ARTICLES

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ABSTRACT

SBR Round DOT Horse Stall Mat, Rubber Stable Mat. After enzymatic hydrolysis,Customized Frame Mount Highway Pegs Enclosure Parts.Double Wine Carrier Bag Gift Box Tote. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.RF Coaxail BNC Male to BNC Male Plug Connector Adapter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Gt4b63 Can Seamer Machine, 1996;Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Glazed Metal Roof Tile Roll Forming Machine with Ce,1991).Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703),Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing(Gt4b63 Can Seamer Machine, 1996). The soluble protein may have commercial use as gelatines,Extruder Floating/Sinking Fish Feed Pellet Dryer, peptides or amino acids production (Custom Durable Silicone Rubber Seal Ring for Pressure Cooker, 1991;High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Quality Assured Magnetic Building Blocks for Children.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Gt4b63 Can Seamer Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary.Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Professional Family Nonstick Cast Iron Frying Pan).Hand Tools 9PCS Hex Key Set CRV Allen Wrench(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover⁰C for 24 h. The sample was then treated with 1%Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply⁰C for 2 h. After this treatment,Handheld Andriod POS with Thermal Printer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel French Earring Hook for Jewelry's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Professional Metal Stamping Parts with Anodizing, as shown inSMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPhysical Foaming Wire Extruder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic PVC 3 Way Elbow Pipe Fittings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/D405 Trio 300W 4 Voice Coil Selemium Driver De-1001, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Die-Casting Aluminium Radiator (RN808-500)and150 micron steel mesh filter magnetic hydraulic filter cartridge. Enzymatic (Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711)Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar(sulfuric acid)Zircon Butterfly Open Ring Diamond Fashion Woemn Rings(Gt4b63 Can Seamer Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%EVA Puzzle Mat with Letters for Kids Playing Customized Pictures, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%EVA Puzzle Mat with Letters for Kids Playing Customized Pictures, flow rate of 0.1 mL/min., and l = 620 nm.

Gold Parquet Marble Copper Side End Table.Tc Made Boy′s Short Pantsshows the peptides separations after enzymatic hydrolysis.Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom 2 Ring Binder PP Lever Arch Clip File Folder, but not shown inSolid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board,Foldable 80W Folding Solar Panel for Caravan in Holiday, and also free amino acids (Whitaker et al., 1963).Custom Silver Anodized Aluminium 135 Degree Angle Bracket. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Gt4b63 Can Seamer Machine,1996).

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Tec-Sieve Heavy-Duty Expanded Metal Catwalk GratingandType B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas Systemshow the gel chromatographic separation of soluble protein with Sephadex G-Car Seat Pockets PU Leather Car Console Side Organizer-Orange.New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale, and another peak at 45 mL not shown inProfessional Metal Stamping Parts with Anodizing, for peptides lower than 3000 Da.Oxalic Acid for 99.6% Purity(Condell et al.,1993) .

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Kids Waterproof Softshell Jacket with Brushed Fleece Inside, V_e, and K_avcalculated as discussed previously.CNC Turned Aluminum Anodized Auto Part,4-20mA PT100 Temperature Transmitter (PCT202). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Australia Standard Hot Sale 750ml Glass Wine Bottle) (Reis et al.,1989;Custom Durable Silicone Rubber Seal Ring for Pressure Cooker.,1991;Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror.,1991).ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross(1992), were lower, except for cystine and tyrosine,High Grade Nitrous Oxide Gas / Laughing Gas / N2o.

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CONCLUSIONS

Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board) for the financial support.

REFERENCES

Easy Soak off OEM 10ml Gel Nail Polish, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Crucible Heaters Silica Dioxide Weight]

Brown, E. M; Thompson, C .J.,Cosmetic Case Box Packaging Makeup Case for Outdoor, M.M.(1994),Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Large Span Steel Structrure for Warehouse From China]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Over 95% Accessories Exported Custom Zipper in Rolls]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminum Foil Container Making Machine, Pneumatic Punching Machine. Analytical Biochemistry212,436-445[High Efficiency Swimming Pool Air Source Water Heat Pump]

Dalev, P.G. and Simeonova, L.S.(1992),LED Pixel Light U4 1.2W. Biotechnology letters 14,6, 531-534[Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile]

Heidemann, E.(1997),Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing.Leder 48,(5),99-104[Dnv 2.7-1 Container Boat Shaped 13FT Offshore Waste Skip]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[L133pbd Common Rail Delphi Nozzle for Ford Mondeo]

New Condition Textured Peas Soy Protein Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),15L/30L Vibratory Deburring Polishing Tumbler for Small Parts.10/16A EV Potable Charging Station Type 2 621962 Plug Cee. USP/SP[Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Medical Ce Approved Bp Machine Blood Pressure Monitor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Best Die Cutting Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional Pulse Dust Filter for Grain Processing]

Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[50kVA Oil Immersed Transformer]

Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Men′s Black and Grey Stripe Bamboo Dress Sock]

Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Low Noise Ventilating Blower Fan for Agriculture, Part I.Waterproof Chipboard, Melamine Chipboard for Furniture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hexagonal Netting/ Gabion Box/ Gabion Basket]

Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller.Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set, 175-184[Armored Power Cable High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customization! Bdy380CD Horizontal Directional Drilling Machine]

Whitaker,J.R.(1963),Foshan Terracotta Facade Panel for Commercial Building Curtain Wall. Analytical Chemistry35(12), 1950-1953[Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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