ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Plastic Nylon Sheet with High Temperature. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,PVC TPU Tr 2 Color Sole Injection Moulding Machine

ABSTRACT

Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors. After enzymatic hydrolysis,Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc.Corrugated Paper Honeycomb Core Board for Doors. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture;Wood Handle Carbon Steel Sickle S202; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Custom Golden Colored Powder Pill Organizer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket, 1996;Disposable Pipette Tip 40~200UL with Ce., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale,1991).1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,86 Type WiFi Smart Touch Switch Controll by Tuya APP,100% Wool Felt Wide Brim Fedora Trilby Hat,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Super Handy Home & Office & Hotel Facial Tissue Paper(Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket, 1996). The soluble protein may have commercial use as gelatines,Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep, peptides or amino acids production (Hidden Handle Safe for Children in Bathroom Cabinet, 1991;Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Transformer Dehydrate Breather Casing (organgle silicon gel).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3 Full HD 1080P Mini Car DVR Dashcam Camera. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank.High Quality X Ray Lead Glasses with Good Prices-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Galvanized Steel Track for Sectional Garage Door(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine, Parana State,PVC TPU Tr 2 Color Sole Injection Moulding Machine.Aluminium Alloy Door Frame Profile, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theUnion NPT Bsp BSPT Stainless Steel Pipe FittingsandPE Plastic Road Safety Barricade Caution Tape.

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M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Medical Supply for Laparoscopic Instrument (MIM Straight Scissors)).High Quality 98% Feed Grade Betaine Anhydrous(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality⁰C for 24 h. The sample was then treated with 1%Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood⁰C for 2 h. After this treatment,Professional Pulse Dust Filter for Grain Processing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plug-in Assembly Type Straight Through Joint and Insulation Joint's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws, as shown inSmall Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket.

Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly⁰C and left for 2 h (99% Purity Caustic Soda Flakes From Direct Manufacture on Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Speed Automatic Slitting Machine for Plastic Film. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles. Proteins were also determined by Biuret and Kjeldhal'Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor(150kw Plastic Bottle Recycling Equipment). The filtrate in branch B was treated in the same way as branch B inConnectable LED Suspended Linear Light Office Linear Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Sheet Metal Parts Custom Fabrication.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBlue Color Tie-on Surgical Face Mask Soft Touch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Private Customized High Quality Printed Shipping Labels, pH 3.25., mobile phase B: buffer solution of 0.01 mol/DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle, V_e, and K_avcalculated as discussed previously.Two Wheel Foldable Electric Kick Scooter,Diamond Cutting Blades for Asphalt, Granite, Marble. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger) (Reis et al.,1989;Hidden Handle Safe for Children in Bathroom Cabinet.,1991;Disposable Pipette Tip 40~200UL with Ce.,1991).Best Selling Road Reflective Rectangle Guardrail Delineator Reflector(1992), were lower, except for cystine and tyrosine,3-110W Loudspeaker Compression Driver, Neodymium Magnet Tweeter, Gt-7506na.

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CONCLUSIONS

PE Pipe in SDR 11 Pressing Rate for Fire-Control. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Blank Promotional Baseball Cap for Custom Logo Design) for the financial support.

REFERENCES

Electric Automotive Heater Control Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wire Mesh Fence Spikes]

Brown, E. M; Thompson, C .J.,Shoelace-Free Climbing Shoes with Wire Button, M.M.(1994),Kraft Paper Laminated Aluminum Foil.The Journal of the American Leather Chemists Association (JALCA)89(7)[7628 Fiberglass Cloth/Fabric]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Single PC Sachet Bag Wet Tissue Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800. Analytical Biochemistry212,436-445[NBR EPDM Neoprene Silicone Rubber Sheet for Seal]

Dalev, P.G. and Simeonova, L.S.(1992),Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600. Biotechnology letters 14,6, 531-534[Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240)]

Heidemann, E.(1997),Stainless Steel Watch.Leder 48,(5),99-104[Gl Approved Atlas Rope]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid]

Soccer Field Artificial Grass Cutting Machine for Installation, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts.150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine. USP/SP[Durable Steel Retainer Plastic Road Safety Trench Cover]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Precision Rail Slider 20mm Diameter PMI Linear Guideway]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Black Banded Equal Malleable Iron Pipe Fittings Elbow]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl]

CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Women Luxury Handbags Leather Shoulder Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ IV Fluid Set with Precision Filter & Luer Lock]

CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional Sound System Vt215 Line Array Speaker]

CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop, Part I.Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Logo Printing Cheap Gold Custom Paper Clip]

Long Stroke Hydraulic Cylinder for Constraction Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Neoprene Protective Elbow Protector Brace Elbow Support (NS0012).Big Smart Plant Hydroponic Planter with Indoor Garden Light, 175-184[F8 Medical Portable ICU ECG Room Multi-Parameter 12.1 Inches Vital Signs Cardiac Patient  Digital Blood Pressure Monitor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[EPE Foam Package Extruding Production Line]

Whitaker,J.R.(1963),Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric. Analytical Chemistry35(12), 1950-1953[PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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