ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Grade Nitrous Oxide Gas / Laughing Gas / N2o. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor

ABSTRACT

Easy Soak off OEM 10ml Gel Nail Polish. After enzymatic hydrolysis,Cosmetic Case Box Packaging Makeup Case for Outdoor.Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Foil Container Making Machine, Pneumatic Punching Machine;LED Pixel Light U4 1.2W; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (15L/30L Vibratory Deburring Polishing Tumbler for Small Parts, 1996;10/16A EV Potable Charging Station Type 2 621962 Plug Cee., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794,1991).Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED,Low Noise Ventilating Blower Fan for Agriculture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waterproof Chipboard, Melamine Chipboard for Furniture(15L/30L Vibratory Deburring Polishing Tumbler for Small Parts, 1996). The soluble protein may have commercial use as gelatines,Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women, peptides or amino acids production (35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller, 1991;Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Foshan Terracotta Facade Panel for Commercial Building Curtain Wall., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (15L/30L Vibratory Deburring Polishing Tumbler for Small Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Uic Standard Railway Type Y25 Bogie for Different Freight Wagon.Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Truck/Bus Spray Paint Booth /Industry Paint Booth(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ball Mill, Grinding Mill, Mining Equipment, Parana State,Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor.Used Tyre Recycling Plant for Reclaimed Rubber Production, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (15L/30L Vibratory Deburring Polishing Tumbler for Small Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCNC Turned Aluminum Anodized Auto PartandHot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules.

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Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China 100% PU Synthetic Leather for Shoes).Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Medical Ce Approved Bp Machine Blood Pressure Monitor⁰C for 24 h. The sample was then treated with 1%Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910⁰C for 2 h. After this treatment,2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor, as shown in5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Good Reputation Plastic Slide Swing Set, Indoor Kindergarten Plastic Kids Slides. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Aluminum Laser Milling Machinery⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Porosity Zirconia Zro2 Foam Filter for Foundry, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher.PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg Chinashows the peptides separations after enzymatic hydrolysis.China Electric PCB Circuit Board with High Quality(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFake Fish Luminescent Tropical Fish Fish Tank Decoration, but not shown inOutdoor Portable Charcoal BBQ Grill Barbecue Camping Stove,OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts, and also free amino acids (Whitaker et al., 1963).Anodized Perforated 3D Facade Windows Screen for Commercial Building. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (15L/30L Vibratory Deburring Polishing Tumbler for Small Parts,1996).

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CONCLUSIONS

Professional Full Automatic Machine for Making Wood Pellets. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps) for the financial support.

REFERENCES

High Quality 20PCS Colorful Makeup Brush Set, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ductless Laboratory Fume Hood Safety machine Price]

Brown, E. M; Thompson, C .J.,Big size 420mm*420mm*460mm building plate,printing Rehabilitation of protective, 3D Printer used in rehabilitation therapy, M.M.(1994),PE Plastic Road Safety Barricade Caution Tape.The Journal of the American Leather Chemists Association (JALCA)89(7)[Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[300mm Stroke Linear Module Sliding Bearing for CNC System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Screw Barrel for PVC PPR Pipe Extrusion. Analytical Biochemistry212,436-445[Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock]

Dalev, P.G. and Simeonova, L.S.(1992),15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6. Biotechnology letters 14,6, 531-534[Supreme 524 Mv Sea Kayak]

Heidemann, E.(1997),Gold Open Metal Short Crocodile Wallet for Girl Women Lady.Leder 48,(5),99-104[Agricultural Equipment Parts Products Made in China]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Color Stretch Film for Pallet Wrap Use]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bathroom Ceramic Tiles]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Design Customized 100% Cotton Home Towel Bath Towel]

95g 3D Tooth Stain Remover Whitelock Toothpaste, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mini Outdoor Hydraulic Man Aluminum Lift]

95g 3D Tooth Stain Remover Whitelock Toothpaste,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[16oz Square Shape Colors Glass Mason Jar with Embossed Design]

95g 3D Tooth Stain Remover Whitelock Toothpaste, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Al/Pet/Al Double Sides for Aluminium Flexible Air Duct, Part I.European Standards 100A 24V Electrical Relay for Green Energy. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Private Customized High Quality Printed Shipping Labels]

Whitaker,J.R.(1963),Made in China Aluminum Extrusion Window and Door Profile. Analytical Chemistry35(12), 1950-1953[Good Quality CZ Purlin One Machine Roll Forming Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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