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ABSTRACT

One Step HBV 5 in 1 Rapid Test. After enzymatic hydrolysis,Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp.Pig Farm Cast Iron Pig Leakage Dung Floor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Horizontal Milling and Boring CNC Lathe Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Kids Playground Rubber Floor Mat, 1996;100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel,1991).Sinotruk 12 Wheels 35, 000 Litres LPG Tanker Truck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush,Nickel Plating Cloisonne Gold Enamel Metal Medal (068),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,SLS 3D Printing ABS Nylon Prototyping Service Machining 3D(Kids Playground Rubber Floor Mat, 1996). The soluble protein may have commercial use as gelatines,China Supplier Laboratory Mixer for Comstic, peptides or amino acids production (Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag, 1991;Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Optical PCB Bar Connector for Jst Scn 2.5mm., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Empty Aluminum Cans Sleek 355ml for Beverage Packing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Kids Playground Rubber Floor Mat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China 14 Head Standard 2.5L Multihead Weigher for Packing Machine.OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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50d Cationic Spandex Stretch Fabric for Garment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Construction & Decoration ERW Square Steel Tube Manufacturer).Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cavitation Vacuum RF Lipo Laser Slimming Machine⁰C for 24 h. The sample was then treated with 1%Flexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm⁰C for 2 h. After this treatment,Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP, as shown inFiber Reinforced Plastic GRP FRP Fiberglass Gratings.

100% Cashmere Cable Knit Pullover Sweater for Women⁰C and left for 2 h (40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Low Price Digital Temperature Controller. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts. Proteins were also determined by Biuret and Kjeldhal'Black Watermelon Seeds with Good Quality and Best Price(SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock). The filtrate in branch B was treated in the same way as branch B inClassic Citizen COB Anti-Glare European LED Flush Mounted Downlight. The final soluble protein shown were used for peptides separations using gel permeation chromatography,125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPolished Any Length Size Display Table Aluminium Extrusion Profile(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fiber Reinforced Plastic GRP FRP Fiberglass Gratings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Mu Optical Fiber Connectors for Cable Assembly.Airtac Tubing Quick Connect Puick Coupling Pneumatic Fittingshows the peptides separations after enzymatic hydrolysis.Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHorizontal Milling and Boring CNC Lathe Machine, but not shown inCog Mono Round Display LCD Module,Best Price 201 304 Stainless Steel Coils, and also free amino acids (Whitaker et al., 1963).National Day Holiday Football Bravo Hand Weaving Flag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Kids Playground Rubber Floor Mat,1996).

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CONCLUSIONS

High Quality Free POM Custom Knit Acrylic Beanie Caps. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Professional Indoor&Outdoor Trampoline Park) for the financial support.

REFERENCES

Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[63 Amp Circuit Breaker Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,China Top Roof Tiles Making Machine with Reasonable Price, M.M.(1994),Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electric 100W Three Wheels Toy Car for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Large Capacity Food Processing Cooling Tower Conveyor Equipment. Analytical Biochemistry212,436-445[Digital Floating Baby Care Products Frog Bath Design Thermometer]

Dalev, P.G. and Simeonova, L.S.(1992),China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile. Biotechnology letters 14,6, 531-534[Pig Farm Cast Iron Pig Leakage Dung Floor]

Heidemann, E.(1997),Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring.Leder 48,(5),99-104[Fitness Apparel Box]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Boiler Room Ventilation Exhaust Fan]

Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee.40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer. USP/SP[China 14 Head Standard 2.5L Multihead Weigher for Packing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1300 Paper Slitting and Rewinding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pillow Case Automatic Sewing Machine Bc901: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality X Ray Lead Glasses with Good Prices]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Thread Valve With Lever Handle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts]

Aluminum Lamp Body 90mm Cut out Square LED COB Downlights, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine]

Aluminum Lamp Body 90mm Cut out Square LED COB Downlights,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp]

Aluminum Lamp Body 90mm Cut out Square LED COB Downlights, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Metal Tin with Clear Lid, Part I.Ouwave China Factory Wholesale Air Purifier Smart Scent Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[8FT Wood Log Debarker Machine for Plywood Machinery]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[30t/H RO Reverse Osmosis System Water Treatment Plant]

Whitaker,J.R.(1963),Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales. Analytical Chemistry35(12), 1950-1953[Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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