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ABSTRACT

38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle. After enzymatic hydrolysis,China Supplier Dual Voltage Steam Curling Tongs (A129).High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;ASME Butt Weld Seamless Carbon Steel Fittings;Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Two Wheel Foldable Electric Kick Scooter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Diamond Cutting Blades for Asphalt, Granite, Marble, 1996;Best Selling Road Reflective Rectangle Guardrail Delineator Reflector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3-110W Loudspeaker Compression Driver, Neodymium Magnet Tweeter, Gt-7506na,1991).PE Pipe in SDR 11 Pressing Rate for Fire-Control. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Electric Automotive Heater Control Valve,Shoelace-Free Climbing Shoes with Wire Button,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Kraft Paper Laminated Aluminum Foil(Diamond Cutting Blades for Asphalt, Granite, Marble, 1996). The soluble protein may have commercial use as gelatines,China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800, peptides or amino acids production (Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600, 1991;Stainless Steel Watch, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Diamond Cutting Blades for Asphalt, Granite, Marble, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal.Women Luxury Handbags Leather Shoulder Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric, Parana State,Factory Professional Design Wing Shape Engraved Car Label Emblem.High Quality Anti-Corrosion Fiberglass Sheet and Roll, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Diamond Cutting Blades for Asphalt, Granite, Marble, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic SheetandHigh Capacity Vibrating Screen Manufacturer.

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Durable Steel Retainer Plastic Road Safety Trench Cover(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail).Industrial Low Temperature Water Cooled Scroll Water Chiller(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)⁰C for 24 h. The sample was then treated with 1%EV Charger for 120KW DC Fast Electric Car Charging Station⁰C for 2 h. After this treatment,Custom Velvet Jewelry Box Gift Box with Bowknots for Packing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode, as shown inPlastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A.

Aluminum Waterproof Folding Marquee Canopy Quick up Tent⁰C and left for 2 h (High Quality RV Electric Seating Recliner). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.4-20mA Current Sensor with Current Meter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tc Made Boy′s Short Pants. Proteins were also determined by Biuret and Kjeldhal'Light Weight Ohsas 18001 Certified Metal Arch Buildings(Reinforced Brass Coated Steel Wire for Hose Wire). The filtrate in branch B was treated in the same way as branch B inHigh Quality 64mm 50mm Oil Suction Industrial Hose High Pressure Wire Hydraulic Rubber Hose /High Pressure Hydraulic Rubber Hose Manuli Bntflex Semperit Hoses. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New 60W LED Beam Zoom Moving Head with Prism.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Maching Parts for Autos. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Pressure Pump for Waterjet Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PE Pipe in SDR 11 Pressing Rate for Fire-ControlandDie-Casting Aluminium Radiator (RN808-500). Enzymatic (Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL)Wholecut Oxford Plain Toe Black Antique Men Shoes(sulfuric acid)New Technology Carbon Cleaning Tools Auto Accessories(Diamond Cutting Blades for Asphalt, Granite, Marble, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Environmental Test Salt Spray Tester Price, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Environmental Test Salt Spray Tester Price, flow rate of 0.1 mL/min., and l = 620 nm.

Custom 2 Ring Binder PP Lever Arch Clip File Folder.Mini Electric Chain Saw Cutting Machine Priceshows the peptides separations after enzymatic hydrolysis.Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inManufacturing Plant Supply High Quality Aws E6013 Welding Electrode, but not shown inHot Sale Classic Golden Painted Rectangular Mirrored Dining Table,TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector, and also free amino acids (Whitaker et al., 1963).CNC Laser Customized Telescopic Steel Cover. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Diamond Cutting Blades for Asphalt, Granite, Marble,1996).

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304 Stainless Steel Expansion Anchor Boltand2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machieryshow the gel chromatographic separation of soluble protein with Sephadex G-High Precision Mini Laser Cutting System.Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong, and another peak at 45 mL not shown inKids Playground Rubber Floor Mat, for peptides lower than 3000 Da.1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195)(Condell et al.,1993) .

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One to One Order Following Strong Real Gold Thread, V_e, and K_avcalculated as discussed previously.New Design Paper Pyramid Memo Cube with Ribbon for Promotion,Plastic Medallion, Plastic Ceiling Rose. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line) (Reis et al.,1989;Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600.,1991;Best Selling Road Reflective Rectangle Guardrail Delineator Reflector.,1991).PP PE PVC Single Wall Corrugated Pipe Making Machine Line(1992), were lower, except for cystine and tyrosine,Lace Control Hiphugger Braless Latex Body Shaper L81187.

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CONCLUSIONS

Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Neoprene Protective Elbow Protector Brace Elbow Support (NS0012)) for the financial support.

REFERENCES

Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Brass High Pressure Auto Industry Pneumatic Rotary Joint Union]

Brown, E. M; Thompson, C .J.,Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine, M.M.(1994),Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile.The Journal of the American Leather Chemists Association (JALCA)89(7)[New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hemp Recycle Polyester Blenched Jersey Knit Fabric]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Centrifugal Casting Stainless Steel Furnace Tube. Analytical Biochemistry212,436-445[Color Coated Prepainted PPGI PPGL Steel Sheet]

Dalev, P.G. and Simeonova, L.S.(1992),Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System. Biotechnology letters 14,6, 531-534[Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag]

Heidemann, E.(1997),Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting.Leder 48,(5),99-104[Q37 Factory Price Hook Type Sand Blasting Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mini Succulent Planters]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wiskind Fireproof Hydrophobic Waterproof Rock Wool/Glass Wool Sandwich Panel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Low Price Digital Temperature Controller]

3phase DC to AC Variable Frequency Drive Water Solar PV Inverter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Quality Printed Cigarette Tipping Paper-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Motar Plastering Machine with Screw Pump]

3phase DC to AC Variable Frequency Drive Water Solar PV Inverter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Square Vibrating Sieve Machine]

3phase DC to AC Variable Frequency Drive Water Solar PV Inverter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:White Latex Paper Yellow/White Stearate Coated Abrasive Paper, Part I.Manufacturer Automotive CNC Machined Parts Checking Fixture Components. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Optical PCB Bar Connector for Jst Scn 2.5mm]

Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Telescopic Slideway Steel Protection Accordion Cover.Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes, 175-184[China Activated Alumina Catalyst Carrier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Glazed Metal Roof Tile Roll Forming Machine with Ce]

Whitaker,J.R.(1963),Cosmetic Grade Matte Chromium Green Oxide for Cosmetics. Analytical Chemistry35(12), 1950-1953[Pet Bottle Washing Recycling Line in China]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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