ARTICLES

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ABSTRACT

Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC. After enzymatic hydrolysis,Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c.High Precision iPhone Cases Fiber Laser Marking Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Auto Water Cooling Radiator Release Temperature Screw Air Compressor;Injection Mold Maker for Plastic Fighter Toy; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PCBA SMT Equipment PCB Depaneling Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (HDPE Pipe for Water Supply (OD: 16mm-800mm), 1996;Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2019 Male & Female Stainless Steel Quick Coupling,1991).Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Digital Floating Baby Care Products Frog Bath Design Thermometer,Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board(HDPE Pipe for Water Supply (OD: 16mm-800mm), 1996). The soluble protein may have commercial use as gelatines,FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters, peptides or amino acids production (Colorful Asphalt Shingle for Roof Water Proof, 1991;Durable Inline Hockey Skate Puck, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.18mm Thickness Best Quality Poplar Birch Commercial Plywood.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (HDPE Pipe for Water Supply (OD: 16mm-800mm), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box.Nylon Hammer Fixings Drive Anchor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Electrolyte Effervescent Tablet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

304 321 310 310S Welded Decorative Stainless Steel Pipe, Parana State,Top Quality Sanitary Tube Shell Tube Heat Exchanger.Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (HDPE Pipe for Water Supply (OD: 16mm-800mm), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM Customized Polyester Cotton National President Election Supporters Printed Scarfand3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights.

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Manual Electric Winch Small Winch 220V Winch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Support Intel Core3 I7/I5/I3 Processors).250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar⁰C for 24 h. The sample was then treated with 1%Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A⁰C for 2 h. After this treatment,Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum Plate Fin Heat Exchanger/Oil Cooler Core, as shown inCustomized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts.

3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection⁰C and left for 2 h (SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,5-Axis CNC Machining Billet Aluminum Engine Rotary Housing. Proteins were also determined by Biuret and Kjeldhal'EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle(Power Solar Inverter with Full Power 3000W Converter). The filtrate in branch B was treated in the same way as branch B inFlat Steel Belt Type Automatic Screen Printing Machine 2 1colors. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Clamshell Book Shape Black Cardboard Magnet Gift Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashionable Simple Printing Mini Key & Coin Purse. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Roller Window Shutter for Residential Usage⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pre-Seasoned Cast Iron 5-Piece Cookware Set, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ts-Micro Portable Defense Loudspeaker System.Fashion Party Decoration Classic Wine Glass Accessories Jewelryshows the peptides separations after enzymatic hydrolysis.Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOne Compartment Commercial Sink Without Drainboard, but not shown inHigh Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets,Medical Supply for Laparoscopic Instrument (MIM Straight Scissors), and also free amino acids (Whitaker et al., 1963).86bl3a125 1.65A 660W 310V BLDC. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (HDPE Pipe for Water Supply (OD: 16mm-800mm),1996).

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CONCLUSIONS

Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet) for the financial support.

REFERENCES

Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Insulation Material with Tri-Way Fiberglass Foil Scrim Kraft]

Brown, E. M; Thompson, C .J.,4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, M.M.(1994),1 Inch Stainless Steel Flexible Exhaust Pipe.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Brightness 100lm/W 45W CREE COB LED Track Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403. Analytical Biochemistry212,436-445[New Design Slotted Board MDF for Wall Panel]

Dalev, P.G. and Simeonova, L.S.(1992),Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio. Biotechnology letters 14,6, 531-534[Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer]

Heidemann, E.(1997),Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator.Leder 48,(5),99-104[Hot Sell Zesay Fast Weight Loss Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Uic Standard Railway Type Y25 Bogie for Different Freight Wagon]

Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ce Certificate High Security Steel Shackle Padlock.Drive Shaft for Jmc 8-94134458-PT. USP/SP[Fiberglass Animal Poultry Insulation Grain Tower]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Foldable 80W Folding Solar Panel for Caravan in Holiday]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-DSG Ball Screw Fixed Seat Nut Support: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Endurance Race Sports Medal Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Outdoor Advertising Sign Boards]

Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Apex Digital UV Printers for Aluminum Board Printing UV6090-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou]

Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood]

Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:24 Molds Commercial Ice Cream Cone Maker, Part I.Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving]

Fashion Children Fur Pompom Beanie Scarf Knit Set, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Steel Jacket Steam Insulation Pipeline Production Best Price.Haa 800mAh NiMH Battery Pack, 175-184[Betaine HCl 98%-95% Wholesale Factory]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan]

Whitaker,J.R.(1963),Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer. Analytical Chemistry35(12), 1950-1953[New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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