ARTICLES

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ABSTRACT

Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet. After enzymatic hydrolysis,Aluminum Foil Container Making Machine, Pneumatic Punching Machine.LED Pixel Light U4 1.2W. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing;15L/30L Vibratory Deburring Polishing Tumbler for Small Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.10/16A EV Potable Charging Station Type 2 621962 Plug Cee, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794, 1996;Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED,1991).Low Noise Ventilating Blower Fan for Agriculture. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Waterproof Chipboard, Melamine Chipboard for Furniture,Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller(Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794, 1996). The soluble protein may have commercial use as gelatines,Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set, peptides or amino acids production (Foshan Terracotta Facade Panel for Commercial Building Curtain Wall, 1991;New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Uic Standard Railway Type Y25 Bogie for Different Freight Wagon.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Truck/Bus Spray Paint Booth /Industry Paint Booth.Enamelled Heavy Duty Cast Iron Skillet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c, Parana State,Cosmetic Case Box Packaging Makeup Case for Outdoor.Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theUV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car CoverandHydrafacial Water Cleaning Skin Peel Facial Beauty Machine.

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Medical Ce Approved Bp Machine Blood Pressure Monitor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)).Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery⁰C for 24 h. The sample was then treated with 1%Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish⁰C for 2 h. After this treatment,Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Jieshuai 3 in 1 USB Cable, Fast Charging Data Cable, Universal Micro USB Cable, Lightning Cable, Type C Cable, Suitable Apple iPhone and Android Charge's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mini Electric Chain Saw Cutting Machine Price, as shown inHot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck.

A2 Grade Fireproof Customized Color ACP Sheet⁰C and left for 2 h ( Kitchen Floor Mat). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ce Approved Cheap Medical Disposable Electrical Infusion Pump. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fashion Party Decoration Classic Wine Glass Accessories Jewelry. Proteins were also determined by Biuret and Kjeldhal'Good Reputation Plastic Slide Swing Set, Indoor Kindergarten Plastic Kids Slides(5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive). The filtrate in branch B was treated in the same way as branch B inSanta Cutlery Sets. The final soluble protein shown were used for peptides separations using gel permeation chromatography,32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Galvanized Steel Barbed Wire Rope 8mm. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC Aluminum Laser Milling Machinery(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Big size 420mm*420mm*460mm building plate,printing Rehabilitation of protective, 3D Printer used in rehabilitation therapy. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ball Mill, Grinding Mill, Mining Equipment) for the financial support.

REFERENCES

PE Plastic Road Safety Barricade Caution Tape, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ess Inverter]

Brown, E. M; Thompson, C .J.,Screw Barrel for PVC PPR Pipe Extrusion, M.M.(1994),15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6.The Journal of the American Leather Chemists Association (JALCA)89(7)[Telescopic Slideway Steel Protection Accordion Cover]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Galvanized Steel Barbed Wire Rope 8mm]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Gold Open Metal Short Crocodile Wallet for Girl Women Lady. Analytical Biochemistry212,436-445[Double Wine Carrier Bag Gift Box Tote]

Dalev, P.G. and Simeonova, L.S.(1992),6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor. Biotechnology letters 14,6, 531-534[Plug-in Assembly Type Straight Through Joint and Insulation Joint]

Heidemann, E.(1997),Pond Self Priming Booster suction  Water Pump with Pressure Sensor.Leder 48,(5),99-104[Self Seal Poly Mailing Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable.95g 3D Tooth Stain Remover Whitelock Toothpaste. USP/SP[304 Seamless Stainless Steel Tube]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Canvas Casual Men Shoes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory For Double Usb Portcar Charger - 12V 150W Modified Sine Wave Inverter - Ligao]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[One Step HBV 5 in 1 Rapid Test]

Al/Pet/Al Double Sides for Aluminium Flexible Air Duct, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),European Standards 100A 24V Electrical Relay for Green Energy-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Kraft Paper Laminated Aluminum Foil]

Al/Pet/Al Double Sides for Aluminium Flexible Air Duct,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)]

Al/Pet/Al Double Sides for Aluminium Flexible Air Duct, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal, Part I.Canvas Shoulder Weekend Shopping Big Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts]

High Visuable Reflective Tape for Vehicle Road Safety, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Made in China Aluminum Extrusion Window and Door Profile.Industrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc, 175-184[L-carnitine Green Tee Capsule]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator]

Whitaker,J.R.(1963),Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump. Analytical Chemistry35(12), 1950-1953[Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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