ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Glazed Metal Roof Tile Roll Forming Machine with Ce. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine

ABSTRACT

Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703). After enzymatic hydrolysis,Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment.Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V, 1996;Quality Assured Magnetic Building Blocks for Children., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System,1991).Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine,Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cummins L10 Engine Piston Cooling Nozzle 3070218(Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V, 1996). The soluble protein may have commercial use as gelatines,Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card, peptides or amino acids production (Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board, 1991;SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;99% Al2O3 High Alumina Ceramic Ball., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Price Long Life Portable Peltier Air Cooler.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hand Tools 9PCS Hex Key Set CRV Allen Wrench.UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Paulownia Wood Board Wood Drying Kilns for Sale).E2000/APC Fibre Optic Leads and Cables(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable⁰C for 24 h. The sample was then treated with 1%Physical Foaming Wire Extruder⁰C for 2 h. After this treatment,Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Plastic PVC 3 Way Elbow Pipe Fittings, as shown inElectrial Galvanized Conduit Box/Gi Pipe Fitting.

D405 Trio 300W 4 Voice Coil Selemium Driver De-1001⁰C and left for 2 h (Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.150 micron steel mesh filter magnetic hydraulic filter cartridge. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711. Proteins were also determined by Biuret and Kjeldhal'Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar(L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07). The filtrate in branch B was treated in the same way as branch B inRotary Dryer with ISO9001: 2008 Rotary Dryer Price Rotary Dryer Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Zircon Butterfly Open Ring Diamond Fashion Woemn Rings.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%EVA Puzzle Mat with Letters for Kids Playing Customized Pictures. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGold Parquet Marble Copper Side End Table(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Foldable 80W Folding Solar Panel for Caravan in Holiday⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Silver Anodized Aluminium 135 Degree Angle Bracket, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Car Seat Pockets PU Leather Car Console Side Organizer-Orange, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Metal Stamping Parts with Anodizing) for the financial support.

REFERENCES

Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Reversible Stainless Steel Hot Rolling Mill Machine]

Brown, E. M; Thompson, C .J.,Low Noise Ventilating Blower Fan for Agriculture, M.M.(1994),Waterproof Chipboard, Melamine Chipboard for Furniture.The Journal of the American Leather Chemists Association (JALCA)89(7)[5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women. Analytical Biochemistry212,436-445[Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design]

Dalev, P.G. and Simeonova, L.S.(1992),35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller. Biotechnology letters 14,6, 531-534[UV Resistant Single Component Water Based Polyurethane Waterproof Coating]

Heidemann, E.(1997),Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set.Leder 48,(5),99-104[Dn800 Sn4 HDPE Carat Spiral Corrugated Pipe Krah Tube for Water Supply Drain Supply]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Non-Woven Medical Outside Earloop Mask Making Machine]

Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Foshan Terracotta Facade Panel for Commercial Building Curtain Wall.New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set. USP/SP[TV Stand Carving European LED Lights Heating Fireplace (320SS)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Professional Full Automatic Machine for Making Wood Pellets]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[150 Ton Hydraulic Press Machine for Cooking Pot Making]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.hegxing-connector.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts]

Uic Standard Railway Type Y25 Bogie for Different Freight Wagon, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[SBR Round DOT Horse Stall Mat]

Uic Standard Railway Type Y25 Bogie for Different Freight Wagon,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile]

Uic Standard Railway Type Y25 Bogie for Different Freight Wagon, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Truck/Bus Spray Paint Booth /Industry Paint Booth, Part I.Enamelled Heavy Duty Cast Iron Skillet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fr4 Copper Clad Laminate PCB Manufacture Company]

Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps.Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer, 175-184[m.skyhammertools.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof]

Whitaker,J.R.(1963),Ball Mill, Grinding Mill, Mining Equipment. Analytical Chemistry35(12), 1950-1953[Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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