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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-100ml Square Glass Diffuser Bottle with Cap. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers

ABSTRACT

Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts. After enzymatic hydrolysis,Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve.17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Good Quality OEM Stainless Steel Casting Rings, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet, 1996;Customizable Logo Plastic Safety Walking Reflectors Chinese Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle,1991).Flame Retardant FRP/GRP Round Pipe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Psa Oxygen Generator for Industry (ISO9001, CE),LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display(Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet, 1996). The soluble protein may have commercial use as gelatines,Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting, peptides or amino acids production (Custom small plastic printed box for keychain (PET box), 1991;Fence Mesh Welding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;20 Years Factory! Double Channel Surgical Syringe Pump., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Best Quality for Stretch Film Jumbo Roll.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Drum Type Magnetic Separator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰K-Pop Bts Baseball Hat Sport Anime Cap.Q32 Series Tumble Belt Shot Blasting Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Home Daily Tealight Glass Holder, Glass Candle Holder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Clutch Brake Booster for Yutong Zk6127h Bus).High Lumen Outdoor IP65 100W LED Flood Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality⁰C for 24 h. The sample was then treated with 1%Retail Supermarket Display Refrigerator Supermarket Beverage Showcase⁰C for 2 h. After this treatment,European Fashion Style Ladies Wrist Watch with Interchangeable Strap. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer, as shown inHorizontal Directional Drilling Machine SHD28.

Full Automatic Combination Weighing Packing Machine Jy-420A⁰C and left for 2 h (65 Polyester 35 Cotton Fabric Camouflage Printed Fabric). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tfl Blue Rubber Transmission Belts/Automotive Spare Part. Proteins were also determined by Biuret and Kjeldhal'1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China(0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer). The filtrate in branch B was treated in the same way as branch B inFlexible Water Hose 1/2 Inch X 1/2 Inch Push-to-Connect Braided Stainless Steel Water Connector Braided Hose (KX-005H). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Flat Wedge Wire Panel China Factory.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest Selling Hydraulic Small Truck with Crane 10 Ton(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Design Disposable Trocars for Laparoscopic Surgery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Disposable Medical Combined Bacterial Viral Hme Filter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine, V_e, and K_avcalculated as discussed previously.Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications,Cookies Cake Automatic Rotary Packing Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood) (Reis et al.,1989;Custom small plastic printed box for keychain (PET box).,1991;Customizable Logo Plastic Safety Walking Reflectors Chinese Factory.,1991).Building Material Quick Install Types of Scaffolding Couplers(1992), were lower, except for cystine and tyrosine,Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury.

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CONCLUSIONS

Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS) for the financial support.

REFERENCES

Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Two-Color Nylon Coated Blade and Double-Sided Printing]

Brown, E. M; Thompson, C .J.,Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt, M.M.(1994),PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth.The Journal of the American Leather Chemists Association (JALCA)89(7)[Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Silver Anodized Aluminium 135 Degree Angle Bracket]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092. Analytical Biochemistry212,436-445[Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep]

Dalev, P.G. and Simeonova, L.S.(1992),Mini Succulent Planters, Cactus Pots with Wooden Cement Pot. Biotechnology letters 14,6, 531-534[ Drum Rotator]

Heidemann, E.(1997),Folded Kitchen Towel Paper Wrapping Packaging Machine.Leder 48,(5),99-104[Bag Welding Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Lid Press Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Metal Unfolding Grocery Push Carts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Bwb-306 Custom Printing Outdoor Sports Wristband]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Yitai Die Making Birch Plywood Flat Die Mould Die Making]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply]

Hemp Recycle Polyester Blenched Jersey Knit Fabric, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW]

Hemp Recycle Polyester Blenched Jersey Knit Fabric,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Easy Soak off OEM 10ml Gel Nail Polish]

Hemp Recycle Polyester Blenched Jersey Knit Fabric, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule, Part I.3D Creative Design Metal Medal Promotion Gift of Sport. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Yoga Towel]

HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe.Composite Plate Dedicated Drop Hose Gear Metering Pump Price, 175-184[Professional Mould Maker and Plastic Injection Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom]

Whitaker,J.R.(1963),Brake Fluid Tank Container Hot Plate Plastic Welding Machine. Analytical Chemistry35(12), 1950-1953[Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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