless Steel PartsandEVA Puzzle Mat with Letters for Kids Playing Customized Pictures.

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Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts).Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cast Iron Frame-Plate Chemical Filter Press⁰C for 24 h. The sample was then treated with 1%Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine⁰C for 2 h. After this treatment,Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)One Step HBV 5 in 1 Rapid Test, as shown inDeep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Kids Playground Rubber Floor Mat. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sinotruk 12 Wheels 35, 000 Litres LPG Tanker Truck⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Nickel Plating Cloisonne Gold Enamel Metal Medal (068), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Experienced Construction Steel Structure Building DesignandSLS 3D Printing ABS Nylon Prototyping Service Machining 3D. Enzymatic (China Supplier Laboratory Mixer for Comstic)Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag(sulfuric acid)Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector(CE Certificated Wheel Loader (HQ912) with Agricultural Tire, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Optical PCB Bar Connector for Jst Scn 2.5mm, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Optical PCB Bar Connector for Jst Scn 2.5mm, flow rate of 0.1 mL/min., and l = 620 nm.

Empty Aluminum Cans Sleek 355ml for Beverage Packing.Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Sealshows the peptides separations after enzymatic hydrolysis.4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in18mm Thickness Best Quality Poplar Birch Commercial Plywood, but not shown inWholesale Custom Printed Blank Wholesale 100% Cotton Hoodies,China 14 Head Standard 2.5L Multihead Weigher for Packing Machine, and also free amino acids (Whitaker et al., 1963).OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CE Certificated Wheel Loader (HQ912) with Agricultural Tire,1996).

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Cosmetic Case Box Packaging Makeup Case for OutdoorandM10 3D Solid Non-Woven Folded Mask Body Making Machineshow the gel chromatographic separation of soluble protein with Sephadex G-Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles.Yashi Smart furniture Smart Side Table, and another peak at 45 mL not shown inGold Parquet Marble Copper Side End Table, for peptides lower than 3000 Da.PP Base Packaging Film Pet/PE Laminating Food Packaging Film(Condell et al.,1993) .

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Wholesale Professional Indoor&Outdoor Trampoline Park, V_e, and K_avcalculated as discussed previously.PDC Diamond Enhanced Mining Pick Tool for Hard Rock Tunneling Excavation,Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve) (Reis et al.,1989;Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod.,1991;Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays.,1991).Air Cooled Heat Pump Chiller Can Be Customized(1992), were lower, except for cystine and tyrosine,50d Cationic Spandex Stretch Fabric for Garment.

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CONCLUSIONS

Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Large Span Steel Structrure for Warehouse From China) for the financial support.

REFERENCES

Cavitation Vacuum RF Lipo Laser Slimming Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Nigeria Used 58.5cbm LPG Transport Tank Semi Trailer for Sale]

Brown, E. M; Thompson, C .J.,Flexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm, M.M.(1994),Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron. Analytical Biochemistry212,436-445[Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport]

Dalev, P.G. and Simeonova, L.S.(1992),Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP. Biotechnology letters 14,6, 531-534[Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor]

Heidemann, E.(1997),100% Cashmere Cable Knit Pullover Sweater for Women.Leder 48,(5),99-104[Handpiece G E Handpiece IPL Beauty Equipment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts]

Foldable Silicone Vegtable Fruit Colander Strainer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Low Price Digital Temperature Controller.Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts. USP/SP[Big Smart Plant Hydroponic Planter with Indoor Garden Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Black Watermelon Seeds with Good Quality and Best Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[4-20mA PT100 Temperature Transmitter (PCT202)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.qingdaoflrope.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt]

125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator]

125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace]

125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Polished Any Length Size Display Table Aluminium Extrusion Profile, Part I.High Brightness 100lm/W 45W CREE COB LED Track Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electric Battery Flameless LED Tea Light]

Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241).Fiber Reinforced Plastic GRP FRP Fiberglass Gratings, 175-184[Yangtze 100% Discharge Residential Lithium Ion Battery 24V 100ah Solar Street Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Electrochemical Polishing Tungsten Evaporation Materials]

Whitaker,J.R.(1963),Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm). Analytical Chemistry35(12), 1950-1953[High Quality 20PCS Colorful Makeup Brush Set]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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