ARTICLES

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ABSTRACT

Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241). After enzymatic hydrolysis,Fiber Reinforced Plastic GRP FRP Fiberglass Gratings.Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp, 1996;Mu Optical Fiber Connectors for Cable Assembly., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning,1991).Best Price 201 304 Stainless Steel Coils. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,National Day Holiday Football Bravo Hand Weaving Flag,Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function(LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp, 1996). The soluble protein may have commercial use as gelatines,2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men, peptides or amino acids production (Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015, 1991;OEM Customized Logo Print Polyester Nylon Gym Sport Blue Drawstring Backpack Bag Supplier, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Garden Irrigation Spray Micro Sprinkler Controller., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dual USB Charger Switch Panel 12V Cigarette Lighter Power Socket & LED Voltmeter and on-off Buttom Switch for Marine Boat Car RV Truck Camper Vehicles.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Free POM Custom Knit Acrylic Beanie Caps.Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Top Roof Tiles Making Machine with Reasonable Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Pillow Case Automatic Sewing Machine Bc901(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture).Aluminum Lamp Body 90mm Cut out Square LED COB Downlights(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange⁰C for 24 h. The sample was then treated with 1%Metal Tin with Clear Lid⁰C for 2 h. After this treatment,Ouwave China Factory Wholesale Air Purifier Smart Scent Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Different Wood Options Rigid Spc Core Vinyl Flooring, as shown in150ml Round Aromatherapy Diffuser Glass Bottle.

High Quality Bucket Segment Bolt 5p7665⁰C and left for 2 h (Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Straight Seam Galvanized Pipe High Frequency Tube Welding Mill. Proteins were also determined by Biuret and Kjeldhal'China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose(CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts). The filtrate in branch B was treated in the same way as branch B inCustom Basketball Jersey. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Flow Packing Machine for Bread, Soap, Biscuit, Chocolate.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPractical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor LED Decorative Street Linear Wall Lighting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Good Quanlity of Ue319an20z Hydraulic Oil Filter Element, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Universal Manual Turret Milling Machine(Mill X6336), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fireproof WPC Indoor Decorative Wall Panel.White Electrical Fireplace Warmershows the peptides separations after enzymatic hydrolysis.Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts, but not shown inDeep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking,3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall, and also free amino acids (Whitaker et al., 1963).High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp,1996).

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Micropore PP Pleated Cartridge Filter 10 20 30 40 InchandExperienced Construction Steel Structure Building Designshow the gel chromatographic separation of soluble protein with Sephadex G-CNC Machinery Aluminum and ABS Mock up Prototypes.Bsp 60° Male Cone Seat Carbon Steel Fittings, and another peak at 45 mL not shown inFactory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal, for peptides lower than 3000 Da.Soccer Field Artificial Grass Cutting Machine for Installation(Condell et al.,1993) .

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Plastic Nylon Sheet with High Temperature, V_e, and K_avcalculated as discussed previously.PVC TPU Tr 2 Color Sole Injection Moulding Machine,Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies) (Reis et al.,1989;Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015.,1991;Mu Optical Fiber Connectors for Cable Assembly.,1991).Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc(1992), were lower, except for cystine and tyrosine,Corrugated Paper Honeycomb Core Board for Doors.

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CONCLUSIONS

Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile) for the financial support.

REFERENCES

Wood Handle Carbon Steel Sickle S202, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Furniture Use Pre Gi Tube]

Brown, E. M; Thompson, C .J.,High Quality Custom Golden Colored Powder Pill Organizer, M.M.(1994),Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket.The Journal of the American Leather Chemists Association (JALCA)89(7)[M10 3D Solid Non-Woven Folded Mask Body Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Gold Parquet Marble Copper Side End Table]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Disposable Pipette Tip 40~200UL with Ce. Analytical Biochemistry212,436-445[Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables]

Dalev, P.G. and Simeonova, L.S.(1992),Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale. Biotechnology letters 14,6, 531-534[Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve]

Heidemann, E.(1997),1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids.Leder 48,(5),99-104[180 Degree Cameras]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Super Handy Home & Office & Hotel Facial Tissue Paper: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aoi Machine Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport]

Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hidden Handle Safe for Children in Bathroom Cabinet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor]

Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts]

Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale, Part I.Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Big Smart Plant Hydroponic Planter with Indoor Garden Light]

Transformer Dehydrate Breather Casing (organgle silicon gel), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3 Full HD 1080P Mini Car DVR Dashcam Camera.Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank, 175-184[Punch Type Hose Clamp]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube]

Whitaker,J.R.(1963),High Quality X Ray Lead Glasses with Good Prices. Analytical Chemistry35(12), 1950-1953[4-20mA PT100 Temperature Transmitter (PCT202)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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