ARTICLES

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ABSTRACT

OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps. After enzymatic hydrolysis,10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts.Mini Outdoor Hydraulic Man Aluminum Lift. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Economic Full Automatic LED Pick and Place Machine for LED Strip Making, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1/4 25FT Twin Line Welding Hose for Oxy-Acetylene, 1996;PETG Acrylic Sheet for Window., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Frequency Online UPS W/3W-20K,1991).Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output,14 Head Multihead Weigher for Pork with Screw Feeding,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve(1/4 25FT Twin Line Welding Hose for Oxy-Acetylene, 1996). The soluble protein may have commercial use as gelatines,PSF 1000 Flat Plate Upper Discharge separator, peptides or amino acids production (Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart, 1991;Powerful Neodymium Pot Magnet Rare Earth Pot Magnet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC Gun Drilling Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1/4 25FT Twin Line Welding Hose for Oxy-Acetylene, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Large Jewelry Display Box for Ring Necklace Earring Storage Case.Indoor Tub Customized Shower/Free Standing Shower Enclosure-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Glow in Dark Ceramic Sublimation Mug 11oz(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price, Parana State,K Series Helical Bevel Reduction Gearbox Motor.High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (1/4 25FT Twin Line Welding Hose for Oxy-Acetylene, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEmpty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for WineandCustom Silver Anodized Aluminium 135 Degree Angle Bracket.

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Clip on Wire Shelving Data Strip(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Drum Rotator).Custom Black and White Hard Laptop Case Travel Suitcase(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Concrete Road Crack-Pouring Machine for Construction⁰C for 24 h. The sample was then treated with 1%Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck⁰C for 2 h. After this treatment,China OEM Alloy Steel Precision Casting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Best Quality Hose Crimping Machine, as shown in Lid Press Machine.

1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor⁰C and left for 2 h (Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Galvanized Metal Welded PVC Coated Wire Mesh Fence. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand. Proteins were also determined by Biuret and Kjeldhal'720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera(Metal Unfolding Grocery Push Carts). The filtrate in branch B was treated in the same way as branch B inHigh Pressure Pump for Waterjet Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTop Quality Sanitary Tube Shell Tube Heat Exchanger(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Precision iPhone Cases Fiber Laser Marking Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Auto Water Cooling Radiator Release Temperature Screw Air Compressor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Bwb-306 Custom Printing Outdoor Sports WristbandandInjection Mold Maker for Plastic Fighter Toy. Enzymatic (PCBA SMT Equipment PCB Depaneling Machine)HDPE Pipe for Water Supply (OD: 16mm-800mm)(sulfuric acid)Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs(1/4 25FT Twin Line Welding Hose for Oxy-Acetylene, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2019 Male & Female Stainless Steel Quick Coupling, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2019 Male & Female Stainless Steel Quick Coupling, flow rate of 0.1 mL/min., and l = 620 nm.

Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys.Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supplyshows the peptides separations after enzymatic hydrolysis.Digital Floating Baby Care Products Frog Bath Design Thermometer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, but not shown inEasy Soak off OEM 10ml Gel Nail Polish,Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, and also free amino acids (Whitaker et al., 1963).Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1/4 25FT Twin Line Welding Hose for Oxy-Acetylene,1996).

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CONCLUSIONS

OEM Electrolyte Effervescent Tablet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine) for the financial support.

REFERENCES

Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1000l Ibc Blow Moulding Machine Suppliers for Sale]

Brown, E. M; Thompson, C .J.,6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter, M.M.(1994),Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet.The Journal of the American Leather Chemists Association (JALCA)89(7)[8/16 Stands Polyethylene Hollow Braided Rope]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China 100% PU Synthetic Leather for Shoes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant. Analytical Biochemistry212,436-445[5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter]

Dalev, P.G. and Simeonova, L.S.(1992),304 321 310 310S Welded Decorative Stainless Steel Pipe. Biotechnology letters 14,6, 531-534[Anti-Abrasion PVC Artificial Leather for Car Seat (128#)]

Heidemann, E.(1997),Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS).Leder 48,(5),99-104[High Quality Air Cooler Chiller Sintd for Plastic Injection Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts]

Stainless Steel LED Underground Light, Ground Lighting IP66, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Manual Electric Winch Small Winch 220V Winch.250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price. USP/SP[EV Charger for 120KW DC Fast Electric Car Charging Station]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fake Fish Luminescent Tropical Fish Fish Tank Decoration]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Tensile 8*19s Elevator Steel Wire Rope]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove]

Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing]

Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Zircon Butterfly Open Ring Diamond Fashion Woemn Rings]

Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part, Part I.Aluminum Plate Fin Heat Exchanger/Oil Cooler Core. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory]

3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet.5-Axis CNC Machining Billet Aluminum Engine Rotary Housing, 175-184[Ac/Dc Illuminated Switch]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line]

Whitaker,J.R.(1963),EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle. Analytical Chemistry35(12), 1950-1953[Anti-Theft EAS Hard Tag Tag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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