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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Pig Farm Cast Iron Pig Leakage Dung Floor. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Clutch Brake Booster for Yutong Zk6127h Bus

ABSTRACT

20399560 Oil Seal 125X148X9.7 for Volvo. After enzymatic hydrolysis,Horizontal Milling and Boring CNC Lathe Machine.Kids Playground Rubber Floor Mat. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sinotruk 12 Wheels 35, 000 Litres LPG Tanker Truck, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush, 1996;Nickel Plating Cloisonne Gold Enamel Metal Medal (068)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SLS 3D Printing ABS Nylon Prototyping Service Machining 3D,1991).China Supplier Laboratory Mixer for Comstic. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag,Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Optical PCB Bar Connector for Jst Scn 2.5mm(All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush, 1996). The soluble protein may have commercial use as gelatines,Empty Aluminum Cans Sleek 355ml for Beverage Packing, peptides or amino acids production (4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes, 1991;China 14 Head Standard 2.5L Multihead Weigher for Packing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Yashi Smart furniture Smart Side Table. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PP Base Packaging Film Pet/PE Laminating Food Packaging Film.Wholesale Professional Indoor&Outdoor Trampoline Park-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PDC Diamond Enhanced Mining Pick Tool for Hard Rock Tunneling Excavation(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head).14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP⁰C for 24 h. The sample was then treated with 1%100% Cashmere Cable Knit Pullover Sweater for Women⁰C for 2 h. After this treatment,Low Price Digital Temperature Controller. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Black Watermelon Seeds with Good Quality and Best Price, as shown inOrange Silica Gel 2-4 mm with High Quality.

125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card⁰C and left for 2 h (Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Polished Any Length Size Display Table Aluminium Extrusion Profile. Proteins were also determined by Biuret and Kjeldhal'High Brightness 100lm/W 45W CREE COB LED Track Light(Hans Custom Manufactured Smoothness Zipper Waterproof). The filtrate in branch B was treated in the same way as branch B inBinocular Digital Microscope Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFiber Reinforced Plastic GRP FRP Fiberglass Gratings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Skytone 4 Channel Digital 1u Light Weight Power Amplifier⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Dongfeng 4X2 10000L Water Spinkling Tank Transport TruckandLED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp. Enzymatic (Mu Optical Fiber Connectors for Cable Assembly)Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning(sulfuric acid)Best Price 201 304 Stainless Steel Coils(All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%National Day Holiday Football Bravo Hand Weaving Flag, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%National Day Holiday Football Bravo Hand Weaving Flag, flow rate of 0.1 mL/min., and l = 620 nm.

Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine.Galvanized Steel Track for Sectional Garage Doorshows the peptides separations after enzymatic hydrolysis.New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFireproof WPC Indoor Decorative Wall Panel, but not shown in52cc Petrol Brush Cutter for DIY Garden Care,2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men, and also free amino acids (Whitaker et al., 1963).Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush,1996).

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CONCLUSIONS

Large Capacity Food Processing Cooling Tower Conveyor Equipment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (50d Cationic Spandex Stretch Fabric for Garment) for the financial support.

REFERENCES

China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[RFID Blocking Man Purse Credit Card Case Holder Genuine Leather Men Short Wallet]

Brown, E. M; Thompson, C .J.,Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring, M.M.(1994),Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee.The Journal of the American Leather Chemists Association (JALCA)89(7)[South Africa AC Power Cord Plug with C5 Connector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Precision Mini Laser Cutting System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer. Analytical Biochemistry212,436-445[Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen]

Dalev, P.G. and Simeonova, L.S.(1992),Pillow Case Automatic Sewing Machine Bc901. Biotechnology letters 14,6, 531-534[Smiley Canvas School Backpack for Teenagers Girls]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Canvas Shoulder Weekend Shopping Big Bag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Large Jewelry Display Box for Ring Necklace Earring Storage Case]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ouwave China Factory Wholesale Air Purifier Smart Scent Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Car Rotating Platform Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Grade Nitrous Oxide Gas / Laughing Gas / N2o]

Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Different Wood Options Rigid Spc Core Vinyl Flooring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[White Color Premium Quality Disposable Plastic PVC Apron]

Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluminum Road Stud]

Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Bucket Segment Bolt 5p7665, Part I.Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener]

Straight Seam Galvanized Pipe High Frequency Tube Welding Mill, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose.Flow Packing Machine for Bread, Soap, Biscuit, Chocolate, 175-184[Low Frequency Pure Sine Wave Inverter 12V 220V 2000W]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Design Disposable Trocars for Laparoscopic Surgery]

Whitaker,J.R.(1963),Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal. Analytical Chemistry35(12), 1950-1953[Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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