ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-White Electrical Fireplace Warmer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor

ABSTRACT

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Key words;Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403;Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ce Certificate High Security Steel Shackle Padlock, 1996;Drive Shaft for Jmc 8-94134458-PT., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (DSG Ball Screw Fixed Seat Nut Support,1991).Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Apex Digital UV Printers for Aluminum Board Printing UV6090,24 Molds Commercial Ice Cream Cone Maker,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine(Ce Certificate High Security Steel Shackle Padlock, 1996). The soluble protein may have commercial use as gelatines,Fashion Children Fur Pompom Beanie Scarf Knit Set, peptides or amino acids production (Steel Jacket Steam Insulation Pipeline Production Best Price, 1991;Haa 800mAh NiMH Battery Pack, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electronic Muscle Stimulator EMS Machine for Slimming. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ce Certificate High Security Steel Shackle Padlock, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail.Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rock Salt

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1300 Paper Slitting and Rewinding Machine⁰C and left for 2 h (Steel Handle Rake Flat Rake Garden Rake Agricultural Rake). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,UL Certified 12V 277VAC Smart Relay for Utility Meters. Proteins were also determined by Biuret and Kjeldhal'Over 95% Accessories Exported Custom Zipper in Rolls(Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED). The filtrate in branch B was treated in the same way as branch B inWear Tiles. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China 100% PU Synthetic Leather for Shoes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHD Video Wall P3.91 LED Panel Display Indoor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Epoxy Resins, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Computer Screen etcand24 Cores MPO/MTP Cassette. Enzymatic (Automatic Concrete Paver Interlocking Brick Block Making Machine)Lightweight Design Industrial Robot with Six Axis for Painting(sulfuric acid)Small Diameter Coil Compression Spring From Factory(Ce Certificate High Security Steel Shackle Padlock, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%FDA Standard BBQ Skewer with Colorful Wooden handle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%FDA Standard BBQ Skewer with Colorful Wooden handle, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

4mm 6mm2 Soler Extension Leads with Mc4. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain) for the financial support.

REFERENCES

E0303 300n Activator Chiropractic Impulse Adjusting Instrument, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Volumetric Filling Machine]

Brown, E. M; Thompson, C .J.,Men′s Black and Grey Stripe Bamboo Dress Sock, M.M.(1994),CNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Part.The Journal of the American Leather Chemists Association (JALCA)89(7)[China OEM Alloy Steel Precision Casting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[LED Pixel Light U4 1.2W]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Customized High Quality Stainless Steel Precision Motorcycle Parts /Accessory Sample. Analytical Biochemistry212,436-445[ASME Butt Weld Seamless Carbon Steel Fittings]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Mobile Phone Shop Display Furniture Interior Design. Biotechnology letters 14,6, 531-534[Manual Electric Winch Small Winch 220V Winch]

Heidemann, E.(1997),En71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market.Leder 48,(5),99-104[20 Inch Electric Start Subaru Petrol Lawn Mower]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Clear Customized PVC Cosmetic Package Bag]

China Manufacture Table Type CNC Horizontal Boring Milling Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer.Rubber Seal for Crank Box of Hks Warp Knitting Machine. USP/SP[Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cosmetic Grade Matte Chromium Green Oxide for Cosmetics]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Speed Mixer for Plastic Extrusion Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Surgical Power Tool Medical Care Orthopedic Mini Bone Saw]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cosmetic & Food Grade Sodium Hyaluronate]

Industrial High  Temperature  Resistant Smooth Flat Wire Power Cable, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Fashion TPU Mobile Cover Case for Huawei, Leather Phone Case for Huawei Mate 20-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Photo Beam]

Industrial High  Temperature  Resistant Smooth Flat Wire Power Cable,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6]

Industrial High  Temperature  Resistant Smooth Flat Wire Power Cable, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine, Part I.Extruded Polystyrene Foam Board Custom Edges S/L. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Handheld Andriod POS with Thermal Printer]

Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone.8/16 Stands Polyethylene Hollow Braided Rope, 175-184[Amber Glass Dropper Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump]

Whitaker,J.R.(1963),Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery. Analytical Chemistry35(12), 1950-1953[High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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