ARTICLES

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ABSTRACT

China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile. After enzymatic hydrolysis,Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring.Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Lamp Body 90mm Cut out Square LED COB Downlights, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange, 1996;Metal Tin with Clear Lid., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ouwave China Factory Wholesale Air Purifier Smart Scent Machine,1991).Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Different Wood Options Rigid Spc Core Vinyl Flooring,High Quality Bucket Segment Bolt 5p7665,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales(Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange, 1996). The soluble protein may have commercial use as gelatines,Straight Seam Galvanized Pipe High Frequency Tube Welding Mill, peptides or amino acids production (China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose, 1991;Flow Packing Machine for Bread, Soap, Biscuit, Chocolate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Outdoor LED Decorative Street Linear Wall Lighting.Good Quanlity of Ue319an20z Hydraulic Oil Filter Element-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Universal Manual Turret Milling Machine(Mill X6336)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wood Handle Carbon Steel Sickle S202. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Custom Golden Colored Powder Pill Organizer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Disposable Pipette Tip 40~200UL with Ce⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Aluminium Alloy Door Frame Profile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Proper Price Good Quality 10ml 20ml Airless Pump Bottle) for the financial support.

REFERENCES

M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[WPC Spc Flooring Accessory]

Brown, E. M; Thompson, C .J.,High Quality 98% Feed Grade Betaine Anhydrous, M.M.(1994),Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Custom Forged Auto Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood. Analytical Biochemistry212,436-445[High Dust Holding Capacity HEPA Filter with Separator]

Dalev, P.G. and Simeonova, L.S.(1992),Professional Pulse Dust Filter for Grain Processing. Biotechnology letters 14,6, 531-534[Korea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi]

Heidemann, E.(1997),Plug-in Assembly Type Straight Through Joint and Insulation Joint.Leder 48,(5),99-104[gjmach-chn.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ cUL Certificate]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Good Reputation Plastic Slide Swing Set]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Speed Automatic Slitting Machine for Plastic Film: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cheap Topmac Brand Mobile Concrete Mixing Plant]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[pcd diamond cup wheel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone]

China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[A36 Galvanized Building Steel Structure Column H Steel Column]

China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Low Price Fashion Unique Plastic Zipper]

China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Sheet Metal Parts Custom Fabrication, Part I.Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Zoo-Tunes Portable Wireless Mini Character Speakers]

Blue Color Tie-on Surgical Face Mask Soft Touch, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine.Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak, 175-184[1.0um 3um 5.0 Micron Glass Fiber Membrane Filter Paper for Air Filtration]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Price of Custom Brands Granite Sink for Home]

Whitaker,J.R.(1963),Private Customized High Quality Printed Shipping Labels. Analytical Chemistry35(12), 1950-1953[Wholesale Fashion Women Clothes Fashion Yaga Pants]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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