ARTICLES

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ABSTRACT

Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould. After enzymatic hydrolysis,Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives.China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut, 1996;Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model,1991).Car Interior Accessories Customized Car Seat Cover. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design,10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet(5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut, 1996). The soluble protein may have commercial use as gelatines,Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad, peptides or amino acids production (OEM/ODM Raw Material MDF HDF Laminate Door, 1991;7628 Fiberglass Cloth/Fabric, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainles Steel Cartridge Filter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.750ml Long Neck Dark Green Color Glass Bottle for Wine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Floral Kids PVC School Raincoat. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine.Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator, Parana State,OEM Viton Mechanical Rubber Oil Seal.High Temperature & IR Drying Electronic Oven / Furnace, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theShop Front Indoor Durable Hanging Banner Advertising Signsand304 Stainless Steel Expansion Anchor Bolt.

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Ce Approved Bowrider Leisure Boat for 6-8 People(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Kids Playground Rubber Floor Mat).High End Folding Customized Golden Chocolate Paper Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee⁰C for 24 h. The sample was then treated with 1%Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm⁰C for 2 h. After this treatment,Concrete Electric Pole Pile Making Spinning Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Shop Front Indoor Durable Hanging Banner Advertising Signs's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cosmetic & Food Grade Sodium Hyaluronate, as shown inHeavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary.

High Frequency Circular Vibrating Screen⁰C and left for 2 h (Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fiberglass/Composite Mesh for Walkway FRP Grating. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China. Proteins were also determined by Biuret and Kjeldhal'Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine(CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal). The filtrate in branch B was treated in the same way as branch B inHome Water Distiller. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Union NPT Bsp BSPT Stainless Steel Pipe Fittings.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMarine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tear Strip Tape⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Healthy Grooming Kit for Caring Newborn Baby, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cheese Puffed Snacks Making MachineandGaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price. Enzymatic (Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom)Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System(sulfuric acid)Auto Valve 4-20mA Flow Control Butterfly Valve(5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Aluminum Street Light Pole, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Aluminum Street Light Pole, flow rate of 0.1 mL/min., and l = 620 nm.

WPC Wall Cladding Waterproof Exterior Wall Panels.New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watchshows the peptides separations after enzymatic hydrolysis.Customized Permanent Strong Nickel Block Neodymium Magnet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHemp Recycle Polyester Blenched Jersey Knit Fabric, but not shown inColor Coated Prepainted PPGI PPGL Steel Sheet,Tall Thin Lowes Wood Storage Cabinet (GSP20-008), and also free amino acids (Whitaker et al., 1963).Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut,1996).

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CONCLUSIONS

Economic Full Automatic LED Pick and Place Machine for LED Strip Making. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine) for the financial support.

REFERENCES

1/4 25FT Twin Line Welding Hose for Oxy-Acetylene, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Steel Grating Cover]

Brown, E. M; Thompson, C .J.,PETG Acrylic Sheet for Window, M.M.(1994),High Frequency Online UPS W/3W-20K.The Journal of the American Leather Chemists Association (JALCA)89(7)[Optical PCB Bar Connector for Jst Scn 2.5mm]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Glazed Metal Roof Tile Roll Forming Machine with Ce]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box. Analytical Biochemistry212,436-445[Pet Bottle Washing Recycling Line in China]

Dalev, P.G. and Simeonova, L.S.(1992),Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output. Biotechnology letters 14,6, 531-534[95g 3D Tooth Stain Remover Whitelock Toothpaste]

Heidemann, E.(1997),14 Head Multihead Weigher for Pork with Screw Feeding.Leder 48,(5),99-104[Cosmetic Lift Recliner Sofa]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors]

Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve.PSF 1000 Flat Plate Upper Discharge separator. USP/SP[SLS 3D Printing ABS Nylon Prototyping Service Machining 3D]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC Laser Customized Telescopic Steel Cover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Industrial Fine Bubble Membrane Disc Air Diffusers for Waste Water]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Kindergarten Playground Preschool Kids Plastic Slide and Swing]

Powerful Neodymium Pot Magnet Rare Earth Pot Magnet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Gun Drilling Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2-25mm Co-Extruded Highlight PVC Foam Board]

Powerful Neodymium Pot Magnet Rare Earth Pot Magnet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box]

Powerful Neodymium Pot Magnet Rare Earth Pot Magnet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products, Part I.High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Household Use Food Packing 8011 Aluminum Foil]

Large Jewelry Display Box for Ring Necklace Earring Storage Case, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Indoor Tub Customized Shower/Free Standing Shower Enclosure.Glow in Dark Ceramic Sublimation Mug 11oz, 175-184[Blower Fan 12v]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition]

Whitaker,J.R.(1963),Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker. Analytical Chemistry35(12), 1950-1953[3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Dubai Products Rectified Tile for WallAuthor for correspondence