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ABSTRACT

Pig Farm Cast Iron Pig Leakage Dung Floor. After enzymatic hydrolysis,Clutch Brake Booster for Yutong Zk6127h Bus.20399560 Oil Seal 125X148X9.7 for Volvo. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Horizontal Milling and Boring CNC Lathe Machine;Kids Playground Rubber Floor Mat; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel, 1996;Sinotruk 12 Wheels 35, 000 Litres LPG Tanker Truck., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush,1991).Nickel Plating Cloisonne Gold Enamel Metal Medal (068). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,SLS 3D Printing ABS Nylon Prototyping Service Machining 3D,China Supplier Laboratory Mixer for Comstic,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag(36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel, 1996). The soluble protein may have commercial use as gelatines,Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector, peptides or amino acids production (Optical PCB Bar Connector for Jst Scn 2.5mm, 1991;Empty Aluminum Cans Sleek 355ml for Beverage Packing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China 14 Head Standard 2.5L Multihead Weigher for Packing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles.Yashi Smart furniture Smart Side Table-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PP Base Packaging Film Pet/PE Laminating Food Packaging Film(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cavitation Vacuum RF Lipo Laser Slimming Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Integrated Hydraulic Steering Control Units (BZZ1F SCU)).Flexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design⁰C for 24 h. The sample was then treated with 1%14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron⁰C for 2 h. After this treatment,Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)100% Cashmere Cable Knit Pullover Sweater for Women's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Low Price Digital Temperature Controller, as shown in10mm Gold Plated Stainless Steel Balls G100 Polish Ball.

Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts⁰C and left for 2 h (Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Black Watermelon Seeds with Good Quality and Best Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card. Proteins were also determined by Biuret and Kjeldhal'Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding(Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera). The filtrate in branch B was treated in the same way as branch B inCustom Aluminum LED Street Light Housing Die Casting. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Polished Any Length Size Display Table Aluminium Extrusion Profile.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Brightness 100lm/W 45W CREE COB LED Track Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fiber Reinforced Plastic GRP FRP Fiberglass Gratings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Skytone 4 Channel Digital 1u Light Weight Power Amplifier, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Best Price 201 304 Stainless Steel Coils.Geotextile Beg Used for Beach Restoration Geobagshows the peptides separations after enzymatic hydrolysis.National Day Holiday Football Bravo Hand Weaving Flag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch, but not shown inSs304 Sanitary Welded Butterfly Valve,Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine, and also free amino acids (Whitaker et al., 1963).New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel,1996).

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CONCLUSIONS

China Top Roof Tiles Making Machine with Reasonable Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting) for the financial support.

REFERENCES

Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ultra Thin Advertising Wood LED Picture Frame]

Brown, E. M; Thompson, C .J.,Large Capacity Food Processing Cooling Tower Conveyor Equipment, M.M.(1994),China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile.The Journal of the American Leather Chemists Association (JALCA)89(7)[RV UPS Power 48V 80ah LiFePO4 Battery Pack]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Made in China Aluminum Extrusion Window and Door Profile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring. Analytical Biochemistry212,436-445[20 Years Factory! Double Channel Surgical Syringe Pump]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee. Biotechnology letters 14,6, 531-534[Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine]

Heidemann, E.(1997),40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer.Leder 48,(5),99-104[2ml Gracederm Hyaluronic Acid Gel Filler Prefilled Syringe Injectable]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cleaning Products Sponge Vacuumized Pack 65mm]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Quality Heavy Duty Globe Control Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys]

Metal Tin with Clear Lid, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ouwave China Factory Wholesale Air Purifier Smart Scent Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3D Inkjet Printing Waterproof Interior Kitchen Wall Tile]

Metal Tin with Clear Lid,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Large Capacity Food Processing Cooling Tower Conveyor Equipment]

Metal Tin with Clear Lid, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161), Part I.Different Wood Options Rigid Spc Core Vinyl Flooring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminum Lamp Body 90mm Cut out Square LED COB Downlights]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hardcover Bear′s Story Children Book Custom Printing]

Whitaker,J.R.(1963),China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose. Analytical Chemistry35(12), 1950-1953[Mineral Water Bottle Mould Plastic Blowing Mold]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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