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FeaturedEdivaldo E.Garcia^I; Cláudia C.M.Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-One Compartment Commercial Sink Without Drainboard. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Ge Holter Cable Snap Type Holter ECG Cable

ABSTRACT

Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5. After enzymatic hydrolysis,PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass.65 Polyester 35 Cotton Fabric Camouflage Printed Fabric. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine;Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rolling Shutter Door Hardwares, Link Pipe, Link Clip, 1996;200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve,1991).FUJI Small Home Lift for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier,Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ce Jacket Heated Water Chocolate Holding Tank Manufacturer(Rolling Shutter Door Hardwares, Link Pipe, Link Clip, 1996). The soluble protein may have commercial use as gelatines,Lanyards Ribbons Screen Printing Machine, peptides or amino acids production (China Factory New-Product-Best-Quality-Customized-Metal-Pressing, 1991;Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Recycle Finger Joint Core Film Faced Shuttering Plywood., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Beer Made in China OEM Abv 8.0%Vol Lager Beer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rolling Shutter Door Hardwares, Link Pipe, Link Clip, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cat Cardboard House Pet Product Accessories.EPDM Crossfit Gym Mat/Rubber Interlocking Flooring-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fiber Reinforced Plastic GRP FRP Fiberglass Gratings).Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge⁰C for 24 h. The sample was then treated with 1%400-600 Grit 201 304 Grade Polished Stainless Steel Pipe⁰C for 2 h. After this treatment,Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut, as shown in40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer.

Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter⁰C and left for 2 h (SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Car Interior Accessories Customized Car Seat Cover. Proteins were also determined by Biuret and Kjeldhal'Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design(20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant). The filtrate in branch B was treated in the same way as branch B inChina Efficiency Waste Paper Egg Tray Making Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.7628 Fiberglass Cloth/Fabric⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainles Steel Cartridge Filter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/750ml Long Neck Dark Green Color Glass Bottle for Wine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Airtac Tubing Quick Connect Puick Coupling Pneumatic FittingandFloral Kids PVC School Raincoat. Enzymatic (MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine)Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock(sulfuric acid)Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh(Rolling Shutter Door Hardwares, Link Pipe, Link Clip, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%78mm Aluminum Gold Color Plastic Jar Screw Cap, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%78mm Aluminum Gold Color Plastic Jar Screw Cap, flow rate of 0.1 mL/min., and l = 620 nm.

Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines.Horizontal Milling and Boring CNC Lathe Machineshows the peptides separations after enzymatic hydrolysis.1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCog Mono Round Display LCD Module, but not shown inWholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric,0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making, and also free amino acids (Whitaker et al., 1963).Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Rolling Shutter Door Hardwares, Link Pipe, Link Clip,1996).

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CONCLUSIONS

High Frequency Circular Vibrating Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (52cc Gasoline Paddy Cutter Two Handle) for the financial support.

REFERENCES

Fiberglass/Composite Mesh for Walkway FRP Grating, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[300t Hydraulic Press Brake Wc67y-300t/3200]

Brown, E. M; Thompson, C .J.,High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, M.M.(1994),Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Digital Floating Baby Care Products Frog Bath Design Thermometer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Union NPT Bsp BSPT Stainless Steel Pipe Fittings. Analytical Biochemistry212,436-445[Pig Farm Cast Iron Pig Leakage Dung Floor]

Dalev, P.G. and Simeonova, L.S.(1992),High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip. Biotechnology letters 14,6, 531-534[Boiler Room Ventilation Exhaust Fan]

Heidemann, E.(1997),Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship.Leder 48,(5),99-104[Hand Push Hot Melt Road Marking Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China 14 Head Standard 2.5L Multihead Weigher for Packing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality X Ray Lead Glasses with Good Prices]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Healthy Grooming Kit for Caring Newborn Baby: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carboxymethylcellulose Sodium Salt Formula]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine]

Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp]

Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[8FT Wood Log Debarker Machine for Plywood Machinery]

Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom, Part I.Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[30t/H RO Reverse Osmosis System Water Treatment Plant]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector]

Whitaker,J.R.(1963),Customized Permanent Strong Nickel Block Neodymium Magnet. Analytical Chemistry35(12), 1950-1953[HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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