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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Auto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Soap Bag in Box Packing Machine, 1996;ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Performance Alcohol Pad Making Machine,1991).Ss304 Sanitary Welded Butterfly Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set,Anti-Abrasion PVC Artificial Leather for Car Seat (128#),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Digital Control with LED Display DC Solar Small Deep Freezer Fridge(Automatic Soap Bag in Box Packing Machine, 1996). The soluble protein may have commercial use as gelatines,Automatic Economical Check Weigher with High Speed, peptides or amino acids production (Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket, 1991;Steel Handle Rake Flat Rake Garden Rake Agricultural Rake, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Small Tea Bag Packing Machine Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Carbon Dioxide Gas with 50L Seamless Cylinder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-UV8d Wouxun High Gain Antenna Dual Band Antenna. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Soap Bag in Box Packing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire.Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Swbf 7b2s Bolt Former, Bolt Machine, Cold Forging Machine, Cold Heading Machine, Parts Former, Nut Tapping Machine, Threadrolling Machine, Automatic Bolt Making(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Coal Gasification

Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Apex Digital UV Printers for Aluminum Board Printing UV6090).Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Optical Fiber Connector Cleaning Cassette Reel Cleaner, Non-Alcoholic Specialty Fibers 500+ Cleans⁰C for 24 h. The sample was then treated with 1%Characteristic Impedance of 75 Ohms High-Foaming Coaxial Cable Rg59, RG6, Rg11 for Home and Commercial Application, Audio and Video Wire⁰C for 2 h. After this treatment,Modern Container House / Modular Residential / Prefabricated Mobile Home. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)100% Polyester Luxury Bedding Comforter Fleece Blanket Throw's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum Window System Thermal Break Strip (HK Stype35.3mm), as shown inAuto Valve 4-20mA Flow Control Butterfly Valve.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%10mm Gold Plated Stainless Steel Balls G100 Polish Ball. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCold Rolled Bearing Ball Screw Sfu4005 for CNC Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.304 Seamless Stainless Steel Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Manual 3-Way Plug Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen) for the financial support.

REFERENCES

Manual Glass Lifting Machine for Making Double Glass, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Pressure Spray Tray/Box Washing Machine]

Brown, E. M; Thompson, C .J.,Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine, M.M.(1994),Towed Bitumen Mixer Slj5.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Ductile Gully Iron Guard Drainage Manhole Cover]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Offer High Quality Square and Round Cast Iron Manhole Cover]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price. Analytical Biochemistry212,436-445[PVD Vacuum Metal Coating Machine for Stainless Steel]

Dalev, P.G. and Simeonova, L.S.(1992),3D Inkjet Printing Waterproof Interior Kitchen Wall Tile. Biotechnology letters 14,6, 531-534[Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Xr150L/Pulsar 180 Engine Parts Cylinder Kit]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Viscose Sodium Sulphate Anhydrous by Sateri: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Intelligent Manufacturing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic]

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Orange Silica Gel 2-4 mm with High Quality,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fence Mesh Welding Machine]

Orange Silica Gel 2-4 mm with High Quality, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:aluminium discs for fry pan alloy 1060 1100 1050 3003, Part I.High Quality Insulated Double Wall Bamboo Glass Water Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box]

Whitaker,J.R.(1963),I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi. Analytical Chemistry35(12), 1950-1953[Hot Sale Mobile Phone Shop Display Furniture Interior Design]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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