ARTICLES

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ABSTRACT

Plastic PVC 3 Way Elbow Pipe Fittings. After enzymatic hydrolysis,D405 Trio 300W 4 Voice Coil Selemium Driver De-1001.150 micron steel mesh filter magnetic hydraulic filter cartridge. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Zircon Butterfly Open Ring Diamond Fashion Woemn Rings, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (EVA Puzzle Mat with Letters for Kids Playing Customized Pictures, 1996;Gold Parquet Marble Copper Side End Table., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing,1991).Foldable 80W Folding Solar Panel for Caravan in Holiday. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Silver Anodized Aluminium 135 Degree Angle Bracket,Car Seat Pockets PU Leather Car Console Side Organizer-Orange,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale(EVA Puzzle Mat with Letters for Kids Playing Customized Pictures, 1996). The soluble protein may have commercial use as gelatines,Oxalic Acid for 99.6% Purity, peptides or amino acids production (Kids Waterproof Softshell Jacket with Brushed Fleece Inside, 1991;CNC Turned Aluminum Anodized Auto Part, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4-20mA PT100 Temperature Transmitter (PCT202)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Grade Nitrous Oxide Gas / Laughing Gas / N2o. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (EVA Puzzle Mat with Letters for Kids Playing Customized Pictures, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor.Easy Soak off OEM 10ml Gel Nail Polish-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cosmetic Case Box Packaging Makeup Case for Outdoor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Economical Check Weigher with High Speed).Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED⁰C for 24 h. The sample was then treated with 1%Low Noise Ventilating Blower Fan for Agriculture⁰C for 2 h. After this treatment,Waterproof Chipboard, Melamine Chipboard for Furniture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller, as shown in75 Cubic Meters Precast Concrete Batching Plant for Construction Building.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Truck/Bus Spray Paint Booth /Industry Paint Booth(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ce Approved Cheap Medical Disposable Electrical Infusion Pump, V_e, and K_avcalculated as discussed previously.Fashion Party Decoration Classic Wine Glass Accessories Jewelry,Good Reputation Plastic Slide Swing Set, Indoor Kindergarten Plastic Kids Slides. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Medical Supplier Non Woven 3ply Earloop Disposable Face Mask) (Reis et al.,1989;Kids Waterproof Softshell Jacket with Brushed Fleece Inside.,1991;Gold Parquet Marble Copper Side End Table.,1991).32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores(1992), were lower, except for cystine and tyrosine,Galvanized Steel Barbed Wire Rope 8mm.

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CONCLUSIONS

CNC Aluminum Laser Milling Machinery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (LED Pixel Light U4 1.2W) for the financial support.

REFERENCES

High Porosity Zirconia Zro2 Foam Filter for Foundry, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[70 Inch Tv Stand With Fireplace Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression, M.M.(1994),Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops.The Journal of the American Leather Chemists Association (JALCA)89(7)[Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids. Analytical Biochemistry212,436-445[Halogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type)]

Dalev, P.G. and Simeonova, L.S.(1992),Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844). Biotechnology letters 14,6, 531-534[Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse]

Heidemann, E.(1997),Antique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair.Leder 48,(5),99-104[Soft Sole Leather Baby Infant Toddler Kids]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Electric PCB Circuit Board with High Quality: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Light Weight Ohsas 18001 Certified Metal Arch Buildings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[ISO Industrial Precision Convection Electric Drying Oven Chamber]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Performance Alcohol Pad Making Machine]

OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Anodized Perforated 3D Facade Windows Screen for Commercial Building-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies]

OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Reusable CAT6 UTP RJ45 8P8C Modular Plug]

OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Full Cream Milk Powder Filling Machine (XFF-G), Part I.Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine]

Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale.Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat, 175-184[Automatic Tomato Ketchup Chili Sauce Shampoo Filling Packing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality]

Whitaker,J.R.(1963),Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment. Analytical Chemistry35(12), 1950-1953[Top Quality Sanitary Tube Shell Tube Heat Exchanger]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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