ARTICLES

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ABSTRACT

LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp. After enzymatic hydrolysis,Mu Optical Fiber Connectors for Cable Assembly.Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Best Price 201 304 Stainless Steel Coils;National Day Holiday Football Bravo Hand Weaving Flag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function, 1996;2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015,1991).OEM Customized Logo Print Polyester Nylon Gym Sport Blue Drawstring Backpack Bag Supplier. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Garden Irrigation Spray Micro Sprinkler Controller,Dual USB Charger Switch Panel 12V Cigarette Lighter Power Socket & LED Voltmeter and on-off Buttom Switch for Marine Boat Car RV Truck Camper Vehicles,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030)(New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function, 1996). The soluble protein may have commercial use as gelatines,High Quality Free POM Custom Knit Acrylic Beanie Caps, peptides or amino acids production (Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric, 1991;China Top Roof Tiles Making Machine with Reasonable Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Large Capacity Food Processing Cooling Tower Conveyor Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring.Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Different Wood Options Rigid Spc Core Vinyl Flooring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Small Diameter Coil Compression Spring From Factory).High Quality Bucket Segment Bolt 5p7665(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales⁰C for 24 h. The sample was then treated with 1%Straight Seam Galvanized Pipe High Frequency Tube Welding Mill⁰C for 2 h. After this treatment,China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Flow Packing Machine for Bread, Soap, Biscuit, Chocolate's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal, as shown inStainless Steel French Earring Hook for Jewelry.

Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)⁰C and left for 2 h (PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor LED Decorative Street Linear Wall Lighting. Proteins were also determined by Biuret and Kjeldhal'Good Quanlity of Ue319an20z Hydraulic Oil Filter Element(Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector). The filtrate in branch B was treated in the same way as branch B inTv Safety Strap. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Universal Manual Turret Milling Machine(Mill X6336).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHexagonal Netting/ Gabion Box/ Gabion Basket(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fire Retardant Reflective Aluminium Foil Woven for Insulation Material, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fireproof WPC Indoor Decorative Wall Panel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Soccer Field Artificial Grass Cutting Machine for Installation.Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109shows the peptides separations after enzymatic hydrolysis.Plastic Nylon Sheet with High Temperature(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFTTH Optical Network Terminal for Gpon ONU and CATV, but not shown inElectric Automotive Heater Control Valve,PVC TPU Tr 2 Color Sole Injection Moulding Machine, and also free amino acids (Whitaker et al., 1963).Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function,1996).

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Wood Handle Carbon Steel Sickle S202, V_e, and K_avcalculated as discussed previously.High Quality Custom Golden Colored Powder Pill Organizer,Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (OEM/ODM Raw Material MDF HDF Laminate Door) (Reis et al.,1989;Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric.,1991;2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men.,1991).Disposable Pipette Tip 40~200UL with Ce(1992), were lower, except for cystine and tyrosine,Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale.

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CONCLUSIONS

1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange) for the financial support.

REFERENCES

86 Type WiFi Smart Touch Switch Controll by Tuya APP, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Precision FRP Compression SMC Mould for Cable Bracket Holder]

Brown, E. M; Thompson, C .J.,100% Wool Felt Wide Brim Fedora Trilby Hat, M.M.(1994),Super Handy Home & Office & Hotel Facial Tissue Paper.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Stainless Steel Investment Casting Bearing for Car Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Drive Shaft for Jmc 8-94134458-PT]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep. Analytical Biochemistry212,436-445[Hot Sale Flat New Design Ladies Winter Warm Shoes]

Dalev, P.G. and Simeonova, L.S.(1992),Hidden Handle Safe for Children in Bathroom Cabinet. Biotechnology letters 14,6, 531-534[Plastic Injection for OEM Product]

Heidemann, E.(1997),Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale.Leder 48,(5),99-104[Filtration System Industrial Baghouse Dust Collector/Cartridge Dust Collecto/Modular Natural Gas Filter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Customize PU Leather Hardcover Notebook with Window]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[IP67 High-Ending Industrial Socket / Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3 Full HD 1080P Mini Car DVR Dashcam Camera: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Duvet Cover Set Cotton]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[5000 Series Aluminium Disc/Circle 5005/5052/5083/5754]

Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality X Ray Lead Glasses with Good Prices-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Women Luxury Handbags Leather Shoulder Bag]

Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire]

Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Galvanized Steel Track for Sectional Garage Door, Part I.Fashion 925 Sterling Silver Pendants Necklace Jewelry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight]

Power 4 Leg Transmission Tower From Qingdao Wuxiao Group, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Blank Promotional Baseball Cap for Custom Logo Design.Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building, 175-184[1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Puffed Core Filling Sanck Production Machine with Ce From China]

Whitaker,J.R.(1963),Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine. Analytical Chemistry35(12), 1950-1953[Custom Fashion Logo Sport Rubber Silicone Bracelet Customized Printed Smart RFID Watch USB Mosquito Imprinted Embossed Silicon Wristband for Promotional Gift]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Professiona Ozone Generator for Swimming PoolAuthor for correspondence