ARTICLES

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ABSTRACT

Psa Oxygen Generator for Industry (ISO9001, CE). After enzymatic hydrolysis,LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting.P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting;Custom small plastic printed box for keychain (PET box); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fence Mesh Welding Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (20 Years Factory! Double Channel Surgical Syringe Pump, 1996;Best Quality for Stretch Film Jumbo Roll., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Drum Type Magnetic Separator,1991).K-Pop Bts Baseball Hat Sport Anime Cap. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Q32 Series Tumble Belt Shot Blasting Machine,Home Daily Tealight Glass Holder, Glass Candle Holder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant(20 Years Factory! Double Channel Surgical Syringe Pump, 1996). The soluble protein may have commercial use as gelatines,Round Manufactory Non-Stick Silicone Stainless Dog Feeder, peptides or amino acids production (Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS, 1991;15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (20 Years Factory! Double Channel Surgical Syringe Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Lumen Outdoor IP65 100W LED Flood Light.Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Retail Supermarket Display Refrigerator Supermarket Beverage Showcase(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale).Flat Wedge Wire Panel China Factory(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625⁰C for 24 h. The sample was then treated with 1%Best Selling Hydraulic Small Truck with Crane 10 Ton⁰C for 2 h. After this treatment,Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Design Disposable Trocars for Laparoscopic Surgery, as shown inPopular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring.

Disposable Medical Combined Bacterial Viral Hme Filter⁰C and left for 2 h (300mm Stroke Linear Module Sliding Bearing for CNC System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System. Proteins were also determined by Biuret and Kjeldhal'3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb(Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock). The filtrate in branch B was treated in the same way as branch B inGorgeous Design and High Quality Printed PVC Card Business Cards. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Efficiency Pineapple Canning Plant.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Embossed Pattern PU Leather Classic Mens Wallet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Supra Brand Laundry Liquid Baby Formula⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cleaning Products Sponge Vacuumized Pack 65mm, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Supreme 524 Mv Sea KayakandMineral Water Bottle Mould Plastic Blowing Mold. Enzymatic (High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards)6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine(sulfuric acid)Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications(20 Years Factory! Double Channel Surgical Syringe Pump, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cookies Cake Automatic Rotary Packing Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cookies Cake Automatic Rotary Packing Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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Mini Succulent Planters, Cactus Pots with Wooden Cement Pot, V_e, and K_avcalculated as discussed previously.Folded Kitchen Towel Paper Wrapping Packaging Machine,Cheap Topmac Brand Mobile Concrete Mixing Plant. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Private Customized High Quality Printed Shipping Labels) (Reis et al.,1989;Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS.,1991;Best Quality for Stretch Film Jumbo Roll.,1991).Color Stretch Film for Pallet Wrap Use(1992), were lower, except for cystine and tyrosine,Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair.

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CONCLUSIONS

Hemp Recycle Polyester Blenched Jersey Knit Fabric. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer) for the financial support.

REFERENCES

Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[15X30mm Colombia/Peru/Chile/Bolivia PVC Trunking with Blue Tapepvc Trunking with Blue Tape / Electrical Cable Trunking / Trunking with Adhesive]

Brown, E. M; Thompson, C .J.,Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule, M.M.(1994),3D Creative Design Metal Medal Promotion Gift of Sport.The Journal of the American Leather Chemists Association (JALCA)89(7)[Premium Baby Toddler Food Feeding Silicone Bowl with Lid]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Density Graphite Crucible for Melting Metal]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line. Analytical Biochemistry212,436-445[Long Stroke Hydraulic Cylinder for Constraction Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe. Biotechnology letters 14,6, 531-534[Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper]

Heidemann, E.(1997),Composite Plate Dedicated Drop Hose Gear Metering Pump Price.Leder 48,(5),99-104[Fabric-Reinforced 4-Ply Rubber Sand Blast Hose Chinese Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Handcrank Miniature Musical Movement]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal]

Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Efficiency Pineapple Canning Plant]

Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Top Quality 99.95% Molybdenum Square Plates]

Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Puffed Core Filling Sanck Production Machine with Ce From China, Part I.86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Aluminum Street Light Pole]

Cummins Fuel Injector Kit (3076736 3095023), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5.Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch, 175-184[Child Seat Recreational Sea Kayak]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer]

Whitaker,J.R.(1963),Plastic Therm Forming Filling Sealing Machine, Lid Press Machine. Analytical Chemistry35(12), 1950-1953[European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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