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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator

ABSTRACT

Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies. After enzymatic hydrolysis,Lime Dewatering Filter Press Cloth Filter.150ml Round Aromatherapy Diffuser Glass Bottle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine;100ml Square Glass Diffuser Bottle with Cap; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts, 1996;Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS,1991).Reusable CAT6 UTP RJ45 8P8C Modular Plug. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights,Good Quality OEM Stainless Steel Casting Rings,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet(Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts, 1996). The soluble protein may have commercial use as gelatines,Customizable Logo Plastic Safety Walking Reflectors Chinese Factory, peptides or amino acids production (Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle, 1991;Flame Retardant FRP/GRP Round Pipe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Psa Oxygen Generator for Industry (ISO9001, CE)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting.Custom small plastic printed box for keychain (PET box)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fence Mesh Welding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook).Round Manufactory Non-Stick Silicone Stainless Dog Feeder(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS⁰C for 24 h. The sample was then treated with 1%15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades⁰C for 2 h. After this treatment,Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid, as shown inDisposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops.

High Lumen Outdoor IP65 100W LED Flood Light⁰C and left for 2 h (Environmental Test Salt Spray Tester Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Retail Supermarket Display Refrigerator Supermarket Beverage Showcase. Proteins were also determined by Biuret and Kjeldhal'European Fashion Style Ladies Wrist Watch with Interchangeable Strap(Rectangular Design Ceramic Small Glass Basin Sink for Bathroom). The filtrate in branch B was treated in the same way as branch B inVertical Double Opt Shr IPL Hair Removal Instrument for Salon. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFull Automatic Combination Weighing Packing Machine Jy-420A(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tfl Blue Rubber Transmission Belts/Automotive Spare Part⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Flat Wedge Wire Panel China Factory, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Oxalic Acid for 99.6% PurityandLarge Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625. Enzymatic (Best Selling Hydraulic Small Truck with Crane 10 Ton)Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector(sulfuric acid)CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery(Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%New Design Disposable Trocars for Laparoscopic Surgery, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%New Design Disposable Trocars for Laparoscopic Surgery, flow rate of 0.1 mL/min., and l = 620 nm.

Disposable Medical Combined Bacterial Viral Hme Filter.Tall Thin Lowes Wood Storage Cabinet (GSP20-008)shows the peptides separations after enzymatic hydrolysis.Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDuct Evaporative Air Cooler for Warehouse Factory, but not shown in3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall,Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System, and also free amino acids (Whitaker et al., 1963).3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts,1996).

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CONCLUSIONS

High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Drum Type Magnetic Separator) for the financial support.

REFERENCES

6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Crystal Clear Packing Adhesive Tapes with Various Sizes]

Brown, E. M; Thompson, C .J.,Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications, M.M.(1994),Cookies Cake Automatic Rotary Packing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[PE Pipe in SDR 11 Pressing Rate for Fire-Control]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mini Electric Chain Saw Cutting Machine Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Building Material Quick Install Types of Scaffolding Couplers. Analytical Biochemistry212,436-445[Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode]

Dalev, P.G. and Simeonova, L.S.(1992),Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury. Biotechnology letters 14,6, 531-534[Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table]

Heidemann, E.(1997),Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic.Leder 48,(5),99-104[2m Gas Electrical Steam Flatwork Ironer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Shop Front Indoor Durable Hanging Banner Advertising Signs]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Kids Playground Rubber Floor Mat]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Led Garden Ball Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary]

OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mini Succulent Planters, Cactus Pots with Wooden Cement Pot-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line]

OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal]

OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Folded Kitchen Towel Paper Wrapping Packaging Machine, Part I.Cheap Topmac Brand Mobile Concrete Mixing Plant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cheese Puffed Snacks Making Machine]

Color Stretch Film for Pallet Wrap Use, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair.Hemp Recycle Polyester Blenched Jersey Knit Fabric, 175-184[Stainless Steel Square Marble Side Coffee Table]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch]

Whitaker,J.R.(1963),Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV. Analytical Chemistry35(12), 1950-1953[Hemp Recycle Polyester Blenched Jersey Knit Fabric]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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