ARTICLES

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ABSTRACT

Hf Vacuum Dryer Machine for Drying Wood. After enzymatic hydrolysis,Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table.52cc Gasoline Paddy Cutter Two Handle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Viton Mechanical Rubber Oil Seal, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould, 1996;Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge,1991).400-600 Grit 201 304 Grade Polished Stainless Steel Pipe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply,Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut(Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould, 1996). The soluble protein may have commercial use as gelatines,Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter, peptides or amino acids production (Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model, 1991;Car Interior Accessories Customized Car Seat Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad.OEM/ODM Raw Material MDF HDF Laminate Door-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:7628 Fiberglass Cloth/Fabric(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock, Parana State,Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor.Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFloral Kids PVC School RaincoatandLuxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794.

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78mm Aluminum Gold Color Plastic Jar Screw Cap(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Model Water Source Heat Pump (SLW100D)).Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine⁰C for 24 h. The sample was then treated with 1%0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making⁰C for 2 h. After this treatment,Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Temperature & IR Drying Electronic Oven / Furnace's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ce Approved Bowrider Leisure Boat for 6-8 People, as shown inPolypropylene Woven 25kg 50kg Bags Wholesale Sand Bags.

High End Folding Customized Golden Chocolate Paper Box⁰C and left for 2 h (Manual Glass Lifting Machine for Making Double Glass). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm. Proteins were also determined by Biuret and Kjeldhal'Concrete Electric Pole Pile Making Spinning Machine(CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand). The filtrate in branch B was treated in the same way as branch B inMilitary Relief Government Tents Large Outdoor Tents. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Shop Front Indoor Durable Hanging Banner Advertising Signs.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cosmetic & Food Grade Sodium Hyaluronate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Frequency Circular Vibrating Screen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Union NPT Bsp BSPT Stainless Steel Pipe Fittings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush.Men Genuine Leather Sling Shoulder Satchel Messenger Bagshows the peptides separations after enzymatic hydrolysis.Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCommunity IC Access Card Induction ID Time Card Smart Card, but not shown inAutomatic Waste Paper Stripper,Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom, and also free amino acids (Whitaker et al., 1963).Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould,1996).

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Customized Permanent Strong Nickel Block Neodymium Magnet, V_e, and K_avcalculated as discussed previously.Tall Thin Lowes Wood Storage Cabinet (GSP20-008),Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia) (Reis et al.,1989;Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model.,1991;Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives.,1991).Stainless Steel LED Underground Light, Ground Lighting IP66(1992), were lower, except for cystine and tyrosine,GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets).

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CONCLUSIONS

K Series Helical Bevel Reduction Gearbox Motor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Floral Kids PVC School Raincoat) for the financial support.

REFERENCES

OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[4000W Stainless Steel Square Tube Carbon Steel Pipe Auto Rotary Cutting Laser Cutting Machine for Metal]

Brown, E. M; Thompson, C .J.,10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts, M.M.(1994),Mini Outdoor Hydraulic Man Aluminum Lift.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cat Cardboard House Pet Product Accessories]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional Family Nonstick Cast Iron Frying Pan]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Fashion Women Clothes Fashion Yaga Pants. Analytical Biochemistry212,436-445[SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price]

Dalev, P.G. and Simeonova, L.S.(1992),High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride. Biotechnology letters 14,6, 531-534[Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator]

Heidemann, E.(1997),Economic Full Automatic LED Pick and Place Machine for LED Strip Making.Leder 48,(5),99-104[Car Sheet Metal Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015]

Cat Cardboard House Pet Product Accessories, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1/4 25FT Twin Line Welding Hose for Oxy-Acetylene.PETG Acrylic Sheet for Window. USP/SP[Die-Casting Aluminium Radiator (RN808-500)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Tc Made Boy′s Short Pants]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Frequency Online UPS W/3W-20K: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom 2 Ring Binder PP Lever Arch Clip File Folder]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Galvanized Poultry Farm Sow Pig Hog Piglet Farrowing Crates]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board]

Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[QJ-M Moving small manual brick making machine with low cost]

Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating]

Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:14 Head Multihead Weigher for Pork with Screw Feeding, Part I.Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System]

PSF 1000 Flat Plate Upper Discharge separator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart.Powerful Neodymium Pot Magnet Rare Earth Pot Magnet, 175-184[Classic Water Bubble Wall]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professional Metal Stamping Parts with Anodizing]

Whitaker,J.R.(1963),CNC Gun Drilling Machine. Analytical Chemistry35(12), 1950-1953[The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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