ARTICLES

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CarburetorEdivaldo E.Garcia^I; Cláudia C.M.Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{China OEM Alloy Steel Precision Casting}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Natural Clean Non- Ornamentate Decoration Material Wallpaper

ABSTRACT

98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder. After enzymatic hydrolysis,Super Soft 100% Viscose From Bamboo Bedding Set.Theme Jungle Plastic Stainless Steel Slide Outdoor Playground. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking;Updated Stylish Peristaltic Biogas Pump Gas Booster Pump; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.110V-240V LED Filament Bulb Globe Cafe Patio String Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Professional Sound System Vt215 Line Array Speaker, 1996;Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting,1991).Duct Evaporative Air Cooler for Warehouse Factory. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Micro Sprinkler for Water Saving Irrigation,Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High End L Shape Modular Sectional Sofa Patio Furniture Set Garden(Professional Sound System Vt215 Line Array Speaker, 1996). The soluble protein may have commercial use as gelatines,ABS Shell Plastic Injection Molding, peptides or amino acids production (Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press, 1991;450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manual Glass Lifting Machine for Making Double Glass., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Towed Bitumen Mixer Slj5. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Professional Sound System Vt215 Line Array Speaker, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price.3D Inkjet Printing Waterproof Interior Kitchen Wall Tile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI, Parana State,Natural Clean Non- Ornamentate Decoration Material Wallpaper.aluminium discs for fry pan alloy 1060 1100 1050 3003, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Professional Sound System Vt215 Line Array Speaker, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLED Pixel Light U4 1.2WandASME Butt Weld Seamless Carbon Steel Fittings.

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High Quality Insulated Double Wall Bamboo Glass Water Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Clear Customized PVC Cosmetic Package Bag).Aroma Reed Diffuser with Ratten Sticks for Home Fragrance(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Fashion Ladies Backpack PU Leather Backpack⁰C for 24 h. The sample was then treated with 1%Suction Filter Cylinder Match 48 Series Filter Brands⁰C for 2 h. After this treatment,I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)16oz Square Shape Colors Glass Mason Jar with Embossed Design's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer, as shown inWholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump.

Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms⁰C and left for 2 h (Cosmetic Grade Matte Chromium Green Oxide for Cosmetics). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.100ml Plastic E Liquid Vape Cig Juice Dropper Bottle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building. Proteins were also determined by Biuret and Kjeldhal'Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags(High Speed Mixer for Plastic Extrusion Line). The filtrate in branch B was treated in the same way as branch B inChemical White Powder Aluminium Sulphate Granular. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2-25mm Co-Extruded Highlight PVC Foam Board.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Engraving Machine Laser Marking for Assembly Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/nellcor non-oximax 7pin infant disposable sensor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cosmetic & Food Grade Sodium HyaluronateandPower Solar Inverter with Full Power 3000W Converter. Enzymatic (Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve)Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts(sulfuric acid)Zoo-Tunes Portable Wireless Mini Character Speakers(Professional Sound System Vt215 Line Array Speaker, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CF8m Air Actuated Valve Dn15, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CF8m Air Actuated Valve Dn15, flow rate of 0.1 mL/min., and l = 620 nm.

Rectangular Design Ceramic Small Glass Basin Sink for Bathroom. Photo Beamshows the peptides separations after enzymatic hydrolysis.Premium Baby Toddler Food Feeding Silicone Bowl with Lid(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6, but not shown inHandheld Andriod POS with Thermal Printer,Hardcover Bear′s Story Children Book Custom Printing, and also free amino acids (Whitaker et al., 1963).Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Professional Sound System Vt215 Line Array Speaker,1996).

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CONCLUSIONS

Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Viscose Sodium Sulphate Anhydrous by Sateri) for the financial support.

REFERENCES

Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Anti Aging Insulation Membrane with EVA Insulation Blanket for Roof Insulation]

Brown, E. M; Thompson, C .J.,Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine, M.M.(1994),One Compartment Commercial Sink Without Drainboard.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ge Holter Cable Snap Type Holter ECG Cable. Analytical Biochemistry212,436-445[UV8d Wouxun High Gain Antenna Dual Band Antenna]

Dalev, P.G. and Simeonova, L.S.(1992),Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5. Biotechnology letters 14,6, 531-534[Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch]

Heidemann, E.(1997),PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass.Leder 48,(5),99-104[4 Wheel Retractable Roller Skate Shoes]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality]

Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),65 Polyester 35 Cotton Fabric Camouflage Printed Fabric.Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine. USP/SP[Luxury Hotel Furniture Antique Design Marble Side Table]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Brass Floor Lamp Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets)]

OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rolling Shutter Door Hardwares, Link Pipe, Link Clip-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar]

OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Clip on Wire Shelving Data Strip]

OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine, Part I.China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241)]

FUJI Small Home Lift for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier.Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator, 175-184[Wholesale China Custom Metal Bronze Gold Plating Military Pilot Wings Pin Badge with Your Own Design]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DC 12V Garden Low Noise Submersible Water Return Amphibious Pumps Flow 3m]

Whitaker,J.R.(1963),Ce Jacket Heated Water Chocolate Holding Tank Manufacturer. Analytical Chemistry35(12), 1950-1953[22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Neodymium Magnet TweeterAuthor for correspondence