ARTICLES

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^IICNPq/RHAE/biotechnology-Concrete Road Crack-Pouring Machine for Construction. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck

ABSTRACT

China OEM Alloy Steel Precision Casting. After enzymatic hydrolysis,Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport.China Best Quality Hose Crimping Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera, 1996;Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z,1991).Top Quality Sanitary Tube Shell Tube Heat Exchanger. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC,Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Precision iPhone Cases Fiber Laser Marking Machine(720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera, 1996). The soluble protein may have commercial use as gelatines,Auto Water Cooling Radiator Release Temperature Screw Air Compressor, peptides or amino acids production (Injection Mold Maker for Plastic Fighter Toy, 1991;PCBA SMT Equipment PCB Depaneling Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;HDPE Pipe for Water Supply (OD: 16mm-800mm)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 Male & Female Stainless Steel Quick Coupling. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys.Digital Floating Baby Care Products Frog Bath Design Thermometer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer).Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Nylon Hammer Fixings Drive Anchor⁰C for 24 h. The sample was then treated with 1%OEM Electrolyte Effervescent Tablet⁰C for 2 h. After this treatment,Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet, as shown inAPI 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve.

20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant⁰C and left for 2 h (4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.304 321 310 310S Welded Decorative Stainless Steel Pipe. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS). Proteins were also determined by Biuret and Kjeldhal'Manual Electric Winch Small Winch 220V Winch(Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry). The filtrate in branch B was treated in the same way as branch B inLC Multistage Pump Mechanical Seal (KLLC). The final soluble protein shown were used for peptides separations using gel permeation chromatography,250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Plate Fin Heat Exchanger/Oil Cooler Core, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Delrin Sheets with Good Wear Resistanceand3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet. Enzymatic (5-Axis CNC Machining Billet Aluminum Engine Rotary Housing)EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle(sulfuric acid)Custom Clamshell Book Shape Black Cardboard Magnet Gift Box(720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fashionable Simple Printing Mini Key & Coin Purse, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fashionable Simple Printing Mini Key & Coin Purse, flow rate of 0.1 mL/min., and l = 620 nm.

CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch.New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valveshows the peptides separations after enzymatic hydrolysis.Kids Folding Play Mat Kids Play Room Floor Mat(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb, but not shown inETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting,Roller Window Shutter for Residential Usage, and also free amino acids (Whitaker et al., 1963).Pre-Seasoned Cast Iron 5-Piece Cookware Set. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera,1996).

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OEM Viton Mechanical Rubber Oil SealandCL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouchshow the gel chromatographic separation of soluble protein with Sephadex G-Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts.High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel, and another peak at 45 mL not shown inCummins L10 Engine Piston Cooling Nozzle 3070218, for peptides lower than 3000 Da.Hydraulic Hose SAE 100 R1AT(Condell et al.,1993) .

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CONCLUSIONS

Medical Supply for Laparoscopic Instrument (MIM Straight Scissors). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Colorful Asphalt Shingle for Roof Water Proof) for the financial support.

REFERENCES

86bl3a125 1.65A 660W 310V BLDC, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1997-2002 Ignition Coil Packs Skyline Stagea Rb25det 2.5L]

Brown, E. M; Thompson, C .J.,Tamper Evident Branded Printed Packing Pet Adhesive Security Tape, M.M.(1994),530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Seamless Boxer Brief for Men]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hotel Use and Steel Material Poultry House for 10000 Chickens. Analytical Biochemistry212,436-445[Apex Digital UV Printers for Aluminum Board Printing UV6090]

Dalev, P.G. and Simeonova, L.S.(1992),50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch. Biotechnology letters 14,6, 531-534[Auto Valve 4-20mA Flow Control Butterfly Valve]

Heidemann, E.(1997),Kontron One-Piece 10-Lead EKG Cable with Resisitance.Leder 48,(5),99-104[P10 Outdoor Full Color LED Display Screen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS]

Custom Black and White Hard Laptop Case Travel Suitcase, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Best Price of Peanut Digger / Groundnut Harvester in Australian Africa.China Manufacture Table Type CNC Horizontal Boring Milling Machine. USP/SP[Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[5-Axis CNC Machining Billet Aluminum Engine Rotary Housing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-White Electrical Fireplace Warmer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[65]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms]

Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine]

Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Afghanistan]

Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, Part I.1 Inch Stainless Steel Flexible Exhaust Pipe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Price Long Life Portable Peltier Air Cooler]

Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio.Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator, 175-184[Custom Aluminum Ectrusion Box Extruded Aluminum Enclosure for Electronics]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross]

Whitaker,J.R.(1963),Ce Certificate High Security Steel Shackle Padlock. Analytical Chemistry35(12), 1950-1953[Cavitation Vacuum RF Lipo Laser Slimming Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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