ARTICLES

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ABSTRACT

Stainles Steel Cartridge Filter. After enzymatic hydrolysis,750ml Long Neck Dark Green Color Glass Bottle for Wine.Floral Kids PVC School Raincoat. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (78mm Aluminum Gold Color Plastic Jar Screw Cap, 1996;Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine,1991).0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator,High Temperature & IR Drying Electronic Oven / Furnace,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ce Approved Bowrider Leisure Boat for 6-8 People(78mm Aluminum Gold Color Plastic Jar Screw Cap, 1996). The soluble protein may have commercial use as gelatines,High End Folding Customized Golden Chocolate Paper Box, peptides or amino acids production (HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee, 1991;Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Concrete Electric Pole Pile Making Spinning Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Shop Front Indoor Durable Hanging Banner Advertising Signs.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cosmetic & Food Grade Sodium Hyaluronate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (78mm Aluminum Gold Color Plastic Jar Screw Cap, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Frequency Circular Vibrating Screen.Fiberglass/Composite Mesh for Walkway FRP Grating-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch, Parana State,7628 Fiberglass Cloth/Fabric.Tear Strip Tape, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (78mm Aluminum Gold Color Plastic Jar Screw Cap, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePiston Step Combination Seal/Seal PTFE +Polyester Guide Ring GstandChina Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25.

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Healthy Grooming Kit for Caring Newborn Baby(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment).Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price⁰C for 24 h. The sample was then treated with 1%Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom⁰C for 2 h. After this treatment,Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Auto Valve 4-20mA Flow Control Butterfly Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Aluminum Street Light Pole, as shown in2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal.

WPC Wall Cladding Waterproof Exterior Wall Panels⁰C and left for 2 h (High Efficiency Pineapple Canning Plant). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Permanent Strong Nickel Block Neodymium Magnet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tall Thin Lowes Wood Storage Cabinet (GSP20-008). Proteins were also determined by Biuret and Kjeldhal'Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M)(Top Quality 99.95% Molybdenum Square Plates). The filtrate in branch B was treated in the same way as branch B inPP Picnic Folding Table Heat-Insulating Box Picnic Table with Chairs. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel LED Underground Light, Ground Lighting IP66.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avK Series Helical Bevel Reduction Gearbox Motor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mini Outdoor Hydraulic Man Aluminum Lift, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Fashion Women Clothes Fashion Yaga Pants, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Glow in Dark Ceramic Sublimation Mug 11oz. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip) for the financial support.

REFERENCES

Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Anti Slip Glass Beads]

Brown, E. M; Thompson, C .J.,Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve, M.M.(1994),22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PVC Shrink Sleeve Bottle Label for Can Packing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Supermarket Combined Island Chest Freezer Commercial Refrigerator. Analytical Biochemistry212,436-445[OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 Male & Female Stainless Steel Quick Coupling]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Coil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Concrete Road Crack-Pouring Machine for Construction: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Free POM Custom Knit Acrylic Beanie Caps]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wave Ball]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CNC Maching Parts for Autos]

Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China OEM Alloy Steel Precision Casting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Truck/Bus Spray Paint Booth /Industry Paint Booth]

Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack]

Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport, Part I.China Best Quality Hose Crimping Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide]

1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Galvanized Metal Welded PVC Coated Wire Mesh Fence.Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand, 175-184[m.kseidon.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts]

Whitaker,J.R.(1963),720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera. Analytical Chemistry35(12), 1950-1953[CNC Aluminum Laser Milling Machinery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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