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ABSTRACT

10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys. After enzymatic hydrolysis,Funny Kids Soft Play Maze Big Slide Indoor Soft Playground.Large Prototype CNC Transmission Assembly Tractor Machinery Part. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mini Recorder Sound Box for Musical Box & Plush Toys, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fake Fish Luminescent Tropical Fish Fish Tank Decoration, 1996;Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Pressure Die Casting Aluminum Die Casting Gear Box Housing,1991).Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove,CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Small Portable Air Water Purifier with Disinfection and Detoxification(Fake Fish Luminescent Tropical Fish Fish Tank Decoration, 1996). The soluble protein may have commercial use as gelatines,Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX, peptides or amino acids production (1850mm*80mm Stable Power Laser Tube, 1991;White Color Premium Quality Disposable Plastic PVC Apron, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Galvanized Bar Grating., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ring Die Bio Organic Fertilizer Pellet Mill Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Dipped Galvanized Deer Fence From Factory. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fake Fish Luminescent Tropical Fish Fish Tank Decoration, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce.Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cheap Customized Aluminium Sand Casting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality RV Electric Seating Recliner, Parana State,Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate.2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fake Fish Luminescent Tropical Fish Fish Tank Decoration, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBeauty Equipment Skin Care Machine PDT-LED Light Therapy MachineandGlobal Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp.

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100% Hand-Painted Canvas Oil Painting Wall Art for Trees(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (30t/H RO Reverse Osmosis System Water Treatment Plant).Factort Price Cattle Beef Slaughtering Equipment for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Supreme 524 Mv Sea Kayak⁰C for 24 h. The sample was then treated with 1%API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve⁰C for 2 h. After this treatment,1-40mm Thickness and 1.22m, 1.56m Size PVC Celuka Board Manufacturer for Cabinets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Definition Outdoor Video Advertising LED Billboard's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm, as shown inTop Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector.

304 Stainless Steel Expansion Anchor Bolt⁰C and left for 2 h (HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Small Plug-in Insect Killer Mosquito Killing Lamp. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Customize PU Leather Hardcover Notebook with Window. Proteins were also determined by Biuret and Kjeldhal'Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11)(Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft). The filtrate in branch B was treated in the same way as branch B inSilver Mirror Glass High Reflective Polished Mirror Finish Aluminum Coil. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Coil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

LED Alloy Aluminum Extrusion Profiles Advertising Accessories for LightingandBest Quality White Color Electric Curtain Anti UV Self-Adhesive Pdlc Smart Film. Enzymatic (Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars)Xtsky Automotive Wheel Hub Bearing (DAC37720037)(sulfuric acid)Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle(Fake Fish Luminescent Tropical Fish Fish Tank Decoration, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear, flow rate of 0.1 mL/min., and l = 620 nm.

FTTH Optical Network Terminal for Gpon ONU and CATV.White Latex Paper Yellow/White Stearate Coated Abrasive Papershows the peptides separations after enzymatic hydrolysis.OEM Customized Polyester Cotton National President Election Supporters Printed Scarf(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inW Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass, but not shown inHigh Speed Rope Weaving Machine,D8tc High Performance 3-Electrode Motorcycle Spark Plug, and also free amino acids (Whitaker et al., 1963).Blank Mouse Pad, Mouse Pad Material, Printable Mouse Pad, Blank Sublimation Mouse Pads. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fake Fish Luminescent Tropical Fish Fish Tank Decoration,1996).

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OEM Custom Design Print Cotton Microfiber Travel Beach Towels ManufacturerandQuality Printed Cigarette Tipping Papershow the gel chromatographic separation of soluble protein with Sephadex G-Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging.Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag, and another peak at 45 mL not shown inFan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c, for peptides lower than 3000 Da.19m Mobile LED Display Billboard for Advertising Screens(Condell et al.,1993) .

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Nice Appearance Brass Metal Sheet Stamping, V_e, and K_avcalculated as discussed previously.Hot Sale Starter Motor Testing Equipment for Truck, Bus,Home Machine 2019 New Commercial Treadmill Gym Equipment. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Industrial Bedsheet Folding Machine (ZD-3000)) (Reis et al.,1989;1850mm*80mm Stable Power Laser Tube.,1991;Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine.,1991).New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic(1992), were lower, except for cystine and tyrosine,Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line.

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CONCLUSIONS

Clothing Hard Tag Security Tag for Supermarket/Retail Store. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China) for the financial support.

REFERENCES

150 Ton Hydraulic Press Machine for Cooking Pot Making, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[tnhg8]

Brown, E. M; Thompson, C .J.,Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl, M.M.(1994),Hot Sale Flat New Design Ladies Winter Warm Shoes.The Journal of the American Leather Chemists Association (JALCA)89(7)[National Day Holiday Football Bravo Hand Weaving Flag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves. Analytical Biochemistry212,436-445[Hand Tools 9PCS Hex Key Set CRV Allen Wrench]

Dalev, P.G. and Simeonova, L.S.(1992),OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer. Biotechnology letters 14,6, 531-534[3phase DC to AC Variable Frequency Drive Water Solar PV Inverter]

Heidemann, E.(1997),China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine.Leder 48,(5),99-104[Pipe Fitting Stainless Steel/Carbon Steel A105 Forged/Flat/Slip-on/Orifice/ Lap Joint/Soket Weld/Blind /Butt Welding Neck Flanges]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Precision Ce Radial Drill Machine Z3040]

High Speed Rope Weaving Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal.IP67 High-Ending Industrial Socket / Connector. USP/SP[Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[304/304L 2b Stainless Steel Sheet and Steel Plate]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mc4 Solar PV Connector TUV Approval: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Silicone Folding Cup]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size]

PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[1 Inch Stainless Steel Flexible Exhaust Pipe]

PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Visuable Reflective Tape for Vehicle Road Safety]

PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Over Band Round Type Electro Magnetic Separator for Tramp Iron, Part I.Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Permanent Strong Nickel Block Neodymium Magnet]

Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer.Bgf-800 Pet Dry Laminating Machine, 175-184[Wedding Tin Box Chocolate Candy Tins with Window]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve]

Whitaker,J.R.(1963),Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse. Analytical Chemistry35(12), 1950-1953[Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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