ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Luxury Hotel Furniture Antique Design Marble Side Table. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Dust Holding Capacity HEPA Filter with Separator

ABSTRACT

Crystal Pendant Lamp, DIY Shade (WHP-455). After enzymatic hydrolysis,1.5tons Mini Site Dumper SD15.1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Precision Rail Slider 20mm Diameter PMI Linear Guideway, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Price Cat Scratching Trees Cat Tree Luxury, 1996;China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Espresso Coffee Maker Machine with Removeable Filter Basket,1991).Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Low Profile Sp-Level Linear Rail for 3D Printer,China Xr150L/Pulsar 180 Engine Parts Cylinder Kit,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Paulownia Wood Board Wood Drying Kilns for Sale(Factory Price Cat Scratching Trees Cat Tree Luxury, 1996). The soluble protein may have commercial use as gelatines,Seamless Boxer Brief for Men, peptides or amino acids production (Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning, 1991;Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lime Dewatering Filter Press Cloth Filter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-150ml Round Aromatherapy Diffuser Glass Bottle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Price Cat Scratching Trees Cat Tree Luxury, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine.100ml Square Glass Diffuser Bottle with Cap-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights, Parana State,High Dust Holding Capacity HEPA Filter with Separator.Good Quality OEM Stainless Steel Casting Rings, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Factory Price Cat Scratching Trees Cat Tree Luxury, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHousehold Use Food Packing 8011 Aluminum Foiland10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition.

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Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dubai Products Rectified Tile for Wall).Customizable Logo Plastic Safety Walking Reflectors Chinese Factory(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle⁰C for 24 h. The sample was then treated with 1%Flame Retardant FRP/GRP Round Pipe⁰C for 2 h. After this treatment,Psa Oxygen Generator for Industry (ISO9001, CE). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display, as shown in400W Emergency Backup Battery Powerstation Portable Solar Generator.

Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting⁰C and left for 2 h (Car Interior Accessories Customized Car Seat Cover). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom small plastic printed box for keychain (PET box). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fence Mesh Welding Machine. Proteins were also determined by Biuret and Kjeldhal'20 Years Factory! Double Channel Surgical Syringe Pump(2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men). The filtrate in branch B was treated in the same way as branch B inGlass Sandblasting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Quality for Stretch Film Jumbo Roll.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Drum Type Magnetic Separator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avK-Pop Bts Baseball Hat Sport Anime Cap(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Home Daily Tealight Glass Holder, Glass Candle Holder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Round Manufactory Non-Stick Silicone Stainless Dog Feeder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Lumen Outdoor IP65 100W LED Flood Light.Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermeshshows the peptides separations after enzymatic hydrolysis.Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask, but not shown in Luxury Rainfalls Shower Set,Retail Supermarket Display Refrigerator Supermarket Beverage Showcase, and also free amino acids (Whitaker et al., 1963).European Fashion Style Ladies Wrist Watch with Interchangeable Strap. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Price Cat Scratching Trees Cat Tree Luxury,1996).

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CONCLUSIONS

Best Selling Hydraulic Small Truck with Crane 10 Ton. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS) for the financial support.

REFERENCES

Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[7.0 Inch Digital Wireless Video Baby Monitor with 4 Cameras]

Brown, E. M; Thompson, C .J.,CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery, M.M.(1994),New Design Disposable Trocars for Laparoscopic Surgery.The Journal of the American Leather Chemists Association (JALCA)89(7)[CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Chocolate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Disposable Medical Combined Bacterial Viral Hme Filter. Analytical Biochemistry212,436-445[Anodized Perforated 3D Facade Windows Screen for Commercial Building]

Dalev, P.G. and Simeonova, L.S.(1992),Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale. Biotechnology letters 14,6, 531-534[Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820)]

Heidemann, E.(1997),Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System.Leder 48,(5),99-104[8 - Sodium Glycinate 99%Min]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 New Nylon Material Rapid Prototype Manufacturer in China]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Embossed Pattern PU Leather Classic Mens Wallet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Auto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Laboratory Homogenizing Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose]

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Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625]

Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Supra Brand Laundry Liquid Baby Formula, Part I.Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair]

Cleaning Products Sponge Vacuumized Pack 65mm, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mineral Water Bottle Mould Plastic Blowing Mold.High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards, 175-184[Screw Thread Cutting Tools T Slot Cutter D40-XP32 Milling Cutter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030)]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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